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ARS Home » Southeast Area » Mississippi State, Mississippi » Crop Science Research Laboratory » Genetics and Sustainable Agriculture Research » Research » Publications at this Location » Publication #309855

Research Project: Genetic Enhancement of Cotton by Marker-Assisted and Conventional Breeding, and Introgression of Genes from Exotic Gossypium Species

Location: Genetics and Sustainable Agriculture Research

Title: Molecular confirmation of Gossypium hirsutum chromosome substitution lines

Author
item Saha, Sukumar
item Stelly, David - Texas A&M University
item Makamov, Abdusalom - Uzbekistan Academy Of Sciences
item Ayubov, Mirzakamol - Uzbekistan Academy Of Sciences
item Raska, Dwaine - Texas A&M University
item Gutierrez, Osman
item Manchali, Shivapriya - Gulgarga University
item Jenkins, Johnie
item Deng, Dewayne
item Abdurakhmonov, Ibrokhim - Uzbekistan Academy Of Sciences

Submitted to: Genetica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/21/2015
Publication Date: 5/7/2016
Citation: Saha, S., Stelly, D.M., Makamov, A.K., Ayubov, M., Raska, D., Gutierrez, O.A., Manchali, S., Jenkins, J.N., Deng, D.D., Abdurakhmonov, I.Y. 2016. Molecular confirmation of Gossypium hirsutum chromosome substitution lines. Genetica. 144(3):289-306.

Interpretive Summary: The narrow genetic base of Upland cotton cultivars, Gossypium hirsutum L. was one of the major constraints contributing to the recent declines in fiber yield and quality. Wild species and unadapted germplasms are useful genetic resources that can be tapped to broaden the germplasm base. However, conventional methods of interspecific introgression to improve Upland cotton was not very successful due to technical and biological challenges. To help overcome barriers to effective introgression, we have developed a number of alien chromosome substitution (CS) lines from G. barbadense, G. mustellinum, and G. tomentosum, most of which are nearly isogenic to the inbred ‘Texas Marker-1’ (TM-1), a genetic standard. At the time CS line development was initiated, molecular markers did not exist for some CS lines, and most of these lines were developed based on cytological analysis without using any marker-based testing. The specific objective of this paper is to report on the genetic identity of the CS lines using SSR markers and some special characteristics associated with some of the CS lines. We used chromosome specific SSR markers following standard methods of DNA extraction, PCR and ABI Genetic Analyzer 3130xl to confirm the identity of the CS lines. For most CS lines, the observed SSR profiles were concordant with expectations as per the results of cytological analysis. For a minority of markers and lines, however, the results were discordant; these markers, linkage groups, and CS lines will be further investigated to understand and define their genetic identity for use as breeding resources.

Technical Abstract: The primary gene pool for tetraploid cotton species includes G. hirsutum L., as well as the other four 2n=52 species of Gossypium (G. barbadense, G. mustellinum, G. tomentosum and G. darwinii). To help overcome barriers to effective introgression, we have developed a number of alien chromosome substitution (CS) lines from G. barbadense, G. mustellinum and G. tomentosum, most of which are nearly isogenic to the inbred ‘Texas Marker-1’ (TM-1), a genetic standard. At the time CS line development was initiated, molecular markers did not exist for some CS lines, and most of these lines were developed based on cytological analysis without using any marker-based testing. Here, we report on tests with SSR markers specific to the substituted chromosome or chromosome segment from one or more linkage maps to assess the constitution and genetic identity of the CS lines. The specific objective of this paper is to report on the genetic identity of the CS lines using SSR markers and some special characteristics associated with some of the CS lines. We used chromosome specific SSR markers following standard methods of DNA extraction, PCR and according to manufacturer’s protocol on ABI Genetic Analyzer 3130xl to confirm the identity of the introgressed alien chromosome or chromosome segments in the CS lines. For most CS lines and most mapped markers, the observed SSR profiles were concordant with expectations as per the results of cytological analysis. For a minority of markers and lines, however, the results were discordant; these markers, linkage groups, and CS lines will be further investigated to understand and define their genetic identity for use as breeding resources.