Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/12/2006
Publication Date: 10/12/2006
Citation: Schisler, D.A., Boehm, M.J., Dunlap, C.A., Paul, P., Palmquist, D.E. 2006. USDA-ARS and the Ohio State University cooperative research: Use of fractional factorial field designs to assess the integration of diverse treatments against FHB. National Fusarium Head Blight Forum Proceedings. p. 21-26.
Technical Abstract: In previous work, we discovered and patented microbial strains that reduce Fusarium head blight (FHB) of wheat in the greenhouse and field and also demonstrated reduction of FHB via a variety of approaches including the use of UV protectants, fungicide, chemical inducers of systemic acquired disease resistance (SAR), and choline metabolizing microbial antagonists. In this study, these factors were simultaneously tested in field studies using a fractional factorial experimental design. Additionally, food grade dyes were tested for toxicity and ability to protect cells of FHB bacterial antagonist OH 221.3 from the deleterious effects of artificial sunlight in vitro. Of the five dyes tested, naphthol yellow (5 micromolar) was the most efficient in enhancing the survival of cells exposed to six hours of artificial sunlight. Naphthol yellow did not have a deleterious effect on the growth of fresh cells or dried cells not exposed to artificial sunlight. Results from the fractional factorial field studies were dependent on the wheat cultivar considered. On cultivar Elkhart in Wooster, Ohio, the presence of fungicide Folicur 3.6F (P=0.001) and antagonist OH 221.3 (P=0.10) significantly reduced disease severity and incidence. Antagonist OH 182.9 reduced the Deoxynivalenol (DON) content (P=0.04) while naphthol yellow decreased the test weight (P=0.05) of Elkhart grain. SAR chemical sodium salicylic acid (0.01 M) increased DON in Elkhart but reduced DON in Freedom grain (P=0.02). No other treatment component significantly influenced test parameters on Freedom. Analysis of data did not indicate the presence of first order synergistic effects of combining the biocontrol agents, UV protectant, Folicur 3.6F, and SAR chemical. Additional experiments using fractional factorial designs in FHB field studies would be necessary to determine if the design can serve as a useful tool for detecting treatment differences while reducing the amount of field area required.