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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #331404

Research Project: Use of Microorganisms to Manage Weeds and Insect Pests in Turf and Agricultural Systems

Location: Crop Bioprotection Research

Title: Psychroflexus saliphilus sp. nov., isolated from a marine solar saltern

Author
item Jin, Sang - Shandong University
item Jun, Xia - Shandong University
item Dunlap, Christopher
item Rooney, Alejandro - Alex
item Zong-jun, Du - Shandong University

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/6/2016
Publication Date: 12/1/2016
Citation: Jin, S., Jun, X., Dunlap, C.A., Rooney, A.P., Zong-Jun, D. 2016. Psychroflexus saliphilus sp. nov., isolated from a marine solar saltern. International Journal of Systematic and Evolutionary Microbiology. 66:5124-5128. doi: 10.1099/ijsem.0.001482.

Interpretive Summary: ARS researchers from Peoria, IL collaborated with scientists from Shandong University in Weihai, China to characterize a novel bacterium found in marine environment. This manuscript reports on the characterization of a new species of bacteria Psychroflexus saliphilus, that grows in high salt concentrations. This species possesses novel and unusual biochemical characteristics, which makes it attractive for various agricultural and biotechnological purposes. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for biological control applications can be assessed in future studies.

Technical Abstract: A Gram-stain-negative, non-motile, rod-shaped, orange pigmented strain, WDS4A13**T, was isolated from a marine solar saltern in Weihai, China. WDS4A13**T grows optimally at pH at 7.0-8.0, 33°C, with 6% (w/v) NaCl. The polar lipid profile of the novel isolate consisted of two unidentified phospholipids (PL 1, PL2), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), a glycolipid (GL 1), and an unidentified lipid (L3) as the major polar lipids. The predominant menaquinone is MK-6 and iso-C15:1 G, (25.9%), iso-C15:0 (22.0%), C15:0 (11.8%) are the major fatty acids. The DNA G+C content is 35.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain WDS4A13**T belonged to the genus Psythroflexus. The levels of similarity between the 16S rRNA gene sequence of strain WDS4A13**T and those of the type strains of recognized Psychroflexus species are 92.0-97.3%. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain WDS4A13**T represents a novel species within the genus Psychroflexus, for which the name Psychroflexus saliphilus sp. nov. is proposed. The type strain is WDS4A13**T (=MCCC 1H00134**T =KCTC 52043**T).