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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #337790

Research Project: Development of Genomic Tools to Study Ruminant Resistance to Gastrointestinal Nematodes (Bridge Project)

Location: Animal Genomics and Improvement Laboratory

Title: Chondriotin sulfate disaccharides as a bioactive compound modified the murine gut microbiome under healthy and stressed conditions

Author
item Liu, Fang - Ocean University Of China
item Zhang, Na - Ocean University Of China
item Li, Zhaojie - Ocean University Of China
item Wang, Xiong - Ocean University Of China
item Shi, Hongjie - Ocean University Of China
item Xue, Changhu - Ocean University Of China
item Li, Robert
item Tang, Qingjuan - Ocean University Of China

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/5/2017
Publication Date: 7/28/2017
Citation: Liu, F., Zhang, N., Li, Z., Wang, X., Shi, H., Xue, C., Li, R.W., Tang, Q. 2017. Chondriotin sulfate disaccharides as a bioactive compound modified the murine gut microbiome under healthy and stressed conditions. Scientific Reports. 7:6783. https://doi.org/10.1038/s41598-017-05860-6.
DOI: https://doi.org/10.1038/s41598-017-05860-6

Interpretive Summary: Chondriotin sulfate (CS), a class of long unbranched polysaccharides, plays an important role in organ development and host-parasite interactions. As a result, glycosamine, one of the CS precursors, has been promoted as a dietary supplement. Headaches, muscle pain, and gastrointestinal disturbances are among the common manifestations of people under severe stress, which is associated with gut inflammation and alterations in the gut microbial community. In this study, we investigated the beneficial effect of CS in regulating gut microbes under healthy and stressed conditions. Our results show that CS can ameliorate stress-induced gut inflammation. Our findings should facilitate the development of CS as a bioactive ingredient to boost immunity and restore gut microbial imbalance in the gut during stress.

Technical Abstract: Chondriotin sulfate (CS) has been widely used for medical and nutraceutical purposes due to its roles in maintaining tissue structural integrity. We investigated if CS disaccharides may act as a bioactive compound and modulate gut microbial composition in mice. Our data show that CS disaccharides supplementation for 16 days significantly reduced blood LPS in the mice experiencing exhaustive exercise stress. CS disaccharides partially restored total fecal short-chain fatty acids from the level significantly repressed in mice under the stress. Our findings demonstrated that CS was likely butyrogenic and resulted in a significant increase in fecal butyrate concentration. CS disaccharides had a profound impact on gut microbial composition, affecting the abundance of 13.6% and 7.3% Operational Taxonomic Units in fecal microbial communities in healthy and stressed mice, respectively. CS disaccharides reduced the prevalence of inflammatory Proteobacteria. Together, our findings demonstrated that CS may ameliorate stress-induced intestine inflammation. Furthermore, CS significantly increased intestinal Bacteroides acidifaciens population, indirectly exerting its immunomodulatory effect on the intestine. CS disaccharides had a significant impact on a broad range of biological pathways under stressed condition, such as ABC transporters, two-component systems, and carbohydrate metabolism. Our results will facilitate the development of CS as a bioactive neutraceutical.