|Dreesen, Leentje - Ghent University|
|Rinaldi, Minuela - Ghent University|
|Claerbout, Edwin - Ghent University|
|Geldhof, Peter - Ghent University|
Submitted to: NIH Gene Expression Omnibus (GEO) Database
Publication Type: Other
Publication Acceptance Date: 6/17/2012
Publication Date: 6/27/2012
Citation: Li, R.W., Dreesen, L., Rinaldi, M., Claerbout, E., Geldhof, P. 2012. The transcriptomic profiles of Giardia duodenalis infected calves. NIH Gene Expression Omnibus (GEO) Database. GSE35920.
Technical Abstract: Giardia duodenalis is one of the most commonly found intestinal pathogens in humans and animals. However, little is known about the host-parasite interaction in its natural hosts. The objective of this study was to investigate the intestinal response in calves following a G. duodenalis infection, using a bovine high-density oligo microarray to analyze global gene expression in the small intestine. Our microarray data results suggest a decrease in inflammation, immune response, and immune cell migration in infected animals, which was examined in more detail by quantitative real-time PCR on a panel of cytokines combined with histological analyses. The cytokine transcription levels showed a trend of down regulated expression in infected animals compared to the negative controls, best seen in the jejunum for IL-6 and IL-8 and statistically significant for IL-17, IL-13 and IFN-'. No increased immune cell recruitment could be seen after infection, as well as no intestinal pathologies, such as villi shortening or increased levels of apoptosis. Key regulators in this intestinal response seem to be the nuclear peroxisome proliferator-activated receptors alpha (PPARA) and gamma (PPARG), for which an up-regulated expression was seen in microarray and qRT-PCR data. The activation of PPARs can exert an anti-inflammatory effect with inhibition of pro-inflammatory cytokines and a decrease in cell recruitment. How the PPARs are activated during a Giardia infection still needs to be further elucidated.