Location: Healthy Processed Foods ResearchTitle: Adaption of Ehrlich’s Reagent to a HPLC post-column reaction system for the quantification of limonoid glucosides (abstract)
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/22/2016
Publication Date: 4/2/2017
Citation: Breksa Iii, A.P., King, D.E., Vilches, A.M. 2017. Adaption of Ehrlich’s Reagent to a HPLC post-column reaction system for the quantification of limonoid glucosides [abstract].
Technical Abstract: Citrus limonoid glucosides are found in large quantities in citrus fruits and seeds. Characterization and quantification of these compounds is important because they contribute to citrus quality and are reported to be biologically active. Unlike other bioactive compounds (e.g., flavonoids) present in citrus, limonoid glucosides lack a specific chromophore and only poorly absorb light around 220 nm. HPLC-UV analytical methods used to quantitate limonoid glucosides rely on extensive sample clean up procedures or long chromatographic runs. To overcome these requirements, we developed a post-column reaction system in which Ehrlich’s Reagent is reacted selectively with limonoid glucosides to form red to orange colored products that are measured at 523 nm. Method development experiments were conducted using limonin glucoside, the most abundant limonoid glucoside and a material that is commercially available. Limit of detection for the method was determined to be 50 ng of limonin glucoside. Limonin glucoside was found to be a suitable surrogate for quantitation of other limonoid glucosides. This method was used to characterize the limonoid glucoside content of juices from a variety of citrus cultivars.