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United States Department of Agriculture

Agricultural Research Service

Title: DESI-MS Analysis of Mycotoxins from Grain Matrices

item Busman, Mark

Submitted to: American Society for Mass Spectrometry
Publication Type: Abstract Only
Publication Acceptance Date: 6/5/2008
Publication Date: 6/5/2008
Citation: Busman, M. 2008. DESI-MS Analysis of Mycotoxins from Grain Matrices. American Society for Mass Spectrometry. Poster No. WP074. . American Society for Mass Spectrometry.

Interpretive Summary: A new mass spectrometry method is demonstrated for the analysis of the toxins produced by fungi in grain. The new method will require minimal sample preparation prior to instrumental analysis. The method should benefit grain processors by providing a rapid method for reliable toxin analysis without expert trained personnel for processing the grain matrix prior to analysis.

Technical Abstract: Introduction – Determination of mycotoxins in grain matrices is a frequent need of growers and processors of cereal grains. Sample preparation for mycotoxin analysis is often laborious and time consuming. Here we demonstrate the application of desorption electrospray ionization – mass spectrometry (DESI-MS) to the analysis of mycotoxins from grain. Method – A DESI source was constructed for interface to a ThermoFinnigan LCQ – DECA ion trap mass spectrometer. Mycotoxin (fumonisin B1, fusaric acid, deoxynivalenol) standard and grain samples were applied to a variety of sample plate types for DESI-MS. Grain samples were subjected to a variety of sample preparation protocols. The sample preparation for grain ranged from traditional grinding followed by extraction to a minimal technique where intact kernels were directly analyzed. Preliminary data – A DESI source was fitted to a ThermoFinnigan LCQ – DECA ion trap mass spectrometer. A variety of mycotoxin standards were spotted on glass, PMMA and paper substrates. Results were compared with those from flow injection electrospray ionization (ESI) – mass spectrometry. Grain samples were analyzed for various mycotoxins by DESI-MS. Intact kernels were analyzed by DESI-MS from glass substrates. Results of the DESI-MS were compared with ESI-MS, utilizing the established LC-ESI-MS methods for each of the mycotoxins. Applicability of the DESI-MS technique for sorting of intact grain kernels on the basis of toxin content is demonstrated. Novel Aspect – A DESI-MS interface is utilized for evaluation of mycotoxins from grain matrices.

Last Modified: 10/15/2017
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