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Research Project: Metabolic and Epigenetic Regulation of Nutritional Metabolism

Location: Children's Nutrition Research Center

2022 Annual Report

We will: 1)study the effect of enteral nutrition on the downstream signaling pathways and metabolism;2)study if increased FGF19 availability controls rate of growth, tissue protein synthesis and intestinal development;3)study if being born prematurely blunts protein and glucose metabolic responses to the feeding-induced rise in amino acids and insulin; 4)identify by which amino acids, regulate protein synthesis, degradation, and accretion and how responses change with age;5)removed due to investigator departure;6)study molecular mechanisms and functional significance of differences in gene expression identified in satellite cell-derived myoblasts;7)study the impact of maternal dietary protein level during lactation;8)study if vitamin D receptors in the brain are critical for glucose regulation;9)study if leptin is involved in the regulation of gluconeogenesis via the leptin receptor and if leptin agonist and small doses of hypoglycin-A/B reduces gluconeogenesis;10)study the role of the SIRT3 in regulation of pyruvate carboxylase and the gluconeogenesis pathway;11)alter DNA methylation in specific subpopulations of hypothalamic neurons and evaluate lifelong effects on energy metabolism, food intake, and PA;12)find the causes of interindividual epigenetic variation and consequences for human energy balance;13)study the functional impact of folic acid supplementation and in intestinal carcinogenesis;14)study the effect of adiposity, adipokine dysregulation, insulin resistance and vitamin D concentrations on bone and endothelial function; 15)study the effect of vitamin D therapy on change in bone and endothelial function;16)removed due to investigator departure;17)study the CNS circuit architecture and explore circuit complexities that regulate non-homeostatic feeding behaviors via environmental signals transduced by epigenetic mechanisms;18)study the tumorigenic effects of HFCS on a humanized colon tumor mouse model;19)study the effects of HFCS on the gut microbiota of a humanized colon tumor mouse model;20)study the role of HFCS-induced gut microbiota in CRC development; create multi-omic nutritional data share portal to resolve the unmet demand for an efficient access to the large volumes of heterogeneous multi-omic data across various research labs;21)integrate heterogeneous multi-omic datasets such as genetic (SNPs), transcriptomic, epigenetic, proteomic, metabolomic and microbiome to infer molecular network structures illustrating eating disorder dynamics;&22)decode genetic and epigenetic patterns of disordered eating using machine learning methods.

This research will be accomplished using a variety of models and scientific tools to simulate the human newborn and/or child. Researchers will use neonatal piglet and rodent models to fill these knowledge gaps. We will determine whether being born prematurely blunts the anabolic response to feeding and identify mechanisms by which amino acids, particularly leucine, regulate lean growth. Additionally we will use various rodent models to test leptin's effect on gluconeogenesis that is independent of body weight, and will utilize in vitro experiments employing primary hepatocytes. Scientists will also integrate both detailed studies of animal models and characterization of epigenetic mechanisms in humans. We will use mouse models of developmental epigenetics in the hypothalamus to understand cell type-specific epigenetic mechanisms mediating developmental programming of body weight regulation. Mouse models will also be used to investigate how folic acid intake affects epigenetic mechanisms regulating intestinal epithelial stem cell (IESC) development and characterize the involvement of these mechanisms in metabolic programming related to obesity, inflammation, and gastrointestinal cancer. In human studies, we will identify human genomic loci at which interindividual variation in DNA methylation is both sensitive to maternal nutrition in early pregnancy and associated with risk of later weight gain. We will also examine whether restoration of vitamin D sufficiency, in a randomized placebo controlled study design, has a positive effect on bone microarchitecture, bone biomarkers and endothelial function. Studies will be conducted in mice that will uncover the molecular basis of interrelationships among dietary sugar, gut microbiota, and CRC development and identify sugar-induced metabolites and/or microbes that can serve as new biomarkers and targets. Researchers will also conduct a multi-omic integrative study to systematically decipher the molecular interplay of disordered eating and neuron specific brain circuits that control feeding behavior.

Progress Report
To review the progress made during the year, please refer to the following projects: 3092-51000-065-01S (Project #1), 3092-51000-065-02S (Project #2), 3092-51000-065-03S (Project #3) and 3092-51000-065-04S (Project #4).

1. Vitamin D receptors in the brain are important for blood glucose regulation in males but not females. Vitamin D deficiency is associated with diabetes, but the mechanisms are unclear. We had evidence that vitamin D receptors in the paraventricular hypothalamus were important for regulating blood glucose in male mice however, we had not determined if females behaved similarly. Researchers at the Children's Nutrition Research Center in Houston, Texas, used two different mouse models in which there is a loss of the vitamin D receptor in the paraventricular hypothalamus of the brain to determine the effect in female mice. In both of our mouse models, we found that male mice that had decreased vitamin D receptors in that area of the brain had higher glucose levels than their controls, whereas female mice showed no effect of the loss of the vitamin D receptors. These findings of sex differences in mice are important to human studies investigating the role of vitamin D deficiency and overall treatment and prevention of type 2 diabetes based upon the individual's sex.

2. SIRT3 protects the heart against damaging side effects of anti-cancer drug. The chemotherapy drug Doxorubicin is used to treat breast cancer, bladder cancer, ovarian cancer, lymphoma, and leukemia and despite its benefits it may also cause damage to the heart. In searching for ways to alleviate the toxicity of Doxorubicin, researchers at the Children's Nutrition Research Center in Houston, Texas, have found that a metabolic regulating enzyme, SIRT3, offers protection against the heart impacting side-effects of Doxorubicin. We generated two mouse models with elevated heart expression of two SIRT3 isoforms, treated these mice with doxorubicin and investigated the damage to the heart using ultra-sound and other methods. We found that increased SIRT3 expression in the heart protected mice against doxorubicin-induced damage to the structure and function of the heart. SIRT3 could be a potential therapeutic target for mitigating doxorubicin-induced heart-damage. Since we have previously shown that fasting or dietary restriction increased SIRT3 expression in various tissues, fasting or dietary restriction might also mitigate the side effect of doxorubicin.

3. A dietary lipid, palmitoleic acid, suppresses liver glucose production through suppression of SIRT3. The production of glucose by the liver plays a crucial role in maintaining glucose levels during starvation however, uncontrolled glucose production by the liver contributes to elevated blood glucose in individuals with diabetes. Palmitoleic acid is a mono-unsaturated fatty acid that is available from dietary sources and exhibits beneficial effects on diabetes, inflammation, and metabolic diseases. The mechanism by which palmitoleic acid reduces blood glucose is still unclear. Researchers at the Children's Nutrition Research Center in Houston, Texas, conducted a study that revealed that palmitoleic acid reduced liver glucose production and the expression of SIRT3, a key metabolism-regulating enzyme, in high-fat diet fed mice. Overexpression of SIRT3 in the liver or in liver cells enhanced glucose synthesis, and further study revealed that SIRT3 played a role in enhancing the activities of three key enzymes involved in the synthesis of glucose. Our study uncovered the role of SIRT3 in the regulation of liver glucose production and its role in mediating the anti-diabetic effect of palmitoleic acid. These findings may help identify novel treatments of diabetes by palmitoleic acid supplementation or by inhibiting the SIRT3 enzyme.

4. The effect of cardiorespiratory fitness and insulin resistance on bone health in Hispanic children. Obesity appears to have a negative impact on pediatric bone health and insulin resistance may mediate this relationship. It is unclear if cardiorespiratory fitness may have a protective effect on bone health in obese children. Researchers at the Children's Nutrition Research Center in Houston, Texas examined data on a large number of Hispanic youths to evaluate the effect of insulin resistance and cardiorespiratory fitness on bone health. Our results showed that lean body mass is the major determinant of bone mineral content and bone mineral density in Hispanic youth. However, obesity and higher body fat have a negative effect on this relationship, while a higher fitness level was positively related to the measures of bone health. This suggests that greater cardiorespiratory fitness and higher lean mass may reduce the adverse effects of adiposity and insulin resistance on bone health in children. These findings support the importance of promoting an increase in physical activity to prevent the negative impact of obesity on bone health in children.

5. The role of sleep and eating patterns in adiposity gain among preschool-aged children. Short sleep duration (less than 9 hours in preschoolers) is related to risk for obesity in preschool children however, the underlying mechanism(s) are not clear. Researchers at the Children's Nutrition Research Center in Houston, Texas, investigated the relationship between sleep characteristics with body composition and weight-regulating behaviors in preschool-aged children (ages 3 to 5 years old). We examined the relationship between sleep, physical activity and dietary behaviors, and the effect of these behaviors on weight and fat gain a year later and found that children do not consistently meet age-recommended sleep duration. Late sleep timing was related to a delay in morning and evening meals and more caloric intake in the evening, a factor that is related to weight gain in older individuals. Both a decrease in sleep duration and later meal timing were related to fat gain a year later which indicates that insufficient sleep duration influences eating behaviors. This supports the importance of sleep hygiene with appropriate timing and adequate duration in preschool age children.

6. Energy expenditure due to gluconeogenesis in insulin resistance. Insulin resistance is a condition when cells in human muscle, fat, and liver do not respond well to insulin and are unable to easily take up glucose from the circulation of blood. For individuals with insulin resistance, suppression of glucose production from the liver by insulin is impaired, contributing to high blood glucose. Glucose production is energetically costly and may contribute to increased energy expenditure. Researchers at the Children's Nutrition Research Center in Houston, Texas, sought to determine if energy expenditure is attributable to glucose production from the liver in individuals with different types of insulin resistance conditions. We demonstrated that glucose production from the liver is a major contributor to energy expenditure in humans with insulin resistance. The findings from this study are important because they indicate that treatments for diabetes that reduce glucose production from the liver could potentially promote weight gain by decreasing the energy expenditure.

Review Publications
Vonderohe, C., Guthrie, G., Stoll, B., Chacko, S., Dawson, H.D., Burrin, D.G. 2021. Tissue-specific mechanisms of bile acid homeostasis and activation of FXR-FGF19 signaling in preterm and term neonatal pigs. American Journal of Physiology.
Spooner, H., Derrick, S., Maj, M., Manjarin, R., Hernandez, G., Tailor, D., Bastani, P., Fanter, R., Fiorotto, M., Burrin, D.G., LaFrano, M., Sikalidis, A., Blank, J. 2021. High-fructose, high-fat diet alters muscle composition and fuel utilization in a juvenile Iberian pig model of non-alcoholic fatty liver disease. Nutrients. 13(12). Article 4195.
Quaye, E., Chacko, S., Chung, S.T., Brychta, R.J., Chen, K.Y., Brown, R.J. 2021. Energy expenditure due to gluconeogenesis in pathological conditions of insulin resistance. American Journal of Physiology - Endocrinology and Metabolism. 321:E795-E801.
Puri, K., Adachi, I., Bocchini, C., Spinner, J., Denfield, S., Sisley, S., Elias, B., Jimenez-Gomez, A., Price, J., Dreyer, W., Choudhry, S., Tunuguntla, H. 2021. Trends in body mass index and association with outcomes in pediatric patients on continuous flow ventricular assist device support. ASAIO Journal.
Guthrie, G., Vonderohe, C., Burrin, D.G. 2022. Fibroblast growth factor 15/19 expression, regulation, and function: An overview. Molecular and Cellular Endocrinology. 548. Article 111617.
Dewey, K.G., Pannucci, T., Kellie, C.O., Davis, T.A., Donovan, S.M., Kleinman, R.E., Taveras, E.M., Bailey, R.L., Novotny, R., Schneeman, B.O., Stang, J., De Jesus, J., Stoody, E.E. 2021. Development of food pattern recommendations for infants and toddlers 6–24 months of age to support the Dietary Guidelines for Americans, 2020–2025. Journal of Nutrition. 151(10):3113-3124.
Redondo, M.J., Warnock, M.V., Libman, I.M., Bocchino, L.E., Cuthbertson, D., Geyer, S., Pugliese, A., Steck, A.K., Evans-Molina, C., Becker, D., Sosenko, J.M., Bacha, F., and the Type 1 Diabetes TrialNet Study Group. 2021. TCF7L2 genetic variants do not influence insulin sensitivity or secretion indices in autoantibody-positive individuals at risk for type 1 diabetes. Diabetes Care. 44(9):2039-2044.
Todd, J.N., Kleinberger, J.W., Zhang, H., Srinivasan, S., Tollefsen, S.E., Levitsky, L.L., Levitt Katz, L.E., Tryggestad, J.B., Bacha, F., Imperatore, G., Lawrence, J.M., Pihoker, C., Divers, J., Flannick, J., Dabelea, D., Florez, J.C., Pollin, T.I. 2021. Monogenic diabetes in youth with presumed type 2 diabetes: Results from the Progress in Diabetes Genetics in Youth (ProDiGY) collaboration. Diabetes Care. 44(10):2312-2319.
Dewey, K.G., Gungor, D., Donovan, S.M., Madan, E.M., Venkatramanan, S., Davis, T.A., Kleinman, R.E., Taveras, E.M., Bailey, R.L., Novotny, R., Terry, N., Butera, G., Obbagy, J., De Jesus, J., Stoody, E. 2021. Breastfeeding and risk of overweight in childhood and beyond: A systematic review with emphasis on sibling-pair and intervention studies. American Journal of Clinical Nutrition. 114(5):1774-1790.
Astudillo, M., Tosur, M., Castillo, B., Rafaey, A., Siller, A., Nieto, J., Sisley, S., McKay, S., Nella, A., Balasubramanyam, A., Bacha, F., Redondo, M. 2021. Type 2 diabetes in prepubertal children. Pediatric Diabetes. .
Sangild, P., Vonderohe, C., Melendez Hebib, V., Burrin, D.G. 2021. Potential benefits of bovine colostrum in pediatric nutrition and health. Nutrients. 13(8). Article 2551.
Manjarin, R., Boutry-Regard, C., Suryawan, A., Canovas, A., Piccolo, B.D., Maj, M., Abo-Ismail, M., Nguyen, H.V., Fiorotto, M.L., Davis, T.A. 2020. Intermittent leucine pulses during continuous feeding alters novel components involved in skeletal muscle growth of neonatal pigs. Amino Acids. 52:1319–1335.
Bailey, R.L., Stang, J.S., Davis, T., Naimi, T.S., Schneeman, B.O., Dewey, K.G., Donovan, S.M., Novotny, R., Kleinman, R.E., Taveras, E.M., Bazzano, L., Snetselaar, L.G., De Jesus, J., Casavale, K.O., Stoody, E.E., Goldman, J.D., Moshfegh, A.J., Rhodes, D.G., Herrick, K., Koegel, K., Perrine, C., Pannucci, T. 2021. Dietary and complementary feeding practices of U.S. infants, 6-12 months: A narrative review of the Federal nutrition monitoring data. Journal of the Academy of Nutrition and Dietetics.
Michels, K.B., Gunasekara, C., Waterland, R.A. 2022. The role of epigenetics in the developmental origins of health and disease. In: Michels, K.B., editors. Epigenetic Epidemiology. 2nd Edition. Cham, Switzerland: Springer Cham. p. 123-124.
Dewey, K., Bazzano, L., Davis, T., Donovan, S., Taveras, E., Kleinman, R. 2020. The duration, frequency, and volume of exclusive human milk and/or infant formula consumption and nutrient status: A systematic review. Dietary Guidelines Advisory Committee Project.
Dewey, K., Bazzano, L., Davis, T., Donovan, S., Taveras, E., Kleinman, R. 2020. The duration, frequency, and volume of exclusive human milk and/or infant formula consumption and overweight and obesity: A systematic review. Dietary Guidelines Advisory Committee Project.
Dewey, K., Bazzano, L., Davis, T., Donovan, S., Taveras, E., Kleinman, R. 2020. Iron from supplements consumed during infancy and toddlerhood and growth, size, and body composition: A systematic review. Dietary Guidelines Advisory Committee Project.
Dewey, K., Bazzano, L., Davis, T., Donovan, S., Taveras, E., Kleinman, R. 2020. Vitamin D from supplements consumed during infancy and toddlerhood and bone health: A systematic review. 2020 Dietary Guidelines Advisory Committee Project.
Costello, E., Rock, S., Stratakis, N., Eckel, S.P., Walker, D.I., Valvi, D., Cserbik, D., Jenkins, T., Xanthakos, S.A., Kohli, R., Sisley, S., Vasiliou, V., LaMerrill, M.A., Rosen, H., Conti, D.V., McConnell, R., Chatzi, L. 2022. Exposure to per-and polyfluoroalkyl substances and markers of liver injury: A systematic review and meta-analysis. Environmental Health Perspectives. 130(4). Article 46001.
Shi, J., Xu, J., Chen, Y., Li, J., Cui, Y., Shen, L., Li, J., Li, W. 2021. The concurrence of DNA methylation and demethylation is associated with transcription regulation. Nature Communications. 12. Article 5285.
Rudar, M., Naberhuis, J.K., Suryawan, A., Nguyen, H.V., Stoll, B., Style, C.C., Verla, M.A., Olutoye, O.O., Burrin, D.G., Fiorotto, M.L., Davis, T.A. 2021. Intermittent bolus feeding does not enhance protein synthesis, myonuclear accretion, or lean growth more than continuous feeding in a premature piglet model. American Journal of Physiology - Endocrinology and Metabolism. 321(6):E737-E752.
Candler, T., Kessler, N., Gunasekara, C., Ward, K., James, P., Laritsky, E., Baker, M., Dyer, R., Elango, R., Jeffries, D., Waterland, R., Moore, S., Ludgate, M., Prentice, A., Silver, M. 2021. DNA methylation at a nutritionally sensitive region of the PAX8 gene is associated with thyroid volume and function in Gambian children. Science Advances. 7(45). Article eabj1561.
Guo, X., Jiang, X., Chen, K., Liang, Q., Zhang, S., Zheng, J., Ma, X., Jiang, H., Wu, H., Tong, Q. 2022. The role of palmitoleic acid in regulating hepatic gluconeogenesis through SIRT3 in obese mice. Nutrients. 14(7). Article 1482.
Gunasekara, C., Hannon, E., MacKay, H., Coarfa, C., McQuillin, A., St. Clair, D., Mill, J., Waterland, R.A. 2021. A machine learning case-control classifier for schizophrenia based on DNA methylation in blood. Translational Psychiatry. 11(1). Article 412.
Wang, P., Yuan, P., Lin, S., Zhong, H., Zhang, X., Zhuo, Y., Li, J., Che, L., Feng, B., Lin, Y., Xu, S., Wu, D., Burrin, D.G., Fang, Z. 2022. Maternal and fetal bile acid homeostasis regulated by sulfated progesterone metabolites through FXR signaling pathway in a pregnant sow model. International Journal of Molecular Sciences. 23. Article 6496.
Joshi, T.P., Fiorotto, M.L. 2021. Variation in AIN-93G/M diets across different commercial manufacturers: Not all AIN-93 diets are created equal. Journal of Nutrition. 151(11):3271-3275.
Gandhi, A.A., Wilson, T.A., Sisley, S., Elsea, S., Foster, R.H. 2022. Relationships between food-related behaviors, obesity, and medication use in individuals with Smith-Magenis syndrome. Research in Developmental Disabilities. 127. Article 104257.
Beck, J., Da Silva Teixeira, S., Harrison, K., Phillips, G., He, Y., Sisley, S. 2022. Paraventricular vitamin D receptors are required for glucose tolerance in males but not females. Frontiers in Endocrinology. 13. Article 869678.
Goetz, A.R., Jindal, I., Moreno, J.P., Puyau, M.R., Adolph, A.L., Musaad, S., Butte, N.F., Bacha, F. 2022. The role of sleep and eating patterns in adiposity gain among preschool-aged children. American Journal of Clinical Nutrition.
Gebara, N.Y., Kim, J.Y., Bacha, F., Lee, S., Arslanian, S. 2022. Metabolic inflexibility in youth with obesity: Is it a feature of obesity or distinctive of youth who are metabolically unhealthy? Clinical Obesity. 12(2). Article e12501.
The TODAY Study Group, Shah, A.S., El Ghormi, L., Gidding, S.S., Hughan, K.S., Levitt Katz, L.E., Koren, D., Tryggestad, J.B., Bacha, F., Braffett, B.H., Arslanian, S., Urbina, E.M. 2021. Longitudinal changes in vascular stiffness and heart rate variability among young adults with youth-onset type 2 diabetes: Results from the follow-up observational treatment options for type 2 diabetes in adolescents and youth (TODAY) study. Acta Diabetologia. 59(2):197-205.
Shah, A.S., Gidding, S.S., El Ghormli, L., Tryggestad, J.B., Nadeau, K.J., Bacha, F., Levitt Katz, L.E., Willi, S.M., Lima, J., Urbina, E.M., TODAY Study Group. 2022. Relationship between arterial stiffness and subsequent cardiac structure and function in young adults with youth-onset type 2 diabetes: Results from the TODAY study. Journal of the American Society of Echocardiography. 35(6):620-628.
Bacha, F., Cheng, P., Gal, R.L., Beaulieu, L.C., Kollman, C., Adolph, A., Shoemaker, A.H., Wolf, R., Klingensmith, G.J., Tamborlane, W.V. 2021. Racial and ethnic disparities in comorbidities in youth with type 2 Diabetes in the Pediatric Diabetes Consortium (PDC). Diabetes Care. 44:2245-2251.
Manjarin, R., Dillard, K., Coffin, M., Hernandez, G.V., Smith, V.A., Noland-Lidell, T., Gehani, T.R., Smart, H.J., Wheeler, K., Sprayberry, K.A., Edwards, M.S., Fanter, R.K., Glanz, H., Immoos, C., Santiago-Rodriguez, T.M., Blank, J.M., Burrin, D.G., Piccolo, B., Abo-Ismail, M., La Frano, M.R., Maj, M. 2022. Dietary fat composition shapes bile acid metabolism and severity of liver injury in a pig model of pediatric NAFLD. American Journal of Physiology - Endocrinology and Metabolism. 323:E187-E206.
Henriksen, N.L., Hansen, S.H., Lycas, M.D., Pan, X., Eriksen, T., Johansen, L.S., Sprenger, R.R., Ejsing, C.S., Burrin, D.G., Skovgaard, K., Christensen, V.B., Thymann, T., Pankratova, S. 2022. Cholestasis alters brain lipid and bile acid composition and compromises motor function in neonatal piglets. Physiological Reports. 10(13). Article e15368.
Tomczyk, M.M., Cheung, K.G., Xiang, B., Tamanna, N., Fonseca Teixeira, A.L., Argawal, P., Kereliuk, S.M., Spicer, V., Lin, L., Treberg, J., Tong, Q., Dolinsky, V.W. 2022. Mitochondrial sirtuin-3 (SIRT3) prevents doxorubicin-induced dilated cardiomyopathy by modulating protein acetylation and oxidative stress. Circulation: Heart Failure. 15(5). Article e008547.
Li, D., Yang, C., Zhu, J., Lopez, E., Zhang, T., Tong, Q., Peng, C., Lin, L. 2021. Berberine remodels adipose tissue to attenuate metabolic disorders by activating sirtuin 3. Acta Pharmacologica Sinica. 43:1285-1298.
Neupane, R., Youker, K., Yalamanchili, H.K., Cieslik, K.A., Karmouty-Quintana, H., Guha, A., Thandavarayan, R.A. 2022. Cleavage stimulating factor 64 depletion mitigates cardiac fibrosis through alternative polyadenylation. Biochemical and Biophysical Research Communications. 597:109-114.
Zhou, J., Hamdan, H., Yalamanchili, H.K., Pang, K., Pohodich, A.E., Lopez, J., Shao, Y., Oses-Prieto, J.A., Li, L., Kim, W., Durham, M.A., Bajikar, S.S., Palmer, D.J., Ng, P., Thompson, M.L., Bebin, E.M., Muller, A.J., Kuechler, A., Kampmeier, A., Haak, T.B., Burlingame, A.L., Liu, Z., Rasband, M.N., Zoghbi, H.Y. 2022. Disruption of MeCP2-TCF20 complex underlies distinct neurodevelopmental disorders. Proceedings of the National Academy of Sciences (PNAS). 119(4). Article e2119078119.
May, T., De La Haye, B., Nord, G., Klatt, K., Stephenson, K., Adams, S., Bollinger, L., Hanchard, N., Arning, E., Bottiglieri, T., Maleta, K., Manary, M., Jahoor, F. 2022. One-carbon metabolism in children with marasmus and kwashiorkor. EBioMedicine. 75. Article 103791.
Moreno, J.P., Hannay, K.M., Walch, O., Dadabhoy, H., Christian, J., El-Mubasher, A., Bacha, F., Grant, S.R., Park, R.J., Cheng, P. 2022. Estimating circadian phase in elementary school children: Leveraging advances in physiologically-informed models of circadian entrainment and wearable devices. Sleep.
Shawar, R.S., Puyau, M., Shypailo, R., Musaad, S., Butte, N.F., Bacha, F. 2022. Adiposity, insulin resistance, cardiorespiratory fitness and bone health in Hispanic children. Journal of Clinical Endocrinology and Metabolism.
Yalamanchili, H.K., Elrod, N.D., Jensen, M.K., Ji, P., Lin, A., Wagner, E.J., Liu, Z. 2021. A computational pipeline to infer alternative poly-adenylation from 3' sequencing data. In: Tian, B. editor. Methods in Enzymology. 1st edition. Cambridge, MA: Academic Press. p. 185-204.
Jensen, M.K., Elrod, N.D., Yalamanchili, H.K., Ji, P., Lin, A., Liu, Z., Wagner, E.J. 2021. Application and design considerations for 3'-end sequencing using click-chemistry. In: Tian, B. editor. Methods in Enzymology. 1st edition. Cambridge, MA: Academic Press. p. 1-23.
Dharmalingam, P., Mahalingam, R., Yalamanchili, H.K., Weng, T., Karmouty-Quintana, H., Guha, A., Thandavarayan, R.A. 2021. Emerging roles of alternative cleavage and polyadenylation (APA) in human disease. Journal of Cellular Physiology. 237:149-160.
Peery, R.C., Pammi, M., Claud, E., Shen, L. 2021. Epigenome - A mediator for host-microbiome crosstalk. Seminars in Perinatology. 45(6). Article 151455.
Spencer, T.E., Wells, K.D., Lee, K., Telugu, B.P., Hansen, P.J., Bartol, F.F., Blomberg, L., Schook, L.B., Dawson, H.D., Lunney, J.K., Driver, J.P., Davis, T.A., Donovan, S.M., Dilger, R.N., Saif, L.J., Moeser, A., McGill, J.L., Smith, G., Ireland, J.J. 2022. Future of biomedical, agricultural and biological systems research using domesticated animals. Biology of Reproduction. 106(4):629-638.
Astudillo, M., Tosur, M., Castillo, B., Refaey, A., Siller, A., Nieto, J., Sisley, S., McKay, S., Nella, A.A., Balasubramanyam, A., Bacha, F., Redondo, M.J. 2021. Type 2 diabetes in prepubertal children. Pediatric Diabetes. 22(7):946-950.
Isart, F.A., Isart-Infante, F.J., Heidel, E.R., Sisley, S. 2022. Acanthosis nigricans is a strong predictor of low blood calcidiol levels in children and adolescents. Metabolic Syndrome and Related Disorders.
Holgersen, K., Rasmussen, M.B., Carey, G., Burrin, D.G., Thymann, T., Sangild, P. 2022. Clinical outcome and gut development after insulin-like growth factor-1 supplementation to preterm pigs. Frontiers in Pediatrics.