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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #337516

Research Project: BIOCONTROL AGENT PRODUCTION AND DEPLOYMENT TECHNOLOGIES FOR THE INTEGRATED MANAGEMENT OF PLANT PATHOGENS

Location: Crop Bioprotection Research

Title: Bacillus swezeyi sp. nov. and Bacillus haynesii sp. nov., isolated from desert soil

Author
item Dunlap, Christopher
item Schisler, David - Dave
item Perry, Elizabeth - Wesleyan University
item Connor, Nora - Wesleyan University
item Cohan, Frederick - Wesleyan University
item Rooney, Alejandro - Alex

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/5/2017
Publication Date: 8/15/2017
Citation: Dunlap, C.A., Schisler, D.A., Perry, E.B., Connor, N., Cohan, F.M., Rooney, A.P. 2017. Bacillus swezeyi sp. nov. and Bacillus haynesii sp. nov., isolated from desert soil. International Journal of Systematic and Evolutionary Microbiology. 67:2720-2725. doi: 10.1099/ijsem.0.002007.
DOI: https://doi.org/10.1099/ijsem.0.002007

Interpretive Summary: This study describes a new bacterial species that was isolated from soil in Evolution Canyon III, Israel. Novel bacteria may possess unique and unusual biochemical properties that make them attractive candidates to explore their potential for new uses in agriculture or biotechnology. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for agricultural applications can be assessed in future studies.

Technical Abstract: Two isolates of Gram-positive, facultatively anaerobic, motile, rod-shaped, endospore-forming bacteria were identified during a survey of the diversity of Bacillus strains deposited in the Agriculture Research Service Culture Collection. These strains were originally isolated from soil in Evolution Canyon III (Israel) in a survey of ecological diversification in bacterial evolution. Phylogenetic analysis of the 16S rRNA gene of strains NRRL B-41294 and NRRL B-41327 determined they were closely related to members of the Bacillus licheniformis clade. The genome of each strain was sequenced, and further analysis indicated that the strains were unique species based on in silico DNA-DNA hybridization analyses. A phylogenomic analysis of the core genomes all members of the Bacillus subtilis group as well as NRRL B-41294 and NRRL B-41327 revealed that the latter formed a clade most closely related to the group that includes B. licheniformis and Bacillus sonorensis. In phenotypic characterization, both NRRL B-41294 and NRRL B-41327 were found to grow between 15 and 60 'C and tolerated up to 12% NaCl (w/v). The predominant cellular fatty acids were anteiso-C15':'0 and iso-C15':'0, and peptidoglycan from cell walls contained meso-diaminopimelic acid. The DNA G+C content was 45.7 mol% and 44.3 mol% for NRRL B-41327 and NRRL B-41294, respectively. Furthermore, each strain had a unique carbon utilization pattern that distinguished it from its nearest neighbors. Based upon the consensus of phylogenetic and phenotypic analyses, we conclude these strains represent two novel species within the genus Bacillus, for which the name Bacillus swezeyi sp. nov. is proposed, with type strain NRRL B-41294**T (=CCUG 6####**T) and the name Bacillus haynesii sp. nov. is proposed, with type strain NRRL B-41327**T (=CCUG 6####**T).