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ARS Home » Midwest Area » St. Paul, Minnesota » Soil and Water Management Research » Research » Publications at this Location » Publication #259759

Title: Analysis of Turf Fungicides in Snow Melt Runoff by LC/MS

item Hamlin, Jennifer
item BARBER, BRIAN - University Of Minnesota
item Rice, Pamela
item Koskinen, William

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/2010
Publication Date: 10/16/2010
Citation: Rittenhouse, J.L., Barber, B.B., Rice, P.J., Koskinen, W.C. 2010. Analysis of Turf Fungicides in Snow Melt Runoff by LC/MS [abstract]. ASA-CSSA-SSSA Annual Meeting Abstracts. Available:

Interpretive Summary:

Technical Abstract: Fungicides are applied on turf grass, in autumn, to control snow mold in the north-central United States. Fungicides of varying chemical classes have been detected in snow melt runoff from turf. A multi-residue method for simultaneous sample extraction and analysis is needed to process a large quantity of snow melt samples. A method was developed to extract three fungicides, quintozene, iprodione, and chlorothalonil, in addition to its major degradation product 4-hydroxychlorothalonil simultaneously. Water samples were spiked with these four compounds, plus the herbicide acetochlor as a surrogate, and then extracted using a C18 solid phase extraction (SPE) cartridge. The extracts were divided by volume then analyzed by gas chromatography-mass spectrometry (GC/MS) or liquid chromatography-mass spectrometry (LC/MS). The herbicide metazachlor was used as the internal standard for GC/MS analysis of quintozene and chlorothalonil. The insecticide isofenphos was the internal standard for LC/MS analysis of 4-hydroxychlorothalonil and iprodione. The surrogate, acetochlor was analyzed by both instruments. Extraction recoveries for the compounds of interest ranged from 44 to 94%. Limits of detection were below 10 ppb for chlorothalonil and 4-hydroxychlorothalonil and below 25 ppb for iprodione and quintoszene.