Submitted to: Clinical and Vaccine Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/1/2013
Publication Date: 6/1/2013
Publication URL: http://handle.nal.usda.gov/10113/61366
Citation: Register, K.B., Sacco, R.E., Olsen, S.C. 2013. Evaluation of enzyme-linked immunosorbent assays for detection of Mycoplasma bovis-Specific antibody in bison sera. Clinical and Vaccine Immunology. 20(9):1405-1409 DOI: 10.1128/CVI.00409-13. Interpretive Summary: Mycoplasma bovis has recently emerged as a significant health threat in bison and is an increasing concern and source of economic loss for producers. A tool for serological detection of infected bison is critical for establishing the prevalence and transmission patterns of M. bovis. Standardized ELISA kits validated for detection of seropositive cattle are commercially available, but their suitability for use with bison sera has not been evaluated. In this study, sera from bison infected or vaccinated with M. bovis were used to assess the performance of two commercially available ELISAs intended for use in cattle. The data presented suggest they are not optimal for identification of seropositive bison, particularly those with low to moderate levels of antibody. A newly developed ELISA is described that provides a good starting point for an improved and standardized enzyme immunoassay. Additional optimization and analysis must be conducted before it can be recommended for general use or validated for clinical serodiagnosis.
Technical Abstract: Mycoplasma bovis has recently emerged as a significant and costly infectious disease problem in bison. This report demonstrates that ELISAs for detection of M. bovis-specific antibody in cattle are not optimal for identification of seropositive bison. An ELISA optimized for use with bison sera is described.