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United States Department of Agriculture

Agricultural Research Service

Research Project: Biologically-based Technologies for Management of Crop Insect Pests in Local and Areawide Programs

Location: Insect Behavior and Biocontrol Research

Title: Identification and analysis of the major yolk polypeptide from the caribbean fruit fly, Anastrepha suspensa (Loew))

Author
item Handler, Alfred - Al
item Shirk, Paul

Submitted to: Archives of Insect Biochemistry and Physiology
Publication Type: Peer reviewed journal
Publication Acceptance Date: 7/11/1988
Publication Date: N/A
Citation: Handler, A.M., Galette, A.S. 1988. Identification and analysis of the major yolk polypeptide from the caribbean fruit fly, Anastrepha suspensa (Loew). Archives of Insect Biochemistry and Physiology. 9:91-106.

Interpretive Summary: A single major yolk polypeptide (YP) having a molecular mass of approximately 48,000 daltons (Da), was identified in the ovaries and oviposited eggs of the Caribbean fruit fly by scientists at the USDA Agricultural Research Service, Center for Medical Agricultural and Veterinary Entomology, Gainesville, Florida. The polypeptide was partially purified from oviposited eggs using gel permeation and ion-exchange chromatography. Analysis of YP synthesis in vivo and in tissues cultured in vitro indicated that the ovary was the major site of synthesis with very low levels of YP derived from the adult fat body. Using a monospecific polyclonal antiserum to 48 kDa YP in an immunoblot assay, low levels of vitellogenin were found in female hemolymph; slightly lower levels of an immunoreactive 48-kDa polypeptide were detectable in male hemolymph. Although YP synthesis was detectable within 12 h after eclosion, the major increase in YP accumulation occurred at 3–4 days posteclosion coincident with the initiation of observable yolk deposition. The physical characteristics of YP from A. suspensa were similar to YPs from other dipterans in terms of molecular mass and antigenicity, yet the tissue- and sex-specific regulation of the YP differed from other dipterans as well as most other insects.

Technical Abstract: A single major yolk polypeptide (YP) having a molecular mass of approximately 48,000 daltons (Da), was identified in the ovaries and oviposited eggs of the Caribbean fruit fly, Anastrepha suspensa. The polypeptide was partially purified from oviposited eggs using gel permeation and ion-exchange chromatography. Analysis of YP synthesis in vivo and in tissues cultured in vitro indicated that the ovary was the major site of synthesis with very low levels of YP derived from the adult fat body. Using a monospecific polyclonal antiserum to 48 kDa YP in an immunoblot assay, low levels of vitellogenin were found in female hemolymph; slightly lower levels of an immunoreactive 48-kDa polypeptide were detectable in male hemolymph. Although YP synthesis was detectable within 12 h after eclosion, the major increase in YP accumulation occurred at 3–4 days posteclosion coincident with the initiation of observable yolk deposition. The physical characteristics of YP from A. suspensa were similar to YPs from other dipterans in terms of molecular mass and antigenicity, yet the tissue- and sex-specific regulation of the YP differed from other dipterans as well as most other insects.

Last Modified: 8/24/2016
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