|ZHANG, ZHOU - Collaborator|
|LI, MINGXUN - Collaborator|
|HU, XINDE - Chinese Academy Of Sciences|
|CAI, HANGFANG - Collaborator|
|LIU, MEI - Collaborator|
|SUN, YUJIA - Collaborator|
|HUANG, YONGZHEN - Collaborator|
|DANG, RUIHUA - Collaborator|
|LAN, XIABYONG - Collaborator|
|LEI, CHUZHAO - Collaborator|
|Liu, Ge - George|
|Li, Congjun - Cj|
|HONG, CHEN - Collaborator|
Submitted to: Oncotarget
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/5/2017
Publication Date: 1/2/2018
Citation: Zhang, Z., Li, M., Hu, X., Cai, H., Liu, M., Sun, Y., Huang, Y., Dang, R., Lan, X., Lei, C., Liu, G., Li, C., Hong, C. 2018. Characterization of candidate genes for bovine adipogenesis reveals differences of TUSC5 isoforms caused by novel alternative splicing. Oncotarget. 9(1): s287-s298. https://doi.org/10.18632/oncotarget.23482.
Interpretive Summary: Numerous genes related to lipid deposition have been studied intensively. However, different transcripts generated by alternative splicing have led to new insights to reconsider gene functions. We investigated the alternative splicing events related to TUDC5, a gene with lipid deposition activities. We found that the two transcripts and isoforms of TUDC5 gene were expressed differently, have different cellular localization and may have different functions. This study helped us to understand the functional differences caused by alternative splicing.
Technical Abstract: Numerous genes related to lipid deposition have been studied intensively. However, different transcripts generated by alternative splicing have led to new insights to reconsider gene functions. In this study, we observed not all the genes induced by peroxisome proliferator activated receptor gamma (PPARG) agonist (rosiglitazone) could be induced by PPARG2 in bovine adipocytes by screening 12 genes robustly expressed in bovine adipose tissue. Alternative splicing events of two genes regulated by PPARG2 — cell death-inducing DFFA-like effector c (CIDEC) and tumor suppressor candidate 5 (TUSC5) — were further investigated. Consistent results for two transcripts/isoforms of CIDEC in the expression profile and subcellular localization analyses were observed. However, obvious differences were seen for the two transcripts/isoforms of TUSC5. TUSC5 isoforms were expressed differently in various tissues and during the adipogenesis process. The expression of the novel TUSC5 transcript was significantly delayed by the 87 nt insertion. The subcellular localization analyses showed that TUSC5 isoforms existed in the endplasmic reticulum but with different localization and no interaction with CIDEC isoforms. In summary, this study provided candidate genes for bovine adipogenesis analysis and promoted the understanding of the functional differences caused by alternative splicing.