|Schisler, David - Dave|
|Slininger, Patricia - Pat|
Submitted to: National Fusarium Head Blight Forum Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/15/2003
Publication Date: 12/15/2003
Citation: Schisler, D.A., Slininger, P.J., Behle, R.W., Zhang, S., Boehm, M.J., Lipps, P.E., Palmquist, D.E. 2003. USDA-ARS, Ohio State University cooperative research on biologically controlling fusarium head blight 1: In vitro and field testing of the effect of UV protectants on FHB antagonists. In: Proceedings of the National Fusarium Head Blight Forum, December 13-15, 2003, Minneapolis, MN. p. 105-108.
Technical Abstract: In previous research, we have demonstrated the potential of several antagonists including Bacillus subtilis OH 131.1 and Cryptococcus nodaensis OH 182.9, to significantly reduce the severity of Fusarium head blight in wheat field environments when biomass was produced in laboratory and pilot-scale quantities in liquid culture. Formulation research, in terms of UV protectants, may be crucial for the continued development of these biocontrol strains from bench discovery to biocontrol product. UV light is particularly devastating to the survival of vegetative microbial cells. A water soluble sodium salt of lignin (Westvaco PC1307, experimental product) at 0.2 and 0.3% and the optical brightener Blankophor BBH at 0.5, 1.0, and 2.0% enhanced the survival of dried OH 131.1 in an in vitro microtiter plate assay when plates were exposed to 6 h of artificial sunlight. While antagonists reduced FHB in field studies conducted at Peoria, Illinois, and Wooster, Ohio; lignin at 0.3% did not have a consistent influence on OH 131.1 and OH 182.9 efficacy. Testing of additional UV protectants and formulations resistant to wash-off should clarify whether UV protectants can enhance the field efficacy of FHB antagonists.