Submitted to: Society of Industrial Microbiology Annual Meeting
Publication Type: Abstract only
Publication Acceptance Date: 7/19/2004
Publication Date: 7/25/2004
Citation: Liu, Z., Slininger, P.J. 2004. Functional genomic studies of in situ detoxification of bioethanol fermentation inhibitors using ethanologenic yeast [abstract]. Society of Industrial Microbiology Annual Meeting. Abstract No. S146. Interpretive Summary:
Technical Abstract: The significance of agriculture as an energy producer has been recognized. Biomass degradation and saccharification using dilute acid hydrolysis generates numerous inhibitory compounds which interfere with subsequent ethanol fermentation. Additional detoxification is needed, which creates complexity, generates additional wastes, and increases the already high cost of bioethanol. Due to the lack of understanding of mechanisms of stress tolerance, few genetically improved tolerant strains are available. At NCAUR, we are studying molecular mechanisms of stress tolerance in order to design and engineer novel strains for effective bioethanol fermentation using integrated functional genomics. We explore global analysis of gene expression in response to fermentation inhibitor stress for ethanologenic yeasts. A better understanding of the genetic mechanisms and biochemical pathways responsible for the stress tolerance in yeasts will provide a sound foundation to design and develop genetically engineered industrial strains to withstand and/or detoxify major inhibitors generated during biomass pretreatment and fermentation.