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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #333383
Research Project: Use of Microorganisms to Manage Weeds and Insect Pests in Turf and Agricultural Systems

Location: Crop Bioprotection Research

Title: Chengkuizengella sediminis gen. nov. sp. nov., isolated from sediment

Author
item CAO, WEN-RUI - Chinese Academy Of Sciences
item GUO, LING-YUN - Shandong University
item DU, ZONG-JUN - Shandong University
item DAS, ANINDITA - Chinese Academy Of Sciences
item SAREN, GAOWA - Chinese Academy Of Sciences
item JIANG, MING-YU - Chinese Academy Of Sciences
item Dunlap, Christopher
item Rooney, Alejandro - Alex
item YU, XIN-KE - Chinese Academy Of Sciences
item LI, TIE-GANG - First Institute Of Oceanography

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/4/2017
Publication Date: 8/8/2017
Publication URL: http://handle.nal.usda.gov/10113/5934962
Citation: Cao, W., Guo, L., Du, Z., Das, A., Saren, G., Jiang, M., Dunlap, C.A., Rooney, A.P., Yu, X., Li, T. 2017. Chengkuizengella sediminis gen. nov. sp. nov., isolated from sediment. International Journal of Systematic and Evolutionary Microbiology. 67:2672–2678. doi: 10.1099/ijsem.0.002006.

Interpretive Summary: ARS researchers from Peoria, IL collaborated with scientists from the Chinese Academy of Sciences and Shandong University in Weihai, China to characterize a novel bacterium found in marine environment. This manuscript reports on the characterization of a new genus and species of bacteria, Elvibacillus sedimins, that grows in high salt concentrations. This species possesses novel and unusual biochemical characteristics, which makes it attractive for various agricultural and biotechnological purposes. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for biological control applications can be assessed in future studies.

Technical Abstract: A Gram-strain-positive, aerobic, motile, endospore-forming bacterium, designated strain J15A17T, was isolated from sediment of the South China Sea. The strain was oxidase-positive and catalase-negative. Optimal growth occurred at 33', pH 7.5 and in the presence of 3% (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, the strain showed closest similarity (92.81 %) with Paenibacillus puldeungensis strain CAU 9324T. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate forms a separate branch within the family Paenibacillaceae, with Cohnella as the closest related genus. The DNA G+C content was 37.4 mol%. The strain contained MK-7 as the sole respiratory quinone; anteiso-C15: 0 and iso-C16: 0 as the major cellular fatty acids; and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid, and four unidentified phospholipids. Phylogenetic, physiological, biochemical, and morphological differences between strain J15A17T and its closest relatives in the generas Cohnella, Fontibacillus, and Paenibacillus suggest that strain J15A17T (=KCTC 33759T=MCCC 1H00137T) represents as the type strain of a novel species in a new genus within the family Paenibacillaceae, Elvibacillus sedimins gen. nov. sp. nov.