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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #315435

Research Project: Resources for the Genetic Improvement of Potato

Location: Vegetable Crops Research

Title: Colonization of wild potato plants by Streptomyces scabies

Author
item Allen, Christina - University Of Wisconsin
item Jansky, Shelley
item Charkowski, Amy - University Of Wisconsin

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 6/4/2015
Publication Date: 8/1/2015
Citation: Allen, C., Jansky, S.H., Charkowski, A.O. 2015. Colonization of wild potato plants by Streptomyces scabies [abstract]. American Phytopathological Society. Paper No. 25-P.

Interpretive Summary:

Technical Abstract: The bacterial pathogen Streptomyces scabies produces lesions on potato tubers, reducing their marketability and profitability. M6 and 524-8 are two closely related inbred diploid lines of the wild potato species Solanum chacoense. After testing in both field and greenhouse assays, it was found that M6 is highly susceptible to common scab while 524-8 is highly resistant. Little is known about genetic or physiological mechanisms of resistance or how S. scabies grows on potato plants. EGFP-labeled S. scabies was used to examine the areas of the potato plant that were colonized, whether S. scabies colonization differs on resistant and susceptible plants, and how colonization changes over time. To accomplish, this tubers of M6 and 524-8 were planted in potting mix inoculated with GFP-labeled S. scabies. Plants were destructively harvested and fluorescence microscopy was used to study colonization of plant tissues by S. scabies.