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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #316430

Research Project: BIOCONTROL AGENT PRODUCTION AND DEPLOYMENT TECHNOLOGIES FOR THE INTEGRATED MANAGEMENT OF PLANT PATHOGENS

Location: Crop Bioprotection Research

Title: Bacillus glycinifermentans sp. nov., isolated from fermented soybean paste

Author
item Kim, Soo-jin - National Academy Of Agricultural Science
item Dunlap, Christopher
item Kwon, Soon-wo - National Academy Of Agricultural Science
item Rooney, Alejandro - Alex

Submitted to: International Journal of Systematic and Evolutionary Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/13/2015
Publication Date: 10/1/2015
Publication URL: http://handle.nal.usda.gov/10113/62295
Citation: Kim, S.J., Dunlap, C.A., Kwon, S.W., Rooney, A.P. 2015. Bacillus glycinifermentans sp. nov., isolated from fermented soybean paste. International Journal of Systematic and Evolutionary Microbiology. 65(10):3586-3590.

Interpretive Summary: This study describes a new bacterial species that was isolated from fermented soybean paste. Novel bacteria may possess unique and unusual biochemical properties that make them attractive candidates to explore their potential for new uses in agriculture or biotechnology. As such, this strain was accessioned into the Crop Bioprotection Research Unit’s culture collection where its potential for agricultural applications can be assessed in future studies.

Technical Abstract: Two independent isolates of a Gram-positive, aerobic, motile rod-shaped bacterium were recovered from soybean-based fermented foodstuffs. Two were isolated from cheonggukjang, a Korean fermented soybean food product. Multilocus sequencing analysis of the 16S rRNA gene and 5 protein coding genes indicated that these strains were most closely related to Bacillus sonorensis KCTC-13918**T and Bacillus licheniformis DSM 13**T. In phenotypic characterization, the novel strains were found to grow between 15-55 'C and to tolerate up to 10% NaCl (w/v). Furthermore, the strains grew in media with pH 5 to 10 (optimal growth at pH 7.0–8.0). The predominant cellular fatty acids were anteiso-C15':'0 (42.35'%) and iso-C15':'0 (22.65'%). The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unknown glycolipid. The DNA G+C was 45.9 mol%. In addition, to forming a distinct monophyletic cluster in phylogenetic analysis of gene sequence data, the strains differed from the two most closely related species in that they did not metabolize D-maltose, D-trehalose, D-galactose or D-sorbitol. Based upon the consensus of phylogenetic and phenotypic analyses, these strains represent a novel species within the genus Bacillus, for which the name Bacillus glycinifermentans sp. nov. is proposed.