Skip to main content
ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Foodborne Toxin Detection and Prevention Research » People & Locations » Reuven Rasooly

Reuven Rasooly
Foodborne Toxin Detection and Prevention Research
Research Chemist

Phone: (510) 559-6478
Fax: (510) 559-6429

USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY, CA 94710
Projects
Advance the Development of Technologies for Detecting and Determining the Stability and Bioavailability of Toxins that Impact Food Safety and Food Defense
In-House Appropriated (D)
  Accession Number: 430056

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Quantitative bioluminescence assay for measuring Bacillus cereus nonhemolytic enterotoxin complex - (Peer Reviewed Journal)
whISOBAXTM inhibits bacterial pathogenesis and enhances the effect of antibiotics Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Choi, H., Do, P.M., Morroni, G., Brescini, L., Cironi, O., Giacometti, A., Apostolidis, E. 2020. whISOBAXTM inhibits bacterial pathogenesis and enhances the effect of antibiotics. Antibiotics. 9(5):264. https://doi.org/10.3390/antibiotics9050264.
CCD based detector for detection of Abrin toxin activity Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2020. CCD based detector for detection of Abrin toxin activity. Toxins. 12(2). https://doi.org/10.3390/toxins12020120.
In-vitro inhibition of staphylococcal pathogenesis by witch-hazel and green tea extracts Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Molnar, A., Choi, H., Do, P.M., Racicot, K., Apostolidis, E. 2019. In-vitro inhibition of staphylococcal pathogenesis by witch-hazel and green tea extracts. Antibiotics. 8(4):244. https://doi.org/10.3390/antibiotics8040244.
T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2019. T cell receptor Vß9 in method to rapidly detect active staphylococcal enterotoxin type A without live animals. Toxins. 11:399. https://doi.org/10.3390/toxins11070399.
Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2018. Alternative to animal use for detecting biologically active Staphylococcal enterotoxin type A. Toxins. 10(12):540. https://doi.org/10.3390/toxins10120540.
Synthesis of pyrrolidine-based hamamelitannin analogues as quorum sensing inhibitors in Staphylococcus aureus Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Bouton, J., Van Hecke, K., Van Calenbergh, S. 2018. Synthesis of pyrrolidine-based hamamelitannin analogues as quorum sensing inhibitors in Staphylococcus aureus. Beilstein Journal of Organic Chemistry. 14:2822-2828. https://doi.org/10.3762/bjoc.14.260.
Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Magoz, Z., Luo, J., Do, P.M., Hernlem, B.J. 2018. Sensitive low-cost CCD-based detector for determination of UV-LED water microbial disinfection. Desalination and Water Treatment. 118:120-125. https://doi.org/10.5004/dwt.2018.22667.
Low cost bioluminescence imaging as an alternative to in vivo bioassays for quantifying biologically active staphylococcal enterotoxin type E Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Low cost bioluminescence imaging as an alternative to in vivo bioassays for quantifying biologically active staphylococcal enterotoxin type E. Sensors and Actuators B: Chemical. 259:357-393. https://doi.org/10.1016/j.snb.2017.12.079.
Detection of abrin holotoxin using novel monoclonal antibodies Reprint Icon - (Peer Reviewed Journal)
He, X., Patfield, S.A., Cheng, L.W., Stanker, L.H., Rasooly, R., McKeon, T.A., Zhang, Y., Brandon, D.L. 2017. Detection of abrin holotoxin using novel monoclonal antibodies. Toxins. 9(12):386. https://doi.org/10.3390/toxins9120386.
TCR-Vß8 as alternative to animal testing for quantifying active SEE Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., He, X., Hernlem, B.J. 2017. TCR-Vß8 as alternative to animal testing for quantifying active SEE. Journal of Environmental and Analytical Toxicology. 7(6):527. https://doi.org/10.4172/2161-0525.1000527.
Interleukin 2 secretion by T cells for detection of biologically active Staphylococcal enterotoxin type E Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Interleukin 2 secretion by T cells for detection of biologically active Staphylococcal enterotoxin type E. Journal of Food Protection. 80(11):1857-1862. https://doi.org/10.4315/0362-028X.JFP-17-196.
Butterfly extracts show antibacterial activity Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Balaban, N., Wolferstan, P., Nash, R., Yael, G. 2017. Butterfly extracts show antibacterial activity. Journal of Agricultural and Food Chemistry. 7:467-479. doi:org/10.4236/aim.2017.76036.
Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays Reprint Icon - (Peer Reviewed Journal)
He, X., Patfield, S.A., Rasooly, R., Mavrici, D. 2017. Novel monoclonal antibodies against Stx1d and 1e and their use for improvement of immunoassays. Journal of Immunological Methods. 44:52-56.
Low cost charged-coupled device (CCD) based detectors for Shiga toxins activity analysis ()
Rasooly R., Prickril B., Bruck H.A., Rasooly A. 2017. Low-cost charged-coupled device (CCD) based detectors for Shiga toxins activity analysis. In: Rasooly A., Prickril B., editors. Biosensors and Biodetection. Methods in Molecular Biology. Volume 1571. New York, NY: Humana Press. p. 233-249. doi: 10.1007/978-1-4939-6848-0 15
Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2017. Rapid cell-based assay for detection and quantification of active staphylococcal enterotoxin type D. Journal of Food Science. doi: 10.1111/1750-3841.13634.
Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Sensitive, rapid, quantitative and in vitro method for the detection of biologically active staphylococcal enterotoxin type E. Toxins. 8(5):150. doi: 10.3390/toxins8050150.
Improving the sensitivity and functionality of mobile webcam-based fluorescence detectors for point-of-care diagnostics in global health ()
Rasooly, R., Hugh, B., Balsom, J., Prickril, B., Ossandon, M., Rasooley, A. 2016. Improving the sensitivity and functionality of mobile webcam-based fluorescence detectors for point-of-care diagnostics in global health. Diagnostics. 6:(2). doi: 10.3390/diagnostics6020019.
Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1 Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2016. Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1. Journal of Agricultural and Food Chemistry. 80:405-410.
A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef Reprint Icon - (Peer Reviewed Journal)
He, X., Kong, Q., Patfield, S.A., Skinner, C.B., Rasooly, R. 2016. A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef. PLoS One. 11(1):e0148092.
Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2015. Plant compounds enhance assay sensitivity for detection of active bacillus cereus toxin. Toxins. 7(3):835-845. doi: 10.3390/toxins7030835.
Sensitive detection of active Shiga toxin using low cost CCD based optical detector Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Balsam, J., Hernlem, B.J., Rasooly, A.J. 2015. Sensitive detection of active Shiga toxin using low cost CCD based optical detector. Biosensors and Bioelectronics. 68:705-711.
Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2014. Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells. Sensors. 14:8869-8876 DOI:10.3390/s140508869.
Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits Reprint Icon - (Peer Reviewed Journal)
Kong, Q., Wu, A., Qi, W., Qi, R., Carter, J.M., Rasooly, R., He, X. 2014. Electron-beam is effective in reducing Escherichia coli on blueberries and extending the shelf life of the fruits. Postharvest Biology and Technology. 95(2014):28-35.
Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.
A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples - (Peer Reviewed Journal)
He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368.
Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.
Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells Reprint Icon - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., Friedman, M. 2013. Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells. PLoS One. 8(8):e71682. doi:10.1371/journal.pone.0071682.
Thousand-fold fluorescent signal amplification for mHealth diagnostics Reprint Icon - (Peer Reviewed Journal)
Balsam, J., Rasooly, R., Bruck, H.A., Rasooly, A. 2013. Thousand-fold fluorescent signal amplification for mHealth diagnostics. Biosensors and Bioelectronics. 51:1-7.
Review of the inhibition of biological activities of selected food-related toxins by natural compounds - (Peer Reviewed Journal)
Friedman, M., Rasooly, R. 2013. Review of the inhibition of biological activities of selected food-related toxins by natural compounds. Toxins. 5:743-775. doi: 10.3390/toxins50x100x.
Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2 Reprint Icon - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760.
Milk inhibits the biological activity of ricin - (Peer Reviewed Journal)
Rasooly, R., He, X., Friedman, M. 2012. Milk inhibits the biological activity of ricin. Journal of Biological Chemistry. 287(33):27924-27929. doi:10.1074/jbc.M112.362988.
TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2012. TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food. Sensors. 12:5978-5985. doi:10.3390/s120505978.
Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract) - (Abstract Only)
Hernlem, B.J., Rasooly, R. 2012. Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract). Meeting Abstract. Poster no. B266.
Sensitive bioassay for detection of biologically active ricin in food - (Peer Reviewed Journal)
Rasooly, R., He, X. 2012. Sensitive bioassay for detection of biologically active ricin in food. Journal of Food Protection. 75(5):951-954.
CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2011. CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A. FEMS Immunology and Medical Microbiology. 64(2):169-174.
Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Rasooly, R. 2012. Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum. Biocatalysis and Agricultural Biotechnology. 1(2):105-109. doi:10.1016/j.bcab.2011.08.016.
Food compounds inhibit Staphylococcus aureus bacteria and the toxicity of Staphylococcus Enterotoxin A (SEA) associated with atopic dermatitis ()
Rasooly, R., Friedman, M. 2012. Food compounds inhibit Staphylococcus aureus bacteria and the toxicity of Staphylococcus Enterotoxin A (SEA) associated with atopic dermatitis. In: Esparza-Gordillo, J., editor. Atopic Dermatitis-Disease Etiology and Clinical Management. Croatia: Intech Europe. p. 387-404
The olive compound 4-hydroxytyrosol inactivates Staphyloccoccus aureus bacteria and Staphylococcal enterotoxin A (SEA) - (Peer Reviewed Journal)
Friedman, M., Rasooly, R., Do, P.M., Henika, P.R. 2011. The olive compound 4-hydroxytyrosol inactivates Staphyloccoccus aureus bacteria and Staphylococcal enterotoxin A (SEA). Journal of Food Science. 76:M558-M558. doi:10.1111/j.1750-3841.2001.02365.x.
Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2011. Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells. Open Journal of Immunology. 1(1):8-14. doi:10.4236/oji.2011.11002.
Clostridium botulinum neurotoxin type B is heat-stable in milk and not inactivated by pasteurization - (Peer Reviewed Journal)
Rasooly, R., Do, P.M. 2010. Clostridium botulinum neurotoxin type B is heat-stable in milk and not inactivated by pasteurization. Journal of Agricultural and Food Chemistry. 58:12557-12561.
Ingested Shiga Toxin 2 (Stx2) Causes Histopathological Changes in Kidney, Spleen and Thymus Tissues and Mortality in Mice - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Griffey, S.M., Vilches-Moure, J.G., Friedman, M. 2010. Ingested Shiga Toxin 2 (Stx2) Causes Histopathological Changes in Kidney, Spleen and Thymus Tissues and Mortality in Mice. Journal of Agricultural and Food Chemistry. (58):9821-9286
Inhibition of Biological Activity of Staphylococcal Enterotoxin A (SEA) by Apple Juice and Apple Polyphenols - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Friedman, M. 2010. Inhibition of Biological Activity of Staphylococcal Enterotoxin A (SEA) by Apple Juice and Apple Polyphenols. Journal of Agricultural and Food Chemistry. 58:5421-5426.
Inhibition of Shiga Toxin 2 (Stx2) in Apple Juices and its Resistance to Pasteurization - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Levin, C.E., Friedman, M. 2010. Inhibition of Shiga Toxin 2 (Stx2) in Apple Juices and its Resistance to Pasteurization. Applied and Environmental Microbiology. Vol. 75(5):M296-M301.
Shiga toxin Stx2 is heat-stable and not inactivated by pasteurization - (Peer Reviewed Journal)
Rasooly, R., Do, P.M. 2009. Shiga toxin Stx2 is heat-stable and not inactivated by pasteurization. International Journal of Food Microbiology. 136(2010):290-294
Detection of Protein Toxins - (Abstract Only)
Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2009. Detection of Protein Toxins. Meeting Abstract. Poster No. 8.
In vitro cell based assay for activity analysis of staphylococcal enterotoxin A in food - (Peer Reviewed Journal)
Rasooly, R., Do, P.M. 2009. In vitro cell based assay for activity analysis of staphylococcal enterotoxin A in food. FEMS Immunology and Medical Microbiology 56(2009): 172-178.
Detection and bioavailability of two biothreat toxins - (Abstract Only)
Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2008. Detection and bioavailability of two biothreat toxins. Meeting Abstract. Poster No. 2.
Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods - (Proceedings)
Carter, J.M., Cheng, L.W., He, X., Rasooly, R., Brandon, D.L., Stanker, L.H. 2010. Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods. Proceedings from Biodetection Technologies 6th Edition. 5:75-95.
Effect of food matrices on the biological activity of ricin - (Peer Reviewed Journal)
He, X., Lu, S.S., Cheng, L.W., Rasooly, R., Carter, J.M. 2008. Effect of food matrices on the biological activity of ricin. Journal of Food Protection 71(10):2053-2058.
In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food - (Peer Reviewed Journal)
Rasooly, R., Do, P.M. 2008. In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food. Applied and Environmental Microbiology. 74(14):4309-4313
Bactericidal activity of the food color additive Phloxine B against Staphylococcus aureus and other food borne microbial pathogens - (Abstract Only)
Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay - (Peer Reviewed Journal)
Rasooly, R., Stanker, L.H., Carter, J.M., Do, P.M., Cheng, L.W., He, X., Brandon, D.L. 2008. In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food. International Journal of Food Microbiiology. 126 (2008):135-139
Docosahexaenoic Acid (DHA) But Not Eicosapentaenoic Acid (EPA) Reverses Trans-10, Cis-12 Conjugated Linoleic Acid Induced Insulin Resistance in Mice1 - (Peer Reviewed Journal)
Vemuri, M., Kelley, D.S., Mackey, B.E., Rasooly, R., Bartolini, G.L. Docosahexaenoic Acid (DHA) But Not Eicosapentaenoic Acid (EPA) Reverses Trans-10, Cis-12 Conjugated Linoleic Acid Induced Insulin Resistance in Mice. Metabolic Syndrome and Related Disorders, 2007 Vol.5, No.4:315-322.
Phloxine B, aversatile bacterial stain - (Peer Reviewed Journal)
Rasooly, R. 2007. Phloxine B, aversatile bacterial stain. International Journal of Food Microbiology.157: 29-34.
DIETARY T10, C-12-CONJUGATED LINOLEIC ACID REDUCES THE EXPRESSION OF FATTY ACID OXIDATION AND DRUG DETOXIFICATION ENZYMES IN MOUSE LIVER - (Peer Reviewed Journal)
Rasooly, R., Kelley, D.S., Greg, J., Mackey, B.E. Dietary t10, c-12-conjugated linoleic acid reduces the expression of fatty acid oxidation and drug detoxification enzymes in mouse liver. Journal of Nutrition. 97:58-66, 2007.
CONSUMPTION OF BING SWEET CHERRIES LOWERS CIRCULATING CONCENTRATIONS OF INFLAMMATION MARKERS IN HEALTHY MEN AND WOMEN - (Peer Reviewed Journal)
Kelley, D.S., Rasooly, R., Jacob, R.A., Kader, A.A., Mackey, B.E. 2006. CONSUMPTION OF BING SWEET CHERRIES LOWERS CIRCULATING CONCENTRATIONS OF INFLAMMATION MARKERS IN HEALTHY MEN AND WOMEN. Journal of Nutrition. 136:981-986, 2006.
RETINOID X RECEPTOR AGONISTS INCREASE BCL2A1 EXPRESSION AND DECREASE APOPTOSIS OF NAÏVE T LYMPHOCYTES - (Peer Reviewed Journal)
Rasooly, R., Schuster, G.U., Gregg, J.P., Xiao, J.H., Chandraratna, R., Stephensen, C.B. 2005. RETINOID X RECEPTOR AGONISTS INCREASE BCL2A1 EXPRESSION AND DECREASE APOPTOSIS OF NAÏVE T LYMPHOCYTES. Journal of Immunology. 175:7916-7929.
ARS News Articles