Location: Dairy and Functional Foods Research
Project Number: 8072-41000-108-011-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 10, 2021
End Date: Aug 9, 2022
Objective:
The proposed collaborative research project will evaluate if triclosan (TCS)-induced colitis and gut dysbiosis can be stopped and/or recovered when the use of the antibiotics is suspended. It has been shown that the antibiotics has a strong effect on colonic inflammation and tumorigenesis in vivo on a mice model. It has also been demonstrated in vitro that the destruction of the gut microbial community caused by high-dose TCS was recoverable in a few weeks by treating the animal simply with TCS-free regular feed. Here we seek to study the broad effects of TCS on gut microbiota and gastrointestinal health in vivo.
Approach:
TCS will be dissolved in PEG400, then compounded with regular mouse diets at 80 ppm. Mouse strain C57BL/6J will be used in the designed experiment. Five mice will be housed in one cage, 60 mice will be evenly and randomly divided into 4 groups. Group #1 (Control 1) will be fed with regular diet for 4 weeks. Group #2 (Control 2) will be fed with regular diet for 8 weeks. Group #3 will be fed with TCS containing diet for 4 weeks. Group #4 will be fed with TCS containing diet for 4 weeks followed by regular diet for additional 4 weeks. Each group will need 3 cages mice.
On the last day of each week, fecal samples will be collected from each cage, stored anaerobically at 4 Degree Celsius for 16S rRNA and SCFA, acylcarnitines, amino acid, and bile salt analysis. At the end of the experimental period designed for each group, blood samples from each mouse will be collected for the analysis of IL-6 and TCS content in plasma; the weight of the colon and spleen and the length of the colon of tested and control mice will be measured. The colonic tissue will be stained with H&E and evaluated for histology; the samples from the colonic tissue will also be submitted to RNAseq analysis.
All these data, the microbiota, small molecules (SCFA, acylcarnitine, amino acid, and bile acid), and the host response (colonic RNAseq) will be submitted to integrative high dimensional data analysis.
