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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #327652

Research Project: BIOCONTROL AGENT PRODUCTION AND DEPLOYMENT TECHNOLOGIES FOR THE INTEGRATED MANAGEMENT OF PLANT PATHOGENS

Location: Crop Bioprotection Research

Title: Longibacter salinarum gen. nov., sp. nov., isolated from a marine solar saltern

Author
item Xia, Jun - Shandong University
item Dunlap, Christopher
item Weiler, Lina
item Rooney, Alejandro - Alex
item Chen, Guan-jun - Shandong University
item Du, Zong-jun - Shandong University

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/23/2016
Publication Date: 9/1/2016
Citation: Xia, J., Dunlap, C.A., Weiler, L., Rooney, A.P., Chen, G., Du, Z. 2016. Longibacter salinarum gen. nov., sp. nov., isolated from a marine solar saltern. International Journal of Systematic and Evolutionary Microbiology. 66:3287-3292. doi:10.1099/ijsem.0.001190.

Interpretive Summary: This manuscript reports on the characterization of a new species of bacteria, Longibacter halophile, that grows in high salt concentrations. This species possesses novel and unusual biochemical characteristics, which makes it attractive for various agricultural and biotechnological purposes. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for biological control applications can be assessed in future studies.

Technical Abstract: A bacterial strain, designated WDS2C18**T, was isolated from a marine solar saltern in the coast of Weihai, Shandong Province, PR China. Cells of strain WDS2C18**T were long rod-shaped, red, and approximately 6.0–12.0 µm in length and 0.3–0.6 µm in width. The strain was Gram-stain-negative, facultatively anaerobic, heterotrophic, catalase-positive, and oxidase-negative. Growth occurred in 2–20% (w/v) NaCl, at 20–50°C and pH 6.5–8.5. Optimal growth was observed at 40°C, pH 7.5–8.0, with 8–12% (w/v) NaCl. Nitrate was not reduced. Glycerol, sucrose, starch, and D-mannitol stimulated growth, but not glucose, D-fructose, D-galactose, D-lactose, D-maltose, D-mannose, D-xylose, D-ribose, D-arabinose, L-rhamnose, or D-cellobiose. The G+C content of the genomic DNA was 60.6 mol% (HPLC). The sole methyl naphthoquinone was MK-7 and the predominant cellular fatty acids (>10%) were iso-C15:0 2-OH/C16:1 '7c; iso-C17:1 '9c; iso-C15:0; and iso-C17:0. The predominant polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), glycolipid (GL), 3 unidentified phospholipid (PL), and 2 unidentified lipid (L). Phylogenetic analysis, based on 16S rRNA gene sequences, demonstrated that strain WDS2C18**T was affiliated with the phylum Bacteroidetes. The closest related neighbors were species of the genera Salisaeta and Longimonas, strain WDS2C18**T shared highest 16S rRNA gene sequence similarities to Salisaeta longa DSM 21114**T (91.7%) and Longimonas halophila SYD6**T (90.8%) and less than 90.0% to other species of the family Rhodothermaceae. On the basis of these phenotypic and phylogenetic data, strain WDS2C18**T should be classified as a novel genus and species within the family Rhodothermaceae, for which the name Longibacter halophila gen. nov., sp. nov. is proposed. The type strain is WDS2C18**T (=MCCC 1H00132**T = KCTC 52045**T).