|ORENDAY-ORTIZ, JOSE - Washington State University|
Submitted to: AACC International
Publication Type: Abstract Only
Publication Acceptance Date: 6/6/2017
Publication Date: 10/20/2017
Citation: Orenday-Ortiz, J.M., Kiszonas, A., Morris, C.F. 2017. Mapping quantitative trait loci for a unique 'super soft' kernel trait in soft white wheat. AACC International. Available: https://aaccnet.confex.com/aaccnet/2017/meetingapp.cgi/Home/0.
Interpretive Summary: N/A
Technical Abstract: Wheat (Triticum sp.) kernel texture is an important factor affecting milling, flour functionality, and end-use quality. Kernel texture is normally characterized as either hard or soft, the two major classes of texture. However, further variation is typically encountered in each class. Soft wheat varieties are utilized to make a wide diversity of baked products and Asian-style noodles. Therefore, variation in grain texture is an important quality parameter that can be exploited with novel kernel textures to provide new opportunities for food processing, and to modify the end-use quality of wheat. The present study examined a unique soft kernel trait in soft white wheat (T. aestivum L.), designated as ‘super soft’, segregating in a bi-parental mapping population. A set of 136 F3-derived F4 recombinant inbred lines (RILs) were grown at the Plant Growth Facilities (Pullman, WA), leaf tissue was collected, and DNA was extracted. DNA for RILs and parent plants was subjected to a high-density iSelect 90K single nucleotide polymorphism (SNP) array (Illumina) to construct a genetic linkage map for quantitative trait loci (QTL) associated with the super soft trait. F3 and F4 kernel hardness, weight, and diameter were determined by single kernel characterization system (SKCS). Super soft kernels exhibited SKCS hardness index (HI) values that were unusually soft, HI ˜ 10 or less; whereas normal soft kernels exhibited HI values ˜ 25. The observed distribution of HI values for kernels shows that most RILs were found to have an intermediate phenotype of both parents suggesting that they may carry alleles that when properly segregated can express the super soft trait. The identification of QTL for the super soft kernel texture trait provides wheat breeders with insight as to how the trait is inherited, and establishes a foundation for future breeding efforts.