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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #335485

Research Project: ENHANCING GENETIC MERIT OF RUMINANTS THROUGH GENOME SELECTION AND ANALYSIS

Location: Animal Genomics and Improvement Laboratory

Title: Construction of PRDM9 allele-specific recombination maps in cattle using large-scale pedigree analysis and genome-wide single sperm genomics

Author
item ZHOU, YANG - Northwest Agricultural & Forestry University
item SHEN, BOTONG - University Of Maryland
item JIANG, JICAI - University Of Maryland
item PADHI, ABINASH - University Of Maryland
item PARK, KI-EUN - University Of Maryland
item OSWALT, ADAM - Select Sires, Inc
item SATTLER, CHARLES - Select Sires, Inc
item TELUGU, BHANU - University Of Maryland
item CHEN, HONG - Northwest Agricultural & Forestry University
item Cole, John
item Liu, Ge - George
item MA, LI - University Of Maryland

Submitted to: DNA Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/27/2017
Publication Date: 11/27/2017
Citation: Zhou, Y., Shen, B., Jiang, J., Padhi, A., Park, K., Oswalt, A., Sattler, C., Telugu, B.P., Chen, H., Cole, J.B., Liu, G., Ma, L. 2017. Construction of PRDM9 allele-specific recombination maps in cattle using large-scale pedigree analysis and genome-wide single sperm genomics. DNA Research. 25(2):183–194. https://doi.org/10.1093/dnares/dsx048.
DOI: https://doi.org/10.1093/dnares/dsx048

Interpretive Summary: Recombination during meiosis is facilitated by chromosomal crossover. Using large-scale analysis of pedigree and sperm-typing data, we systematically stuided PRDM9's impacts on recombination in individual cattle. These results fill our knowledge gaps and provide the foundation for future studies of recombination's functional role in genome evolution and selection. Farmers, scientist, and policy planners who need improve animal health and production based on genome-enable animal selection will benefit from this study.

Technical Abstract: Meiotic recombination is a major driving force in promoting genetic and phenotypic variations in sexually reproducing organisms. Although PRDM9 is known to modulate the binding-specificity and location of recombination hotspots in humans and mice, its role, especially in domesticated animals like cattle, is largely uncharacterized. Using large-scale pedigree data and sperm-typing techniques, here we report PRDM9 allele-specific recombination maps and hotspots across the cattle genome, dominant effects of PRDM9 alleles on recombination patterns, and strong associations between these hotspots and unique mutations at functional sites in the PRDM9 zinc fingers. Comparing recombination maps built from sperms and pedigree data of a single bull, we identified two regions with slightly different recombination rate, although the genome-wide recombination rate is the same between sperm and pedigree. Our results provided an example for the construction of recombination maps for domesticated animals and enhanced our understanding of the impacts of PRDM9 on meiotic recombination.