Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/21/2001
Publication Date: 10/21/2001
Citation: Detvisitsakun, C., Zhang, W., Muthukrishnan, S., Lookhart, G.L., Liang, G.H. 2001. Incorporating a barley HVA1 gene to wheat for drought resistence. Page 242. Poster No. 430L. American Society of Agronomy Meeting Abstract.
Interpretive Summary: Presentation at the American Society of Agronomy Meeting, Charlotte, NC, October 21-25, 2001.
Technical Abstract: To improve drought tolerance, a gene encoding a late embryogenesis abundant (LEA) protein, HVA1, from barley (Hordeum vulgare L.) was introduced into a hard red winter wheat, Jagger, and a hard white winter wheat, Lakin, using biolistic bombardment. The gene construction, containing HVA1 gene driven by the rice Act1 promoter and the selectable marker gene, bar, under the control of CaMV35S promoter, was delivered to embryogenic calli. One transgenic Jagger wheat plant was obtained. This plant survived in medium containing 5 mg/l ammonium glufosinate during the tissue culture processes and has normal morphology. The plant tested positive for the PCR analysis of bar gene and was resistant to 0.1% (v/v) herbicide LibertyTM. Southern blot analysis showed the integration of bar and HVA1 gene into the genome of this plant. The 27 kDa of HVA1 protein also was detected in this plant as shown by Western blot.