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United States Department of Agriculture

Agricultural Research Service

Title: Comparative Genomics Reveals Mobile Pathogenicity Chromosomes in Fusarium)

Author
item Ma, Li-jun
item Borkovich, Katherine
item Coleman, Jeffrey
item Daboussi, Marie-josee
item Pietro, Antonio
item Dufresne, Marie
item Freitag, Michael
item Grabherr, Manfred
item Henrissat, Bernard
item Kang, Seogchan
item Park, Jongsun
item Rep, Martijn
item Shim, Won-bo
item Woloshuk, Charles
item Xie, Xiaohui
item Xu, Jin-rong
item Antoniw, John
item Baker, Scott
item Bluhm, Burton
item Breakspear, Andrew
item Brown, Daren
item Butchko, Robert
item Chapman, Sinead
item Coulson, Richard
item Coutinho, Pedro
item Danchin, Etienne g.j.
item Diener, Andrew
item Gale, Liane
item Gardiner, Donald
item Goff, Stephen
item Hammond-kosack, Kim
item Hilburn, Karen
item Hua-van, Aurelie
item Jonkers, Wilfried
item Kazan, Kemal
item Kodira, Chinnappa
item Koehrsen, Michael
item Kumar, Lokesh
item Lee, Yong-hwan
item Li, Liande
item Manners, John
item Miranda-saavedra, Diego
item Mukherjee, Mala
item Park, Gyungsoon
item Park, Jongsun
item Park, Sook-young
item Proctor, Robert
item Regev, Aviv
item Ruiz-roldan, M. carmen
item Sain, Divya
item Sakthikumar, Sharadha
item Sykes, Sean
item Schwartz, David
item Turgeon, Gillian
item Wapinski, Ilan
item Yoder, Olen
item Young, Sarah
item Zeng, Qiandong
item Zhou, Siguo
item Galagan, James
item Cuomo, Christina
item Kistler, H - Corby

Submitted to: Nature
Publication Type: Peer reviewed journal
Publication Acceptance Date: 1/20/2010
Publication Date: 3/18/2010
Citation: Ma, L., Borkovich, K.A., Coleman, J.J., Daboussi, M., Pietro, A.D., Dufresne, M., Freitag, M., Grabherr, M., Henrissat, B., Kang, S., Park, J., Rep, M., Shim, W., Woloshuk, C., Xie, X., Xu, J., Antoniw, J., Baker, S.E., Bluhm, B.H., Breakspear, A., Brown, D.W., Butchko, R.A., Chapman, S., Coulson, R., Coutinho, P.M., Danchin, E., Diener, A., Gale, L., Gardiner, D., Goff, S., Hammond-Kosack, K.E., Hilburn, K., Hua-Van, A., Jonkers, W., Kazan, K., Kodira, C.D., Koehrsen, M., Kumar, L., Lee, Y., Li, L., Manners, J., Miranda-Saavedra, D., Mukherjee, M., Park, G., Park, J., Park, S., Proctor, R., Regev, A., Ruiz-Roldan, M., Sain, D., Sakthikumar, S., Sykes, S., Schwartz, D.C., Turgeon, G., Wapinski, I., Yoder, O., Young, S., Zeng, Q., Zhou, S., Galagan, J., Cuomo, C.A., Kistler, H.C. 2010. Comparative Genomics Reveals Mobile Pathogenicity Chromosomes in Fusarium. Nature. 464(7287):367-373.

Interpretive Summary: The results of this research demonstrate differences in genome sequences that affect toxin production and ability to cause plant disease in the fungus Fusarium. Species of this fungus are among the most economically important fungal pathogens of crop plants because they occur worldwide and on many crops, they can reduce crop yield and quality, and they can produce toxins (mycotoxins) that are hazards to human and animal health. Here, the whole genome sequence was determined for two Fusarium species, one that causes disease on tomato and a second that causes disease on corn. The sequences were compared with a previously determined whole genome sequence of a third species that causes disease on wheat, corn, and barley. The results demonstrate that the genome sequences are highly similar in the three species, but that important differences exist. Some of the genes that differ among the three species are required for production of different toxic metabolites, whereas others are important for ability to cause disease on crop hosts. These findings provide information that plant breeders and plant biotechnologists can use to improve the resistance of crops to diseases caused by Fusarium and to reduce mycotoxin contamination of crops.

Technical Abstract: Fusarium species are among the most important phytopathogenic and toxigenic fungi, having significant impact on crop production and animal health. Distinctively, strains of F. oxysporum exhibit wide host range and are pathogenic to both plant and animal species, reflecting remarkable genetic adaptability. To understand the mechanism underlying such genetic plasticity and rapid pathogenic development, we compared the genomes of three economically important and phylogenetically related, yet phenotypically distinct phytopathogenic species, F. graminearum, F. verticillioides and F. oxysporum f. sp. lycopersici. Comparative analysis revealed diverse and actively transcribed secondary metabolite biosynthetic clusters in F. graminearum and F. verticillioides as well as greatly expanded lineage-specific (LS) genomic regions in F. oxysporum that include four entire chromosomes and account for more than one-quarter of the genome. LS regions are rich in transposons and genes involved in host-pathogen interactions, including known effectors, enzymes targeting plant substrates or processes, and genes involved in lipid signalling and gene silencing. We found evidence for the acquisition of the LS chromosomes through horizontal transfer, which may explain the polyphyletic origin of host specificity in F. oxysporum and the rapid emergence of new pathogenic lineages in distinct genetic backgrounds.

Last Modified: 8/24/2016
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