Location: Healthy Processed Foods ResearchTitle: Effects of cyclodextrin glycosiltransferase modified starch and cyclodextrins on plasma glucose and lipids metabolism in mice
|DURA, ANGELA - Instituto De Agroquimica Y Technologia De Alimentos|
|Yokoyama, Wallace - Wally|
|ROSELL, CRISTINA - Instituto De Agroquimica Y Technologia De Alimentos|
Submitted to: Journal of Drug Design and Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/15/2017
Publication Date: 5/16/2017
Citation: Dura, A., Yokoyama, W.H., Rosell, C. 2017. Effects of cyclodextrin glycosiltransferase modified starch and cyclodextrins on plasma glucose and lipids metabolism in mice. Journal of Drug Design and Research. 4(5):1051.
Interpretive Summary: Starch is a polymer of glucose and glucose is released by enzymatic digestion in the intestine and absorbed into the blood stream. Enzyme treatments of starch usually involve heat treatment to gelatinize the starch. Gelatinization results in disruption of intramolecular bonds and absorption of water to facilitate enzyme access and action. In this report, ungelatinized starch was modified by an enzyme to produce short oligomers. The oligomers were cyclized by the same enzyme. In an animal model, the resulting hydrolyzed starch and cyclodextrins extended the time required to absorb glucose suggesting a slower rate of digestion. Blood cholesterol was also reduced.
Technical Abstract: The potential functional and nutritional benefits of granular starch treated with cyclodextrin glycosyltransferase (CGTase) and the released cyclodextrins (CDs) were explored in in vivo studies. The metabolic effects of diets in the C57BL/6J mouse containing native and enzymatically modified corn starch by CGTase with or without hydrolysis products were studied. The hydrolysis products were oligosaccharides and CDs, mainly ß-CD. Blood glucose concentration at 2 hours was higher suggesting that enzymatically treated starches containing CDs slowed digestion resulting in a longer period of absorption and consequently higher blood glucose levels at the later times. The modified starch with CDs tended to increase HDL-cholesterol levels while decreasing VLDL-cholesterol levels. The CGTase modified starches lowered total and cholesterol ester values in liver and decreased fecal fat extraction. The inclusion of CGTase modified granular starches in the presence of their hydrolysis products may be useful to prevent obesity and other related metabolic diseases, offering an alternative healthy ingredient to the food industry.