|Baldwin, Ransom - Randy|
|Blomberg, Le Ann|
|Wu, Sitao - University Of California|
|Li, Weizhong - University Of California|
Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/18/2015
Publication Date: 1/21/2016
Citation: Li, C., Li, R.W., Baldwin, R.L., Blomberg, L., Wu, S., Li, W. 2016. Transcriptomic sequencing reveals a set of unique genes activated by butyrate-induced histone modification. Scientific Reports. 10:1-8.
Interpretive Summary: Butyrate is a nutritional element with strong epigenetic regulatory activity. Using next generation sequencing, a set of genes activated by butyrate treatment was detected. Their biological functions and gene networks were analyzed using web based software. The results can accelerate our understanding of the molecular mechanisms underlying butyrate-induced epigenomic regulation.
Technical Abstract: Butyrate is a nutritional element with strong epigenetic regulatory activity as an inhibitor of histone deacetylases (HDACs). Based on the analysis of differentially expressed genes induced by butyrate in the bovine epithelial cell using deep RNA-sequencing technology (RNA-seq), a set of unique genes activated only after butyrate treatment was revealed. A complementary bioinformatics analysis of the functional category, pathway and integrated network using Ingenuity Pathways Analysis (IPA) indicated that these butyrate-induced genes are related to predominant cellular processes affected by butyrate cell morphological changes, cell cycle arrest, and apoptosis. Our results provid insight into the alterations induced by butyrate, and accelerat our understanding of the molecular mechanisms underlying butyrate-induced epigenomic regulation.