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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #326702

Research Project: Genetic Enhancement of Allium, Cucumis, and Daucus Germplasm

Location: Vegetable Crops Research

Title: A truncated F-box protein confers the dwarfism in cucumber

Author
item LIN, TAO - Chinese Academy Of Agricultural Sciences
item WANG, SHENHAO - Chinese Academy Of Agricultural Sciences
item ZHONG, YANG - Chinese Academy Of Agricultural Sciences
item GAO, DONGLI - Chinese Academy Of Agricultural Sciences
item CUI, QINGZHI - University Of Wisconsin
item CHEN, HUIMING - University Of Wisconsin
item ZHANG, ZHONGHUA - Chinese Academy Of Agricultural Sciences
item SHEN, HUOLIN - Chinese Agricultural University
item Weng, Yiqun
item HUANG, SANWEN - Chinese Academy Of Agricultural Sciences

Submitted to: Journal of Genetics and Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/13/2016
Publication Date: 1/24/2016
Publication URL: http://handle.nal.usda.gov/10113/62495
Citation: Lin, T., Wang, S., Zhong, Y., Gao, D., Cui, Q., Chen, H., Zhang, Z., Shen, H., Weng, Y., Huang, S. 2016. A truncated F-box protein confers the dwarfism in cucumber. Journal of Genetics and Genomics. 43(4):223-226. doi: 10.1016/j.jgg.2016.01.007.

Interpretive Summary: Dwarfism is an important plant architecture trait for cucumber breeding. In this study, we characterized an EMS-induced dwarf mutant 406M in cucumber, which exhibited a shorter internode length as compared with its wild type. In a BC1F2 population from the cross of 406M with its wild type line 406, the segregation ratio fit 3:1 indicating the dwarf mutation was controlled by a single recessive gene. To identify the genetic basis of this mutation, we constructed a normal (N) and a mutant (M) bulk consisting 20 normal and dwarf plants, respectively, which were re-sequenced. SNPs were identified between the two pools, and SNP-index graphs were calculated and plotted. '(SNP-index) distribution identified 4 SNPs in cucumber chromosome 4 that were associated with the dwarf phenotype. Linkage analysis with the 4 SNPs in the BC1F2 population delimited the dwarf locus to a 2.04 Mb interval between the two markers dCAPS-SNP4G19847214 and dCAPS-SNP4G21882884. In this region, only dCAPS-SNP4G21398058 is co-segregating with the dwarfism phenotype, in which the cucumber gene Csa4G641640 encoding an F-Box protein was annotated. The SNP (C-to-T) mutation resulted in premature termination of the protein. Thus, genetic and genomic analysis pinpointed that the truncated F-Box Protein Csa4P641640 that was responsible for the dwarf phenotype in 406M.

Technical Abstract: Dwarfism is an important plant architecture trait for cucumber breeding. In the present study, we identified a dwarf mutant 406M in cucumber which showed a shorter internode length as compared with its wild type. In a BC1F2 population from the cross of 406M with its wild type parental line 406, the segregation ratio fit 3:1 indicating the dwarf mutation was controlled by a single recessive gene. To identify the genetic basis of this mutation, we constructed a normal (N) and a mutant (M) bulk consisting 20 normal and dwarf plants, respectively. We re-sequenced the two DNA pools as well as the mutant line. SNPs between the two pools were identified, and SNP-index graphs were calculated and plotted. '(SNP-index) distribution identified 4 SNPs in cucumber chromosome 4 that were associated with the dwarf phenotype. Linkage analysis with the 4 SNPs in the BC1F2 population delimited the dwarf locus to a 2.04 Mb interval between the two markers dCAPS-SNP4G19847214 and dCAPS-SNP4G21882884. In this region, only dCAPS-SNP4G21398058 is co-segregating with the dwarfism phenotype, in which the cucumber gene Csa4G641640 encoding an F-Box protein was annotated. The SNP (C-to-T) mutation resulted in premature termination of the protein. Thus, genetic and genomic analysis pinpointed that the truncated F-Box Protein Csa4P641640 that was responsible for the dwarf phenotype in 406M.