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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Food Safety and Intervention Technologies Research » Research » Publications at this Location » Publication #277568

Title: Inactivation of foodborne pathogens on crawfish tail meat using cryogenic freezing and gamma radiation

item Rajkowski, Kathleen
item Sommers, Christopher
item ANTENUCCI, RACHEL - Former ARS Employee
item Scullen, Butch - Butch
item Cassidy, Jennifer
item BENDER, ERIC - Air Products & Chemicals

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/22/2012
Publication Date: 7/22/2012
Citation: Rajkowski, K.T., Sommers, C.H., Antenucci, R., Scullen, O.J., Cassidy, J.M., Bender, E. 2012. Inactivation of foodborne pathogens on crawfish tail meat using cryogenic freezing and gamma radiation [abstract]. IAFP Meeting. July 22-25, 2012, Providence, Rhode Island. 1:1.

Interpretive Summary:

Technical Abstract: Foodborne illness outbreaks occasionally occur as a result of microbiologically contaminated crustaceans, including crawfish. Cryogenic freezing and gamma radiation are two technologies which can be used to improve the microbiological safety and shelf-life of foods. In the U.S. the majority of non-canned seafood is either frozen or previously frozen. A petition to allow irradiation of crustaceans is currently being evaluated by the U.S. Food and Drug Administration. There is little data on the use of commercial or pilot scale equipment and processes to quantify the effect of freezing on foodborne pathogen survival. The purpose of this study is to evaluate the effect of cryogenic freezing and gamma radiation on the survival of foodborne pathogens in crawfish tail meat. In this study the use of cryogenic freezing (CF) and gamma radiation (GR) were evaluated for their ability to inactivate the foodborne pathogens E. coli O157:H7, Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, and Vibrio spp. inoculated on partially cooked crawfish tail meat. The final inoculums level on the crawfish tail meat was 6 – 7 log CFU/g. CF (-59 deg C, 3 min) resulted in 0.85, 0.71, and 0.80 log10 reductions of the gram negative pathogens E. coli O157:H7, Salmonella spp., and Vibrio spp., respectively, following freezing and one week storage at -20deg C. CF had no effect on the viability of L. monocytogenes or S. aureus (gram positive). D10 values (the radiation dose needed to inactivate one log10 of microorganism) of the foodborne pathogens which were irradiated following one week frozen storage were 0.81, 0.58, 0.50, 0.49, and 0.36 kGy for L. monocytogenes, S. aureus, Vibrio spp., Salmonella spp., and E. coli O157:H7, respectively. The radiation D10 of background microflora in non-inoculated frozen tail meat was 1.58 and 1.64 kGy for mesophilic and psychrotrophic bacteria, respectively. This research provides the seafood and radiation processing industries the necessary information to inactivate foodborne pathogens in crawfish tail meat.