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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT OF GENOMIC TOOLS TO STUDY RUMINANT RESISTANCE TO GASTROINTESTINAL NEMATODES

Location: Animal Genomics and Improvement Laboratory

Title: Identification of blaOXA-51, blaOXA-58, blaDIM-1 and blaVIM carbapenemase genes in hospital enterobacteriaceae isolates from Sierra Leone)

Author
item Leski, Tomasz
item Bangura, Umaru
item Jimmy, David
item Ansumana, Rashid
item Lizewski, Stephen
item Li, Robert
item Stenger, David
item Taitt, Chris
item Vora, Gary

Submitted to: Journal of Clinical Microbiology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 5/2/2013
Publication Date: 5/8/2013
Citation: Leski, T., Bangura, U., Jimmy, D.H., Ansumana, R., Lizewski, S.E., Li, R.W., Stenger, D.A., Taitt, C.R., Vora, G. 2013. Identification of blaOXA-51, blaOXA-58, blaDIM-1 and blaVIM carbapenemase genes in hospital enterobacteriaceae isolates from Sierra Leone. Journal of Clinical Microbiology. 51:2435-2438.

Interpretive Summary: The gastrointestinal microbiome acts as a metabolic organ and plays a critical role in host nutrient biosynthesis and utilization, development of the host immune system and diseases in vertebrates. Carbapenem antibiotics are currently the most potent ß-lactam antibiotics clinically available and are used as a last resort to treat infections caused by multidrug resistant Gram-negative pathogens. Carbapenemases are frequently found in pathogenic bacterial strains that confer resistance to various antibiotics, such as aminoglycosides, tetracyclines, and ß-lactams. In this report, we identified 15 carbapenemase genes. Our findings enable a better surveillance of antimicrobial resistance determinants to better understand the scope, severity and evolution of multidrug resistance among microbes from around the world.

Technical Abstract: We describe the results of a molecular epidemiological survey of 15 carbapenemase-encoding genes from a recent collection of clinical isolates. The most salient findings revealed that (i) 60% of the isolates harbored multiple carbapenemase genes, (ii) the blaDIM-1 gene that has only been reported in the Netherlands is also circulating in this environment and (iii) the blaOXA-51-like and blaOXA-58 genes, which were thought to reside exclusively in Acinetobacter species, can also be found in members of the Enterobacteriaceae.

Last Modified: 8/24/2016
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