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Research Project: Soft Kernel Durum Wheat: Removing the Culinary Constraints of this Genetically Rich Cereal Species

Location: Wheat Health, Genetics, and Quality Research

Title: Characterization of the low-molecular-weight glutenin subunit gene family members using a PCR-based marker approach

Author
item Ibba, Maria Itria - Washington State University
item Boehm, Jeffrey - Washington State University
item Kiszonas, Alecia
item Morris, Craig

Submitted to: American Association of Cereal Chemists Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 9/8/2016
Publication Date: 10/25/2016
Citation: Ibba, M., Boehm, J., Kiszonas, A., Morris, C.F. 2016. Characterization of the low-molecular-weight glutenin subunit gene family members using a PCR-based marker approach. American Association of Cereal Chemists Meetings. http://www.aaccnet.org/meetings/Documents/2016Abstracts/aacc2016abs151.htm

Interpretive Summary:

Technical Abstract: Low-molecular-weight glutenin subunits (LMW-GS) are a class of seed storage proteins that play a major role in the determination of the processing quality of wheat flour. The LMW-GS are encoded by multi-gene families located on the short arms of the homoeologous group 1 chromosomes, at the Glu-A3, Glu-B3 and Glu-D3 loci. Generally, more than 15 genes are present in a single bread wheat cultivar. Because of the genic complexity of these loci and the lack of efficient methods for the LMW-GS protein allele identification, the role of each LMW-GS allele in the end-use quality has not been clearly established. In the present study a PCR-based molecular marker approach has been used to identify the LMW-GS gene alleles present in different hexaploid wheat cultivars. Analysis of a set of standard wheat cultivars with known LMW-GS alleles revealed the potential of this marker system for the unambiguous identification of the LMW-GS protein alleles. Moreover, the application of this system to three different populations segregating for the LMW-GS gene alleles showed the presence of intra-locus recombination suggesting a possible revision of the concept of a “single locus” for all the three Glu-3 loci. The results of this study will help to define the LMW-GS profile of different hexaploid wheat cultivars and to clarify the contribution of each LMW-GS gene alleles in the control of the end-use quality.