Submitted to: International IUPAC Symposium on Mycotoxins and Phycotoxins
Publication Type: Abstract only
Publication Acceptance Date: 1/9/2007
Publication Date: 5/21/2007
Citation: Maragos, C.M., Appell, M.D., Pascale, M., Lippolis, V., Viscontia, A., Catucci, L. 2007. Cyclodextrins as modifiers of mycotoxin fluorescence [abstract]. International IUPAC Symposium on Mycotoxins and Phycotoxins. Abstract No. 1322. Interpretive Summary:
Technical Abstract: Cyclodextrins, cyclic oligosaccharides composed of amylose subunits, are known to interact with mycotoxins, although the exact stoichiometry and mechanism of the interaction have not been reported. The interactions may be useful to analytical chemists by altering the properties of the mycotoxin of interest, namely the chromatographic properties, fluorescence, or absorption of these fungal metabolites. Practical applications of these effects have been the incorporation of cyclodextrins into the mobile phase of capillary electrophoresis-based separation of the aflatoxins, and the use of cyclodextrins as reagents for improved aflatoxin detection in HPLC. The effects upon fluorescence intensity when added to fungal growth media have meant that cyclodextrins have also found use for detecting mycotoxins in fungal cultures. In addition to their effect upon aflatoxins, the cyclodextrins also influence the native fluorescence of zearalenone (ZEN) and of fluorescently-labeled T-2 toxin. Screening of 22 cyclodextrins revealed several, including heptakis (2,6-di-O-methyl)-beta-cyclodextrin, that substantially enhanced ZEN fluorescence. The latter compound was used as a buffer modifier to increase the sensitivity of a capillary electrophoresis method for detecting ZEN in maize. The cyclodextrins also influence the intensity of non-native fluorescence such as that arising from the labeling of T-2 toxin with pyrene-1-carbonyl cyanide or 1-anthroylnitrile. Because of the multiple effects they produce, the cyclodextrins may make useful additives for a variety of mycotoxin analytical methods, in particular those conducted under aqueous conditions.