|KIM, M - Former ARS Employee|
|Bono, James - Jim|
|Kalchayanand, Norasak - Nor|
|BENSON, A - University Of Nebraska|
|Wells, James - Jim|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/10/2015
Publication Date: 4/13/2015
Citation: Kim, M., Kuehn, L.A., Bono, J.L., Berry, E.D., Kalchayanand, N., Freetly, H.C., Benson, A.K., Wells, J. 2015. The effect of bovine fecal microbiome on Escherichia coli O157:H7 prevalence [abstract]. 2015 Congress on Gastrointestinal Function, 2015 April 13-15, Chicago, IL. p. 34.
Technical Abstract: The objective of this study was to determine if fecal microbiome would have an association on E. coli O157:H7 prevalence. Pyrosequencing analysis of fecal microbiome was performed from feedlot cattle fed one of three diets: i) 94 heifers fed low concentrate (LC) diet, ii) 142 steers fed moderate concentrate (MC) diet, and iii) 132 steers fed high concentrate (HC) diet. A total of 2,411,122 high-quality sequences were obtained from 368 samples. In the LC diet group, 402,080 of the total sequences were recovered from 94 fecal samples and classified to 29 phyla. Firmicutes, candidate division TM7, Bacteroidetes, Tenericutes, Proteobacteria, Verrucomicrobia and Actinobacteria were core measurable phyla detected across almost all the 94 fecal samples. In the MC diet group, 1,035,186 of the total sequences were recovered from 142 fecal samples and classified to 22 phyla. Firmicutes, Bacteroidetes, Proteobacteria, Tenericutes, TM7, Cyanobacteria and Actinobacteria were core measurable phyla detected across almost all the 142 samples. In the HC group, 973,856 of the total sequences were recovered from 132 fecal samples and assigned to 26 phyla. Firmicutes, Bacteroidetes, Tenericutes, Proteobacteria, Actinobacteria, Cyanobacteria and TM7 were core measurable phyla detected across almost all the 132 samples. The 2,411,122 sequences were clustered into 322,585 OTUs at 97% sequence similarity. Stepwise regression with backward elimination indicated that 6, 15 and 10 OTUs brought significant information to each model (P < 0.0001) and explained 29, 51 and 33% of E. coli O157:H7 prevalence for each diet group, respectively. Research efforts would need to be made to isolate and characterize species corresponding to these OTUs correlated with E. coli O157:H7 prevalence.