Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/4/2004
Publication Date: 8/4/2004
Citation: Zhang, S., Schisler, D.A., Jackson, M.A., Boehm, M.J., Slininger, P.J. 2004. Cold shock increases air-drying survival of Cryptococcus nodaensis OH 182.9. Phytopathology (American Phytopathological Society Meeting Abstracts). 94(6):S115.
Technical Abstract: Fresh biomass or frozen concentrates of Cryptococcus nodaensis OH 182.9 (NRRL Y-30216) significantly decreased Fusarium head blight in greenhouse and field trials. Fermentation, formulation, and drying studies are necessary and important in order to simplify transportation, storage, and application of this agent. Air-drying is a convenient and economical drying method for developing microbial products. In experiments designed to test the effect of temperature shock during liquid cultivation on survival of OH 182.9 after air-drying, OH 182.9 was grown at various temperatures in semi-defined complete liquid media, with cells grown at 25 deg C for 48 h serving as a control. Harvested cultures were mixed with 5 or 10% diatomaceous earth, vacuum filtered, and air dried for 15-20 h at 60-70% RH. Cell survival at 4 deg C for 14 weeks after air-drying was evaluated. In general, cells grown at 25 deg C for 20 h and then cultivated at 15 deg C for 28 h survived air-drying better than control cells. The survival of cells subjected to heat shock at 31 deg C generally did not differ from control cells regardless of whether heat shock was applied at the late exponential or early stationary stage of growth. In current studies, preliminary data indicate that prolonged (28 h) cold shock at 10 deg C and 15 deg C after incubation at 25 deg C for 20 h increased desiccation tolerance during the formulation process.