Submitted to: NIH Gene Expression Omnibus (GEO) Database
Publication Type: Other
Publication Acceptance Date: 7/8/2013
Publication Date: 2/24/2014
Citation: Li, C., Li, R.W. 2014. ChIp-seq of bovine cells (MDBK) to study butyrate-induced histone modification with 10 datasets. NIH Gene Expression Omnibus Respository: (GEO Accession GSE38973). Interpretive Summary:
Technical Abstract: Next-generation sequencing was combined with chromatin immunoprecipitation (ChIP) technology to analyze histone modification (acetylation) induced by butyrate and to map the epigenomic landscape of normal histone H3, H4 in rumen cells of the cow. Ten variants of histone H3 and H4 modification were mapped on a whole-genome scale (acetyl-H3K18-ChIP-seq; trimethyl-H3K9 ChIP-seq; Histone H4 ChIP-seq; acetyl-H4K5 ChIP-seq; acetyl-H4K12 ChIP-seq;acetyl-H4K16 ChIP-seq; Histone H3 ChIP-seq; Acetyl H3H9 ChIP-seq; acetyl H3K27 ChIP-seq; and tetra-acetyl H4 ChIP-seq). These datasets illustrate epigenomic landscapes modified by butyrate-induced histone acetylation. This study is a crucial starting point for evaluating mechanisms involved in rumen epithelial epigenomic regulation in the cow.