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ARS Home » Midwest Area » St. Paul, Minnesota » Soil and Water Management Research » Research » Publications at this Location » Publication #173466
Title: MULTIRESIDUE ANALYSIS OF SEVEN ANTICOAGULANT RODENTICIDES BY LC/ES/MS/MS

Author
item MAREK, LE ETTA - MN DEPT OF AGRIC
item Koskinen, William

Submitted to: American Chemical Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/12/2005
Publication Date: 3/13/2005
Citation: Marek, L.J., Koskinen, W.C. 2005. Multiresidue analysis of seven anticoagulant rodenticides by LC/ES/MS/MS [abstract]. American Chemical Society Abstracts. p. 72.

Interpretive Summary:

Technical Abstract: Mice and rat populations are commonly controlled by two classes of rodenticide anticoagulants, coumarins and indandiones. However, poisoning of nontarget animals also often occurs. For cases such as these, a rapid, multiresidue method, which provides positive confirmation for both classes of anticoagulant rodenticides, is needed by diagnostic laboratories. A method was developed for the determination of seven anticoagulent rodenticides, coumafuryl, pindone,warfarin, diphacinone, chlorophacinone, bromadiolone, and brodifacoum by high performance liquid chromatography/electrospray/mass spectrometry (LC/ES/MS/MS). Separation was performed using a Zorbax RX-C8 (2.1 x 150 mm x 5 ìm) column. The mobile phase gradient elution was 60% 10 mM ammonium acetate: 40% methanol (v:v) from 0 to 4 min, increasing to 50%, to 70% and to 90% methanol at 9, 14, and 18 min, respectively, with a total run time of 24 min. Detection was by MS/MS with electrospray ionization in negative mode. Confirmation was by retention time, m/z of molecular ion and two parent daughter transitions. Recoveries from selected matrices ranged from 70-100%. Detection limits were as low as 1-5 ng g-1. The developed method was rapid and provided the simultaneous quantification and confirmation of the seven anticoagulant rodenticides.