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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Grain Quality and Structure Research » Research » Publications at this Location » Publication #178617

Title: STUDY OF WHEAT GLUTEN SECONDARY STRUCTURE CONFORMATIONAL CHANGES IN FROZEN DOUGH USING FT-HATR MID-INFRARED SPECTROSCOPY.

Author
item XIE, FENG
item Seabourn, Bradford - Brad
item CHUNG, OKKYUNG
item SEIB, PAUL

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/25/2005
Publication Date: 9/11/2005
Citation: Xie, F., Seabourn, B.W., Chung, O.K., Seib, P.A. 2005. Study of wheat gluten secondary structure conformational changes in frozen dough using FT-HATR Mid-Infrared Spectroscopy.. Abstract No. 245 in: 2005 AACC Annual Meeting Program Book. p.146. Meeting Abstract.

Interpretive Summary:

Technical Abstract: Breadmaking quality of frozen dough is usually inferior to that of freshly mixed dough. A change in gluten structure during freezing and thawing might be one of the reasons that frozen dough has a poorer end-use performance than fresh dough. We investigated changes in wheat gluten secondary structure resulting from freezing and thawing of flour-water “model” doughs optimally mixed, using six hard red winter wheat flours of varying protein content and optimum mix time. Doughs were scanned in the mid-infrared region of the electromagnetic spectrum using Fourier transform horizontal attenuated total reflectance (FT-HATR) mid-infrared spectroscopy. Frozen storage time was 0 min, 24 hr, 1 and 2 weeks, and the thawing time was 45 min for all frozen doughs. The protein secondary structures such as beta-sheet (1242 cm-1-1-1-1-1