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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Bio-oils Research » Research » Publications at this Location » Publication #342783

Research Project: Replacement of Petroleum Products Utilizing Off-Season Rotational Crops

Location: Bio-oils Research

Title: Acetyl diacylglycerol produced by modified camelina (Camelina sativa)

item Evangelista, Roque
item OHLROGGE, JOHN - Michigan State University
item Isbell, Terry
item Moser, Bryan
item DURRETT, TIMOTHY - Kansas State University
item Cermak, Steven - Steve

Submitted to: Association for the Advancement of Industrial Crops Conference
Publication Type: Abstract Only
Publication Acceptance Date: 6/23/2017
Publication Date: 9/10/2017
Citation: Evangelista, R.L., Ohlrogge, J.B., Isbell, T.A., Moser, B.R., Durrett, T., Cermak, S.C. 2017. Acetyl diacylglycerol produced by modified camelina (Camelina sativa) [abstract]. Association for the Advancement of Industrial Crops International Conference. p. 52.

Interpretive Summary:

Technical Abstract: Acetyl diacylglyceride (Acetyl-TAG) is a component of a commercial product, ACETEM, manufactured by transesterification reaction of triglycerides, glycerol, and triacetin or by acetylation of mono- and diglycerides with acetic acid anhydride. ACETEM is commonly used as foaming agents and coatings in food, lubricants in food processing, emulsifiers in cosmetic preparations, slip agents in paper products, and as plasticizer in plastics processing. Acetyl-TAG can now be produced by camelina (Camelina sativa), which was engineered by incorporating the diacylglycerol acetyltransferase gene isolated from Burning Bush (Euonymus alatus). The oil was extracted from the seeds by prepressing followed by hexane extraction. The oil was degummed, neutralized, and bleached. The acetyl-TAG was separated from the refined oil by distillation. A centrifugal-type laboratory still running at 190 deg C and 0.07 mbar vacuum pressure produced high-purity acetyl-TAG distillate but yield was <10%. The laboratory wiped-film short-path still operated at 250 deg C and 0.0005 mbar recovered most of the acetyl-TAG in the oil. The distilled acetyl-TAG is yellowish in color and contained some diacylglycerides and trace amounts of free fatty acids and other unidentified impurities. The distilled acetyl-TAG had higher oxidative stability index (OSI, 3.5 h induction period) than the conventional camelina oil (2.8 h IP). The distilled acetyl-TAG had slightly lower kinematic viscosity (24.4 vs. 25.7 mm/s at 40 deg C) and cloud point (-3 vs. 3 deg C) than the conventional oil. Their pour points were the same (-9 deg C).