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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Citrus and Other Subtropical Products Research » Research » Research Project #444837

Research Project: Utilizing Nuclear Magnetic Resonance to Determine the Effect of Anaerobic Soil Disinfestation on Soil Properties

Location: Citrus and Other Subtropical Products Research

Project Number: 6034-22000-046-016-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2023
End Date: Oct 1, 2025

To determine the effect of anaerobic soil disinfestation (ASD) on soil chemical properties with an emphasis on anti-microbial metabolites produced during anaerobic soil disinfestation. Preliminary research using nuclear magnetic resonance has shown ASD treated soil contains various types of antimicrobial compounds, some of which were unknown. To identify these compounds and determine if their production is dependent on soil amendment and are geographically or temporally limited.

Field soil will be amended with molasses and pasteurized broiler litter pellets, tarped with plastic, watered to field capacity, and undisturbed for three-weeks; the technique known as anaerobic soil disinfestation (ASD). ASD-treated soil will be compared to non-treated soil. Soil samples will be taken at various locations in Florida, that differ in soil types, throughout the three-week treatment. Soil samples will be sent to Central State University to be analyzed by nuclear magnetic resonance (NMR) spectroscopy. Water soluble biochemical compounds will be extracted and analyzed. Carbon and proton chemical shifts will be recorded using HSQC spectra and compared to the COLMAR and BMRB databases. Metabolite composition of the different samples will be compared using principal component analysis.