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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #210437
Title: Genetic Diversity of Tropical Isolates and Color Variants of Aureobasidium pullulans

Author
item MANITCHOTPISIT, P - CHULALONGKORN UNIV
item Leathers, Timothy
item Li, Xin Liang
item Kurtzman, Cletus
item EVELEIGH, D - RUTGERS UNIV
item PRASONGSUK, S - CHULALONGKORN UNIV
item PUNNAPAYAK, H - CHULALONGKORN UNIV

Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 8/2/2007
Publication Date: 8/2/2007
Citation: Manitchotpisit, P., Leathers, T.D., Li, X., Kurtzman, C.P., Eveleigh, D.E., Prasongsuk, S., Punnapayak, H. 2007. Genetic Diversity of Tropical Isolates and Color Variants of Aureobasidium pullulans [abstract]. Society of Industrial Microbiology Annual Meeting. Abstract S116, p. 113.

Interpretive Summary:

Technical Abstract: The polymorphic fungus Aureobasidium pullulans was isolated from many provinces in tropical Thailand and cultured in pullulan production medium (PM) containing sucrose and peptone as carbon and nitrogen sources, respectively. Liquid PM cultures varied in color from cream to pale pink, light burgundy red, light brown, olivaceous green, and black. Most isolates produced colonies that were smooth, moist, and cream or pale pink on yeast malt agar (YMA) medium. Molecular sequence analyses of the internal transcribed spacer (ITS) regions of rDNA showed no differences among these isolates. However, the intergenic spacer (IGS) regions of rDNA revealed the possibility of strain differences. Preliminary data from 9 isolates suggest that IGS-PCR patterns cluster in groups according to color variability. Sequence analysis of 3-4 Kb of IGS1 and IGS2 showed similarities among isolates consistent with the IGS-PCR patterns.