|BOEHM, JEFFREY - Washington State University|
Submitted to: Wheat Genetics International Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/30/2017
Publication Date: 5/24/2017
Citation: Morris, C.F., Kiszonas, A., Boehm, J.D. 2017. Genetic mapping of flavor loci in wheat. Wheat Genetics International Symposium Proceedings. BOKU - University of Natural Resources and life Sciences, Vienna, Austria. 104. URL: http://iwgs2017.boku.ac.at/.
Technical Abstract: Flavor is an essential aspect of consumer acceptance, especially with whole-wheat foods. However, little if any selection is performed during breeding of new wheat cultivars for flavor, and little is known regarding the genetics of flavor. Our research is aimed at identifying genes that impart either a desirable (Yummy) or undesirable (yucky) flavor to wheat grain. We have developed a mouse model system to test the consumption preference of grain (1). Grain of two varieties is mixed and provided to 10 replicate mice over two sequential 24-hr periods. Consumption data are analyzed using Student’s t statistic. Initial studies identified kernel hardness and bran color as significantly influencing consumption preference. However, dramatic differences were observed within texture and color classes. Initial experiments tested ‘single elimination’ and ‘round robin’ tournament designs; both were effective but not well adapted to genetic mapping. More recently, a system of comparing ‘experimental’ lines to a common check was developed wherein the lines could be derived from doubled haploids (DHs), recombinant inbred lines (RILs) or collections of varieties. A major hurdle was overcome by utilizing the actual t statistic as a consumption phenotype (2). Most recently, this model system has been used in two mapping studies: Clarks Cream x NY6432-18 (‘CCxNY’, hard by soft white winter, 78 RILs) (3) and Louise x Yumai34 (‘LxY’, soft white springs, 373 DHs). CCxNY employed an existing marker dataset with 102 RFLPs; LxY used a genotyping-by-sequencing markers. For CCxNY, using Pinb and hardness as covariates, QTL were identified on 3B, 3D, 5A, 6B, and group 7. For LxY, dominant markers from Louise (Yummy parent) were on 4DL and 6DL; SNP markers were on 5AS, 4BS, and 2D/4BL.