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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #346731

Research Project: Genetic and Environmental Factors Controlling Aflatoxin Biosynthesis

Location: Food and Feed Safety Research

Title: Effect of water activity, temperature, and carbon dioxide on the Aspergillus flavus transcriptome and aflatoxin B1 production

Author
item Gilbert, Matthew
item Medina-vaya, Angel - Cranfield University
item Mack, Brian
item Lebar, Matthew
item Rodriguez, Alicia - Cranfield University
item Bhatnagar, Deepak
item Magan, Naresh - Cranfield University
item Obrian, Gregory - North Carolina State University
item Payne, Gary - North Carolina State University

Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Other
Publication Acceptance Date: 3/27/2017
Publication Date: 3/27/2017
Citation: Gilbert, M.K., Medina-Vaya, A., Mack, B.M., Lebar, M.D., Rodriguez, A., Bhatnagar, D., Magan, N., Obrian, G., Payne, G. 2017. Effect of water activity, temperature, and carbon dioxide on the Aspergillus flavus transcriptome and aflatoxin B1 production. National Center for Biotechnology Information (NCBI). Accession: PRJNA380582.

Interpretive Summary:

Technical Abstract: Aspergillus flavus is a opportunistic fungus that has the potential to colonize several crops, including maize, peanuts and cotton. A. flavus colonization may result in the secretion of mycotoxins, of which the most prominent is aflatoxin. Temperature, water availability and carbon dioxide levels are three environmental factors that can influence fungal development. In this study we used RNA sequencing to better understand the transcriptomic response of the fungus to aw, temperature, and elevated carbon dioxide levels. The raw sequence reads provided here are from in situ experiments where maize kernels were infected with A. flavus spores and grown for several days in different environmental conditions. The conditions were: temperature: 30°C and 37°C, water activity: 0.91 aw and 0.99 aw, and carbon dioxide levels: 350 ppm, 650 ppm, and 1000 ppm. The conditions were tested in every possible combination of conditions comprising 27 different SRA Experiments.