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ARS Home » Plains Area » Kerrville, Texas » Knipling-Bushland U.S. Livestock Insects Research Laboratory » LAPRU » People & Locations » Pia Olafson

Pia Untalan Olafson (PhD)
Livestock Arthropod Pests Research
Research Molecular Biologist

Phone: (830) 792-0322
Fax: (830) 792-0314

2700 FREDERCKSBURG ROAD
KERRVILLE, TX 78028
Projects
Integrated Pest Management of Flies of Veterinary Importance
In-House Appropriated (D)
  Accession Number: 436658
Integrated Pest Management of Cattle Fever Ticks
In-House Appropriated (D)
  Accession Number: 436694
Preventing Bovine Babesiosis by Characterizing Fever Tick Acaricide Resistance and Genetic Variation Within Vaccine Candidate Antigens
Reimbursable Cooperative Agreement (R)
  Accession Number: 435195
Whole Genome Sequencing of the Face Fly, Musca Autumnalis
Non-Assistance Cooperative Agreement (S)
  Accession Number: 433588
Year 2 CFT Eradication Program Funding - Are Nilgai Antelope and Axis Deer Susceptible to Infection with a Virulent Strain of Babesia bovis?
Non-Assistance Cooperative Agreement (S)
  Accession Number: 437038

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Association of salivary acetylcholinesterase with arthropod vectors of disease Reprint Icon - (Peer Reviewed Journal)
Temeyer, K.B., Schlechte, K.G., Olafson, P.U., Drolet, B.S., Tidwell, J.P., Osbrink, W.L., Showler, A., Gross, A., Perez De Leon, A.A. 2020. Association of salivary acetylcholinesterase with arthropod vectors of disease. Journal of Medical Entomology. https://doi.org/10.1093/jme/tjaa096.
Tracking the increase of acaricide resistance in an invasive population of cattle fever ticks, Rhipicephalus microplus (Acari: Ixodidae), and implementation of real-time PCR assays to rapidly genotype resistance mutations Reprint Icon - (Peer Reviewed Journal)
Thomas, D.B., Klafke, G., Busch, J.D., Olafson, P.U., Miller, R., Mosqueda, J., Stone, N., Scoles, G.A., Wagner, D., Perez De Leon, A.A. 2020. Tracking the increase of acaricide resistance in an invasive population of cattle fever ticks, Rhipicephalus microplus (Acari: Ixodidae), and implementation of real-time PCR assays to rapidly genotype resistance mutations. Annals of the Entomological Society of America. 113(4):298-309. https://doi.org/10.1093/aesa/saz053.
High rate of sex chromosome turnover in muscid flies Reprint Icon - (Peer Reviewed Journal)
Meisel, R.P., Olafson, P.U., Guerrero, F., Konganti, K., Benoit, J.B. 2020. High rate of sex chromosome turnover in muscid flies. G3, Genes/Genomes/Genetics. 10(4):1341-1352. https://doi.org/10.1534/g3.119.400923.
Frequency of kdr and kdr-his alleles in stable fly (Diptera: Muscidae) populations from the United States, Costa Rica, France, and Thailand Reprint Icon - (Peer Reviewed Journal)
Olafson, P.U., Kaufman, P.E., Duvallet, G., Solorzano, J., Taylor, D.B., Trout Fryxell, R. 2019. Frequency of kdr and kdr-his alleles in stable fly (Diptera: Muscidae) populations from the United States, Costa Rica, France, and Thailand. Journal of Medical Entomology. Vol 56(4):1145-1149. https://doi.org/10.1093/jme/tjz012.
Cow-calf producers' willingness to pay for bulls resistant to horn flies, Haematobia irritans (L.) (Diptera: Muscidae) Reprint Icon - (Peer Reviewed Journal)
Mckay, L., Delong, K.L., Schexnayder, S., Griffith, A.P., Taylor, D.B., Olafson, P.U., Trout Fryxell, R. 2019. Cow-calf producers' willingness to pay for bulls resistant to horn flies, Haematobia irritans (L.) (Diptera: Muscidae). Journal of Economic Entomology. Vol 112(3):1476-1484. https://doi.org/10.1093/jee/toz013.
Molecular Tools Used in Medical and Veterinary Entomology Reprint Icon ()
Nayduch, D., Olafson, P.U., Trout-Fryxell, R. 2019. Molecular Tools Used in Medical and Veterinary Entomology. Medical and Veterinary Entomology. https://doi.org/10.1016/B978-0-12-814043-7.00028-5.
Tick vector and disease pathogen surveillance of nilgai antelope, Boselaphus tragocamelus, in Southeastern Texas - (Peer Reviewed Journal)
Fungi isolated from house flies (Diptera: Muscidae) on penned cattle in South Texas - (Peer Reviewed Journal)
Persistence of two Salmonella enterica ser. Montevideo strains throughout horn fly (Diptera: Muscidae) larval and pupal development - (Peer Reviewed Journal)
Olafson, P.U., Temeyer, K.B., Lohmeyer, K.H., Edrington, T.S., Loneragan, G. 2017. Persistence of two Salmonella enterica ser. Montevideo strains throughout horn fly (Diptera: Muscidae) larval and pupal development. Annals of the Entomological Society of America. 110(4):54-60.
Neuronal projections from the Haller's organ and palp sensilla to the synganglion of Amblyomma americanum - (Peer Reviewed Journal)
Borges, L.M., Li, A.Y., Olafson, P.U., Renthal, R., Bauchan, G.R., Lohmeyer, K.H., Perez De Leon, A.A. 2016. Neuronal projections from the Haller's organ and palp sensilla to the synganglion of Amblyomma americanum. Revista Brasileira de Parasitologia Veterinaria. 25(2):217-224.
Assessing transmission of Salmonella to bovine peripheral lymph nodes upon horn fly feeding - (Peer Reviewed Journal)
Status of Nilgai antelope as a host of cattle fever tick (Boophilus microplus) and Babesiosis - (Abstract Only)
A virulent babesia bovis strain failed to infect white-tailed deer (Odocoileus virginianus) Reprint Icon - (Peer Reviewed Journal)
Ueti, M.W., Olafson, P.U., Freeman, J.M., Johnson, W.C., Scoles, G.A. 2015. A virulent babesia bovis strain failed to infect white-tailed deer (Odocoileus virginianus). PLoS One. doi: 10.1371/journal.pone.0131018.
Ticks and the Mammalian Meat Allergy Reprint Icon ()
Olafson, P.U. 2015. Ticks and the Mammalian Meat Allergy. Internet Web Page. http://www.beefresearch.org/cmdocs/beefresearch/safety_fact_sheets/ticks%20and%20the%20mammalian%20meat%20allergy%202.pdf.
Multiple mutations in the para-sodium channel gene are associated with pyrethroid resistance in Rhipicephalus microplus from the United States and Mexico - (Peer Reviewed Journal)
Stone, N.E., Olafson, P.U., Davey, R.B., Buckmeier, B.G., Bodine, D.L., Sidak-Loftis, L.C., Duhaime, R.A., Miller, R., Mosqueda, J., Scoles, G.A., Wagner, D.M., Busch, J. 2014. Multiple mutations in the para-sodium channel gene are associated with pyrethroid resistance in Rhipicephalus microplus from the United States and Mexico. Parasites & Vectors. 7:456.
Survival and fate of Salmonella enterica serovar Montevideo in adult Horn Flies (Diptera: Muscidae) - (Peer Reviewed Journal)
Olafson, P.U., Lohmeyer, K.H., Edrington, T.S., Loneragan, G.H. 2014. Survival and fate of Salmonella enterica serovar Montevideo in adult horn flies (Diptera: Muscidae). Journal of Medical Entomology. 51(5):993-1001.
Expression of bovine genes associated with a local and systemic immune response to infestation by the Lone Star tick, Amblyomma americanum - (Peer Reviewed Journal)
Brannon, J.L., Riggs, P.K., Olafson, P.U., Ivanov, I., Holman, P.J. 2014. Expression of bovine genes associated with a local and systemic immune response to infestation by the Lone Star tick, Amblyomma americanum. Ticks and Tick Borne Diseases. 5:676-688.
Widespread Movement of Invasive Cattle Fever Ticks (Rhipicephalus microplus) in Southern Texas Leads to Shared Local Infestations on Cattle and Deer - (Peer Reviewed Journal)
Busch, J.D., Stone, N.E., Nottingham, R., Araya-Anchetta, A., Lewis, J., Hochhalter, C., Giles, J.R., Freeman, J.M., Buckmeier, B.G., Bodine, D.L., Duhaime, R.A., Miller, R., Davey, R.B., Olafson, P.U., Scoles, G.A., Wagner, D.M. 2014. Widespread movement of invasive cattle fever ticks (Rhipicephalus microplus) in southern Texas leads to shared local infestations on cattle and deer. Parasites & Vectors. 7:188.
Invasive Potential of Cattle Fever Ticks in the Southern United States - (Peer Reviewed Journal)
Giles, J.R., Peterson, A.T., Busch, J.D., Olafson, P.U., Scoles, G.A., Davey, R.B., Pound, J.M., Kammlah, D.M., Lohmeyer, K.H., Wagner, D.M. 2014. Invasive potential of cattle fever ticks in the southern United States. Parasites & Vectors. 7:189.
Are biting fly larvae biological reservoirs of Salmonella? - (Abstract Only)
Spatiotemporal Incidence of Acaracide Resistance among Outbreaks of Cattle Fever Ticks in the US - (Abstract Only)
Discovery of microRNAs of the stable fly (Diptera: Muscidae) by high-throughput sequencing - (Peer Reviewed Journal)
Tuckow, A.P., Temeyer, K.B., Olafson, P.U., Perez De Leon, A.A. 2013. Discovery of microRNAs of the stable fly (Diptera: Muscidae) by high-throughput sequencing. Journal of Medical Entomology. 50(4):925-930.
Acetylcholinesterase of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression, and biochemical properties of recombinant proteins - (Peer Reviewed Journal)
Temeyer, K.B., Olafson, P.U., Brake, D.K., Tuckow, A.P., Li, A.Y., Perez De Leon, A.A. 2013. Acetylcholinesterase of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression, and biochemical properties of recombinant proteins. Pesticide Biochemistry and Physiology. 106:118-123.
Molecular Characterization and Immunolocalization of the Olfactory Co-recepter Orco from Two Blood-feeding Muscid Flies, the Stable Fly (Stomoxys calcitrans, L.) and the Horn Fly (Haematobia irritans irritans, L.) - (Peer Reviewed Journal)
Olafson, P.U. 2013. Molecular characterization and immunolocalization of the olfactory co-recepter orco from two blood-feeding muscid flies, the stable fly (Stomoxys calcitrans, L.) and the horn fly (Haematobia irritans irritans, L.). Insect Molecular Biology. 22(2):131-142.
USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory Tick & Biting Fly Research Unit scientific update ()
Evaluations of methods for the isolation of high quality RNA from bovine and cervine hide biopsies for use in gene expression studies - (Peer Reviewed Journal)
Ticks on Deer and Cattle in the Cattle Fever Tick Permanent Quarantine Zone, 2012 - (Abstract Only)
Discovery and functional analysis of small RNAs (miRNA/siRNA) of livestock ectoparasites. - (Abstract Only)
Excluding feral swine, javelina and raccoons from deer bait stations - (Abstract Only)
Acetylcholinesterases of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression and biochemical properties of recombinant proteins - (Abstract Only)
Sequence polymorphism in acetylcholinesterase transcripts and genotyping survey of BmAChE1 in laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus - (Peer Reviewed Journal)
Temeyer, K.B., Olafson, P.U., Pruett, J.H. 2012. Sequence polymorphism in acetylcholinesterase transcripts and genotyping survey of BmAChE1 in laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus. Journal of Medical Entomology. 49(3):555-562.
Analysis of doramectin in the serum of repeatedly treated pastured cattle used to predict the probability of cattle fever ticks (Acari: Ixodidae) feeding to repletion Reprint Icon - (Peer Reviewed Journal)
Davey, R.B., Pound, J.M., Klavons, J.A., Lohmeyer, K.H., Freeman, J.M., Olafson, P.U. 2012. Analysis of doramectin in the serum of repeatedly treated pastured cattle used to predict the probability of cattle fever ticks (Acari: Ixodidae) feeding to repletion. Experimental and Applied Acarology. 56(4):365-374.
Excluding feral swine, javelina, and raccoons from deer bait stations Reprint Icon - (Peer Reviewed Journal)
Pound, J.M., Lohmeyer, K.H., Davey, R.B., Soliz, L.A., Olafson, P.U. 2012. Excluding feral swine, javelina, and raccoons from deer bait stations. Human-Wildlife Interactions. 36:383-385.
Multiple acetylcholinesterase genes expressed in the southern cattle tick, Rhipicephalus (Boophilus) microplus, a vector of bovine babesiosis and anaplasmosis - (Abstract Only)
Multiple acetylcholinesterase genes expressed in the southern cattle tick, Rhipicephalus (Boophilus) microplus, a vector of bovine anaplasmosis and babesiosis - (Abstract Only)
Genetic factors potentially reducing fitness cost of organophosphate-insensitive acetylcholinesterase(s) in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) - (Peer Reviewed Journal)
Temeyer, K.B., Tijerina, M.A., Davey, R.B., Olafson, P.U. 2011. Genetic factors potentially reducing fitness cost of organophosphate-insensitive acetylcholinesterase(s) in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Acarology International Congress Proceedings. Zoosymposia 6:260–266.
Immunological response of tick-naive white-tailed deer to infestation with Dermacentor albipictus, a one host tick - (Abstract Only)
Impact of white-tailed deer and exotic ungulate populations on cattle fever tick outbreaks in South Texas - (Abstract Only)
Overview of the U.S. Cattle Fever Tick Eradication Program: History and Challenges - (Abstract Only)
Novel techniques developed to control cattle fever ticks feeding on free-ranging white-tailed deer along the Rio Grande in South Texas - (Abstract Only)
Preliminary findings of a molecular survey for the presence of B. bovis and B. bigemina in cattle fever ticks and white-tailed deer from south Texas - (Abstract Only)
Are white-tailed deer compromising efforts to maintain eradication of cattle fever ticks from cattle herds in the U.S.? - (Abstract Only)
Identification of a mutation associated with permethrin resistance in the para-type sodium channel of the stable fly (Diptera: Muscidae) - (Peer Reviewed Journal)
Olafson, P.U., Pitzer, J.B., Kaufman, P.E. 2011. Identification of a mutation associated with permethrin resistance in the para-type sodium channel of the stable fly (Diptera: Muscidae). Journal of Economic Entomology. 104(1):250-257.
Bm86 midgut protein sequence variation in south Texas cattle fever ticks - (Peer Reviewed Journal)
Freeman, J.M., Davey, R.B., Kappmeyer, L.S., Kammlah, D.M., Olafson, P.U. 2010. Bm86 midgut protein sequence variation in south Texas cattle fever ticks. Parasites & Vectors. 3;3:101.
Novel approaches for control of Rhipicephalus (Boophilus) microplus - (Peer Reviewed Journal)
Temeyer, K.B., Chen, A.C., Davey, R.B., Guerrero, F., Freeman, J.M., Kammlah, D.M., Li, A.Y., Lohmeyer, K.H., Olafson, P.U., Perez De Leon, A.A., Phillips, P.L., Pound, J.M., Welch, J.B. 2012. Novel approaches for control of Rhipicephalus (Boophilus) microplus. J Tecnica Pecuaria En Mexico. 3(Suppl. 1):25-40.
Multiple transcripts encode glucose 6-phosphate dehydrogenase in the southern cattle tick, Rhipicephalus (Boophilus) microplus Reprint Icon - (Peer Reviewed Journal)
Olafson, P.U., Pruett Jr, J.H., Temeyer, K.B. 2011. Multiple transcripts encode glucose 6-phosphate dehydrogenase in the southern cattle tick, Rhipicephalus (Boophilus) microplus. Experimental and Applied Acarology. 53(2):147-165.
Four transcripts encode glucose 6-phosphate dehydrogenase (G6PDH) in the Southern cattle tick, Rhipicephalus (Boophilus) microplus - (Peer Reviewed Journal)
Insight into stable fly larvae: Salivary gland-specific polypeptides and evidence for genes that may have a role in the stable fly innate immune system - (Proceedings)
Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex-determination - (Peer Reviewed Journal)
Olafson, P.U., Dowd, S.E., Lohmeyer, K.H. 2010. Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex-determination. Archives of Insect Biochemistry and Physiology. 74(3):179-2004.
Baculovirus expression, biochemical characterization and organophosphate sensitivity of rBmAChE1, rBmAChE2, and rBmAChE3 of Rhipicephalus (Boophilus) microplus - (Peer Reviewed Journal)
Temeyer, K.B., Pruett Jr, J.H., Olafson, P.U. 2010. Baculovirus expression, biochemical characterization and organophosphate sensitivity of rBmAChE1, rBmAChE2, and rBmAChE3 of Rhipicephalus (Boophilus) microplus. Veterinary Parasitology. 172:114-121.
Sequence variation of Bm86 in cattle fever ticks isolated from outbreaks in south Texas - (Proceedings)
Genotyping mutation in BmAChE3: A survey of laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus that are resistant or susceptible to coumaphos - (Peer Reviewed Journal)
Temeyer, K.B., Olafson, P.U., Miller, R. 2009. Genotyping mutation in BmAChE3: A survey of laboratory and Mexican strains of Rhipicephalus (Boophilus) microplus that are resistant or susceptible to coumaphos. Journal of Medical Entomology. 46(6):1355-1360.
Serologically defined Rhipicephalus (Boophilus) microplus larval antigens in BmLF3, a partially pure Sephacryl S-300 fraction of crude larval proteins - (Peer Reviewed Journal)
Pruett Jr, J.H., Olafson, P.U., Davey, R.B. 2008. Serologically defined Rhipicephalus (Boophilus) microplus larval antigens in BmLF3, a partially pure Sephacryl S-300 fraction of crude larval proteins. Veterinary Parasitology. 155(3-4):264-272.
ACETYLCHOLINESTERASE MUTATION IN DIAZINON-RESISTANT HAEMATOBIA IRRITANS (L.) (DIPTERA: MUSCIDAE) - (Peer Reviewed Journal)
Temeyer, K.B., Li, A.Y., Lohmeyer, K.H., Chen, A.C., Olafson, P.U., Sanson, D.W., Foil, L.D. 2008. Acetylcholinesterase mutation in diazinon-resistant Haematobia irritans (L.) (Diptera: Muscidae). Veterinary Parasitology. 154(3-4):300-310.
R86Q, a mutation in BmAChE3 yielding a Rhipicephalus microplus organophosphate-insensitive acetylcholinesterase - (Peer Reviewed Journal)
Temeyer, K.B., Pruett Jr, J.H., Olafson, P.U., Chen, A.C. 2007. R86Q, a mutation in BmAChE3 yielding a Rhipicephalus microplus organophosphate-insensitive acetylcholinesterase. Journal of Medical Entomology. 44(6):1013-1018.
Association of the bovine leukocyte antigen major histocompatibility complex class II DRB3*4401 allele with host resistance to the Lone Star Tick, Amblyomma americanum - (Peer Reviewed Journal)
Olafson, P.U., Pruett Jr, J.H., Steelman, C.D. 2007. Association of the bovine leukocyte antigen major histocompatibility complex class II DRB3*4401 allele with host resistance to the Lone Star Tick, Amblyomma americanum. Veterinary Parasitology. 145(1-2):190-195.
Thrombostasin isoform frequency in a Central Texas field population of the horn fly, Haematobia irritans - (Peer Reviewed Journal)
Olafson, P.U., Pruett Jr, J.H., Atteberry, H.N., Steelman, C.D. 2006. Thrombostasin isoform frequency in a Central Texas field population of the horn fly, Haematobia irritans. Veterinary Parasitology. 142:359-366.
IDENTIFICATION AND PARTIAL PURIFICATION OF SEROLOGICALLY DEFINED BOOPHILUS MICROPLUS LARVAL ANTIGENS BY NATURAL ECTOPARASITE EXPOSURE - (Peer Reviewed Journal)
Pruett Jr., J.H., Untalan, P.M., Davey, R.B. 2006. Identification and partial purification of serologically defined Boophilus microplus larval antigens by natural ectoparasite exposure. Veterinary Parasitology. 140:148-157.
BACULOVIRUS EXPRESSION OF BMACHE3, A CDNA ENCODING AN ACETYLCHOLINESTERASE OF BOOPHILUS MICROPLUS (ACARI:IXODIDAE) - (Peer Reviewed Journal)
Temeyer, K.B., Pruett Jr., J.H., Untalan, P.M., Chen, A.C. 2006. Baculovirus expression of BmAChE3, a cDNA encoding an acetylcholinesterase of Boophilus microplus (Acari: Ixodidae). Journal of Medical Entomology. 43(4):707-712.
THE RESISTANCE OF VARROA MITES (ACARI: VARROIDAE) TO ACARICIDES AND THE PRESENCE OF ESTERASE - (Peer Reviewed Journal)
Sammataro, D., Untalan, P., Guerro, F., Finley, J. The resistance of Varroa mites (Acari: Varroidae) to acaricides and the presence of esterase. 2005. Internat. J. Acarol. Vol. 31, No. 1. 67-74.
PROTEOME ANALYSIS OF ABUNDANTLY EXPRESSED PROTEINS FROM UNFED LARVAE OF THE CATTLE TICK, BOOPHILUS MICROPLUS - (Peer Reviewed Journal)
Untalan, P.M., Guerrero, F.D., Haines, L.R., Pearson, T.W. 2005. Proteome analysis of abundantly expressed proteins from unfed larvae of the cattle tick, Boophilus microplus. Insect Biochemistry and Molecular Biology. 35:141-151.
ORGANOPHOSPHATE RESISTANCE IN MEXICAN STRAINS OF BOOPHILUS MICROPLUS: A MAJOR THREAT TO THE U.S. CATTLE INDUSTRY - (Peer Reviewed Journal)
Temeyer, K.B., Pound, J.M., Miller, J.A., Chen, A.C., Pruett Jr, J.H., Guerrero, F., Davey, R.B., Untalan, P.M., Lohmeyer, K.H., Li, A.Y., Miller, R., George, J.E. 2004. Organophosphate resistance in Mexican strains of Boophilus microplus: A major threat to the U.S. cattle industry. Southern Association of Agricultural Scientists Bulletin of Biochemistry and Biotechnology. 17:43-51.
MEMBRANE PROTEINS INDUCED BY ORGANOPHOSPHATE EXPOSURE IN OP-SUSCEPTIBLE AND -RESISTANT STRAINS OF THE SOUTHERN CATTLE TICK - (Proceedings)
GUERRERO, F., LI, A.Y., UNTALAN, P.M. 2003 APRIL 8-11. MEMBRANE PROTEINS INDUCED BY ORGANOPHOSPHATE EXPOSURE IN OP-SUSCEPTIBLE AND -RESISTANT STRAINS OF THE SOUTHERN CATTLE TICK. PRESENTED AT THE 78TH ANNUAL MEETING OF THE SOUTHWESTERN AND ROCKY MOUNTAIN (SWARM) DIVISION OF THE AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE (AAAS). TULSA, OKLAHOMA.