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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Crop Bioprotection Research » Research » Publications at this Location » Publication #308675

Research Project: PRODUCTION, STABILIZATION AND FORMULATION OF MICROBIAL AGENTS AND THEIR NATURAL PRODUCTS

Location: Crop Bioprotection Research

Title: Maximizing in vivo production of Agrotis ipsilon (Hufnagel) baculovirus

Author
item Behle, Robert

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 11/19/2014
Publication Date: 11/19/2014
Citation: Behle, R.W. 2014. Maximizing in vivo production of Agrotis ipsilon (Hufnagel) baculovirus [abstract]. Entomological Society of America. Poster D3485.

Interpretive Summary:

Technical Abstract: The black cutworm, Agrotis ipsilon (Hufnagel), is a pest causing damage to a variety plants of from turf to row crops. A recently discovered baculovirus has the potential to be developed as a biological pesticide to provide targeted control of this insect pest. Initial field trials in turf grass and corn using this baculovirus for the control of the cutworm reduced plant damage. In an effort to progress this baculovirus as a biological pesticide, experiments were conducted to determine parameters necessary for a cost effective in vivo production system using cutworm larvae. Three larval diets, larval age at infection (7-10 d-old) and dosage of virus exposure (1 x 105 to 1 x 108 occlusion bodies ml-1) were evaluated. Generally speaking, diets that maximized larval growth resulted in greater virus production. Higher dosages for larval exposure were necessary to infect older/larger larvae, and resulted in greater virus yields. These results will support the development of this baculovirus as an additional tool for the integrated control of the black cutworm.