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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Animal Genomics and Improvement Laboratory » Research » Publications at this Location » Publication #302845


Location: Animal Genomics and Improvement Laboratory

Title: Linkage disequilibrium levels in Bos indicus and Bos taurus cattle using medium and high density SNP chip data and different minor allele frequency distributions

item Perez O'brien, Ana
item Meszaros, Gabor
item Utsunomiya, Yuri
item Sonstegard, Tad
item Fernando Garcia, Jose
item Van Tassell, Curtis - Curt
item Carvalheiro, Roberto
item Da Silva, Marco
item Solkner, Johann

Submitted to: Livestock Science
Publication Type: Book / Chapter
Publication Acceptance Date: 5/14/2014
Publication Date: 5/22/2014
Citation: Perez O'Brien, A.M., Meszaros, G., Utsunomiya, Y.T., Sonstegard, T.S., Fernando Garcia, J., Van Tassell, C.P., Carvalheiro, R., Da Silva, M.V., Solkner, J. 2014. Linkage disequilibrium levels in Bos indicus and Bos taurus cattle using medium and high density SNP chip data and different minor allele frequency distributions. Livestock Science. 166:121-132.

Interpretive Summary:

Technical Abstract: Linkage disequilibrium (LD), the observed correlation between alleles at different loci in the genome, is a determinant parameter in many applications of molecular genetics. With the wider use of genomic technologies in animal breeding and animal genetics, it is worthwhile revising and improving the current knowledge and understanding of cattle LD. This study analyzes levels of LD assessed through the r² measurement in seven breeds of cattle from both indicine (Bos indicus) and taurine (Bos taurus) (sub)species, genotyped with a high density panel (HD) of over 777000 single nucleotide polymorphism (SNP) markers. Average levels of LD were assessed for inter-marker distances from 0Kb up to 10Mb. Two more SNP panels were built in silico from the original genotypes to represent a lower density SNP chip including approximately 50000 SNPs (50K) and a panel of 249000 SNPs chosen to comply with specific minor allele frequency (MAF) distributions. The results show higher levels of LD than previously reported, especially at short inter-marker distances of less than 20Kb, assessed through the use of the higher density panel. Negligible impact of MAF changes in the estimation of r² were observed, supporting the use of the HD panel for LD-based methodologies even when ascertainment bias in the choice of SNPs is suspected. The 50K SNP panel indicated lower levels of LD in the first 100Kb and highly unreliable LD estimates for distances shorter than 50Kb. Overall, lower levels of LD were observed for all indicine breeds as compared to taurine breeds, with larger differences assessed in the first 100Kb, suggesting the need for use of higher SNP densities in this (sub)species when applying LD-dependent methods, as compared to most taurine breeds.