Location: Location not imported yet.Title: Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids
|WATERS, RYAN - The Pirbright Institute
|Rieder, Aida - Elizabeth
|PEGA, JUAN - Instituto Nacional De Tecnologia Agropecuaria
|PEREZ-FILGUERA, MARIANO - Instituto Nacional De Tecnologia Agropecuaria
Submitted to: Journal of Immunological Methods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/5/2017
Publication Date: 11/1/2017
Citation: Kenney, M.A., Waters, R.A., Rieder, A.E., Pega, J., Perez-Filguera, M., Golde, W.T. 2017. Enhanced sensitivity in detection of antiviral antibody responses using biotinylation of foot-and-mouth disease virus (FMDV) capsids. Journal of Immunological Methods. 450:1-9. https://doi.org/10.1016/j.jim.2017.07.001.
Interpretive Summary: Foot-and-mouth disease virus (FMDV) is a small RNA virus that causes devastating disease in cattle and other cloven-hoofed animals worldwide. This study describes a novel method to examine the immune response to FMDV infection or vaccination. It also describes an novel procedure to label the virus and to detect anti-virus antibodies.
Technical Abstract: Analysis of the immune response to infection of livestock by foot-and-mouth disease virus (FMDV) is most often reported as the serum antibody response to the virus. While measurement of neutralizing antibody has been sensitive and specific, measurements of the quality of the antibody response are less robust. Determining the immunoglobulin (Ig) isotype of the serum antibody response provides a deeper understanding of the biology of the response and more sensitive methods for these assays will facilitate analyses of B cell mediated immunity. We tested the hypothesis that using the virus as the molecular probe could be achieved by adding tags to the surface of the FMDV capsid, and that would enhance sensitivity in assays for anti-FMDV antibody responses. The use of a FLAG-tagged virus in these assays failed to yield improvement whereas chemically biotinylating the virus capsid resulted in significant enhancement of the signal. Here we describe methods using biotinylated virus for measuring anti-viral antibody in serum and antibody secreting cells (ASCs) in blood that are sensitive and specific. Finally, we describe using the biotinylated virus in flow cytometry where such assays should greatly enhance the analysis of anti-virus antibody producing B cells, allowing the investigator to focus on only the FMDV specific B cells when analyzing the development of the B cell response to either infection or vaccination.