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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #332363

Research Project: Use of Microorganisms to Manage Weeds and Insect Pests in Turf and Agricultural Systems

Location: Crop Bioprotection Research

Title: Salibacter halophilus gen. nov., sp. nov., isolated from a saltern

Author
item Lu, De-chen - Shandong University
item Xia, Jun - Shandong University
item Dunlap, Christopher
item Rooney, Alejandro - Alex
item Du, Zong-jun - Shandong University

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/15/2017
Publication Date: 6/8/2017
Citation: Lu, D., Xia, J., Dunlap, C.A., Rooney, A.P., Du, Z. 2017. Salibacter halophilus gen. nov., sp. nov., isolated from a saltern. International Journal of Systematic and Evolutionary Microbiology. 67:1784–1788. doi: 10.1099/ijsem.0.001807.
DOI: https://doi.org/10.1099/ijsem.0.001807

Interpretive Summary: ARS researchers from Peoria, IL collaborated with scientists from Shandong University in Weihai, China to characterize a novel bacterium found in marine environment. This manuscript reports on the characterization of a new species of bacteria Salimarina halophila, that grows in high salt concentrations. This species possesses novel and unusual biochemical characteristics, which makes it attractive for various agricultural and biotechnological purposes. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for biological control applications can be assessed in future studies.

Technical Abstract: A Gram-stain-negative and facultatively anaerobic bacterium, JZ3C34**T, was isolated from a saltern near Feicheng, China (36°8'24.45" E, 116°49'22.46" N). Cells of strain JZ3C34**T were 0.3-0.4µm wide and 1.5-2.0µm long, catalase-positive and oxidase-negative. Colonies were transparent, orange and approximately 1.0 mm in diameter. Growth occurred at 20-50 °C (optimally at 33-37 °C), pH 6.5-8.5 (optimally at 7.0-8.0) and in the presence of 2-18 % (w/v) NaCl (optimally in 6-8 % (w/v) NaCl). Phylogenetic analysis of the 16S rRNA gene indicated that strain JZ3C34**T was a member of the family Cryomorphaceae within the order Flavobacteriales and the most closely related species was Owenweeksia hongkongensis DSM 17368**T, although the relationship was fairly distant and consistent with the taxa being members of different genera (89.2 % 16S rRNA gene sequence similarity). The major respiratory quinone of strain JZ3C34**T was found to be MK-7, and the dominant fatty acids were found to be iso-C15:0 and iso-C15:1 G. The major polar lipids were two kind of unidentified lipids (L2 and L3) and phosphatidylethanolamine (PE), and the genomic DNA G+C content was 39.6 mol %. The polyphasic taxonomy traits presented here clearly place the new strain as a novel species within a new genus of the family Cryomorphaceae, for which we propose the name Salimarina halophila gen. nov., sp. nov. The type strain is JZ3C34**T (=KCTC 52047**T =MCCC 1K02288**T).