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» Siqing Liu
Siqing Liu
Research Molecular Biologist
Research Molecular Biologist
Renewable Product Technology
1815 N University St.
Peoria IL 61604
+1 (309) 681-6566
Ph.D. Molecular Biology University of Illinois
M.S. Biotechnology Institute of Botany, Chinese Academy of Sciences
B.S. Biology Lanzhou University
Siqing Liu is a Research Molecular Biologist with the USDA Agricultural Research Service. Dr. Liu received her Ph.D. in Plant Molecular Biology from the University of Illinois, Urbana-Champaign. She joined the USDA-ARS-NCAUR in 2002, studying cost-effective and renewable production of fuels and chemicals. Dr. Liu studies a range of microbes including E. coli, Lactobacillus species and Clostridia species in the area of microbiology, molecular biology, microbial genome, proteomics and biotechnology. Dr. Liu has been working on fermentative production of biofuels ethanol/butanol, other value-added bioproducts such as lactic acid, butyric acid as well as specific enzymes and biodegradable antibacterial proteins from lignocellulosic biomass feedstocks.
Dr. Siqing Liu's Google Scholar page
Editor-in-Chief Probiotics and Antimicrobial Proteins Impact factor 5.265 (2021)
Available ARS technologies seeking for collaborations
Lactobacillus paracasei NRRL B-50314
Antimicrobials for Biorefining and Agricultural Applications In-House Appropriated (D) Accession Number:439228 
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- Novel Endolysin LysMP for Control of Limosilactobacillus and Lactobacillus Bacterial Contamination in Small-Scale Corn Mash Fermentation -(Peer Reviewed Journal)
Patel, M., Lu, S.Y., Liu, S., Skory, C.D. 2023. Novel Endolysin LysMP for Control of Limosilactobacillus and Lactobacillus Bacterial Contamination in Small-Scale Corn Mash Fermentation. Biotechnology for Biofuels and Bioproducts. 16 Article 144. https://doi.org/10.1186/s13068-023-02400-5. - Can xylose be fermented to biofuel butanol in continuous long-term reactors: If not, what options are there? -(Peer Reviewed Journal)
Qureshi, N., Lin, X., Tao, S., Liu, S., Huang, H., Nichols, N.N. 2023. Can xylose be fermented to biofuel butanol in continuous long-term reactors: If not, what options are there? Energies. 16(13). Article 4945. https://doi.org/10.3390/en16134945. - Saccharomyces cerevisiae surface display of endolysin LysKB317 for control of bacterial contamination in corn ethanol fermentations -(Peer Reviewed Journal)
Lu, S.Y., Liu, S., Patel, M., Glenzinski, K.M., Skory, C.D. 2023. Saccharomyces cerevisiae surface display of endolysin LysKB317 for control of bacterial contamination in corn ethanol fermentations. Frontiers in Bioengineering and Biotechnology. 11. Article 1162720. https://doi.org/10.3389/fbioe.2023.1162720. - Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain Limosilactobacillus reuteri -(Peer Reviewed Journal)
Selvamani, S., Ramli, S., Dailin, D.J., Natasya, K.H., Varzakas, T., Abomoelak, B., Sukmawati, D., Nurjayadi, M., Liu, S., Gupta, V.K., El Enshasy, H.A. 2022. Extractive fermentation as a novel strategy for high cell mass production of hetero-fermentative probiotic strain Limosilactobacillus reuteri. Fermentation. 8(10). Article 527. https://doi.org/10.3390/fermentation8100527. - Butyric acid production by fermentation: Employing potential of the novel Clostridium tyrobutyricum strain NRRL 67062 -(Peer Reviewed Journal)
Qureshi, N., Liu, S., Saha, B.C. 2022. Butyric acid production by fermentation: Employing potential of the novel Clostridium tyrobutyricum strain NRRL 67062. Fermentation. 8(10). Article 491. https://doi.org/10.3390/fermentation8100491. - Antibacterial property and metagenomic analysis of milk kefir -(Peer Reviewed Journal)
Liu, S., Lu, S.Y., Qureshi, N., El Enshasy, H.A., Skory, C.D. 2022. Antibacterial property and metagenomic analysis of milk kefir. Probiotics and Antimicrobial Proteins. 14:1170-1183. https://doi.org/10.1007/s12602-022-09976-8. - Milk kefir microbial compositions and two isolated cultures with antibacterial activities-(Abstract Only)
Liu, S., Lu, S.Y., Qureshi, N., El Enshasy, H.A., Skory, C.D. 2022. Milk kefir microbial compositions and two isolated cultures with antibacterial activities. [abstract]. Submitted to Society of Industrial Microbiology 72nd Annual Meeting 2022. San Francisco, CA, United States August 7-10, 2022. (https://www.simbhq.org/annual/). - Cellulosic butanol biorefinery: production of biobutanol from high solid loadings of sweet sorghum bagasse - simultaneous saccharification, fermentation, and product recovery -(Peer Reviewed Journal)
Qureshi, N., Saha, B.C., Liu, S., Ezeji, T.C., Nichols, N.N. 2021. Cellulosic butanol biorefinery: production of biobutanol from high solid loadings of sweet sorghum bagasse - simultaneous saccharification, fermentation, and product recovery. Fermentation. 7(4): Article 310. https://doi.org/10.3390/fermentation7040310. - Fermentative production of alternative antimicrobial peptides and enzymes -(Review Article)
Lu, S.Y., Skory, C.D., El Enshasy, H.A., Liu, S. 2021. Fermentative production of alternative antimicrobial peptides and enzymes. Biocatalysis and Agricultural Biotechnology. 37. Article 102189. https://doi.org/10.1016/j.bcab.2021.102189. - Proteomic analysis identifies dysregulated proteins in butanol-tolerant gram-positive Lactobacillus mucosae BR0713-33 -(Peer Reviewed Journal)
Liu, S., Qureshi, N., Bischoff, K., Darie, C.C. 2021. Proteomic analysis identifies dysregulated proteins in butanol-tolerant gram-positive Lactobacillus mucosae BR0713-33. ACS Omega. 6(5):4034-4043. https://doi.org/10.1021/acsomega.0c06028. - Ethanol tolerance assessment in recombinant E. coli of ethanol responsive genes from Lactobacillus buchneri NRRL B-30929 -(Peer Reviewed Journal)
Liu, S., Skory, C.D., Qureshi, N. 2020. Ethanol tolerance assessment in recombinant E. coli of ethanol responsive genes from Lactobacillus buchneri NRRL B-30929. World Journal of Microbiology and Biotechnology. 36. Article 179. https://doi.org/10.1007/s11274-020-02953-9. - Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations -(Peer Reviewed Journal)
Lu, S.Y., Bischoff, K.M., Rich, J.O., Liu, S., Skory, C.D. 2020. Recombinant bacteriophage LysKB317 endolysin mitigates Lactobacillus infection of corn mash fermentations. Biotechnology for Biofuels. 13. Article 157. https://doi.org/10.1186/s13068-020-01795-9. - Global view of biofuel butanol and economics of its production by fermentation from sweet sorghum bagasse, food waste, and yellow top presscake: Application of novel technologies -(Peer Reviewed Journal)
Qureshi, N., Lin, X., Liu, S., Saha, B.C., Mariano, A.P., Polaina, J., Ezeji, T.C., Friedl, A., Maddox, I.S., Klasson, K.T., Dien, B.S., Singh, V. 2020. Global view of biofuel butanol and economics of its production by fermentation from sweet sorghum bagasse, food waste, and yellow top presscake: Application of novel technologies. Fermentation. 6(2). Article 58. https://doi.org/10.3390/fermentation6020058. - Microbial contamination of commercial corn-based fuel ethanol fermentations -(Peer Reviewed Journal)
Rich, J.O., Anderson, A.M., Leathers, T.D, Bischoff, K.M., Liu, S., and Skory, C.D. 2020. Microbial contamination of commercial corn-based fuel ethanol fermentations. Bioresource Technology. 11:100433. https://doi.org/10.1016/j.biteb.2020.100433. - Production of acetone-butanol-ethanol (ABE) from concentrated yellow top presscake using Clostridium beijerinckii P260 -(Peer Reviewed Journal)
Qureshi, N., Saha, B.C., Liu, S., Harry O Kuru, R.E. 2019. Production of acetone-butanol-ethanol (ABE) from concentrated yellow top presscake using Clostridium beijerinckii P260. Journal of Chemical Technology & Biotechnology. 95(3):614-620. https://doi.org/10.1002/jctb.6242. - Increased ethanol tolerance associated with the pntAB locus of Oenococcus oeni and Lactobacillus buchneri -(Peer Reviewed Journal)
Liu, S., Skory, C., Liang, X., Mills, D., Qureshi, N. 2019. Increased ethanol tolerance associated with the pntAB locus of Oenococcus oeni and Lactobacillus buchneri. Journal of Industrial Microbiology and Biotechnology. 46:1547-1556. https://doi.org/10.1007/s10295-019-02209-y. - Bioprocessing of rice husk into monosaccharides and the fermentative production of bioethanol and lactate -(Peer Reviewed Journal)
Montipó, S., Ballesteros, I., Fontana, R.C., Liu, S., Ballesteros, M., Martins, A.F., Camassola, M. 2019. Bioprocessing of rice husk into monosaccharides and the fermentative production of bioethanol and lactate. Cellulose. 26:7309–7322. https://doi.org/10.1007/s10570-019-02571-1. - Yellow top (Physaria fendleri) presscake: a novel substrate for butanol production and reduction in environmental pollution -(Peer Reviewed Journal)
Qureshi, N., Harry-O'Kuru, R.E., Liu, S., Saha, B. 2018. Yellow top (Physaria fendleri) presscake: a novel substrate for butanol production and reduction in environmental pollution. Biotechnology Progress. 35(3):e2767. https://doi.org/10.1002/btpr.2767. - Microbiota-derived lactate accelerates intestinal stem cell-mediated epithelial development through the Gpr81 -(Peer Reviewed Journal)
Lee, Y., Kim, T., Kim, Y., Lee, S., Kim, S., Kang, S., Yang, J., Baek, I., Sung, Y., Park, Y., Hwang, S., O, E., Kim, K., Liu, S., Kamada, N., Gao, N., Kweon, M. 2018. Microbiota-derived lactate accelerates intestinal stem cell-mediated epithelial development through the Gpr81. Cell Host and Microbe. 24(6):833-846. https://doi.org/10.1016/j.chom.2018.11.002. - Discovery of an acidic, thermostable and highly NADP+ dependent formate dehydrogenase from Lactobacillus buchneri NRRL B-30929 -(Peer Reviewed Journal)
Alpdagtas, S., Yücel, S., Kapkaç, H.A., Liu, S., Binay, B. 2018. Discovery of an acidic, thermostable and highly NADP+ dependent formate dehydrogenase from Lactobacillus buchneri NRRL B-30929. Biotechnology Letters. 40(7): 1135-1147. doi: 10.1007/s10529-018-2568-6. - Butanol production from sweet sorghum bagasse with high solids content: Part I – comparison of liquid hot water pretreatment with dilute sulfuric acid -(Peer Reviewed Journal)
Qureshi, N., Saha, B.C., Klasson, K.T., Liu, S. 2018. Butanol production from sweet sorghum bagasse with high solids content: Part I – comparison of liquid hot water pretreatment with dilute sulfuric acid. Biotechnology Progress. 34(4):960-966. https://doi.org/10.1002/btpr.2639 - High solid fed-batch butanol fermentation with simultaneous product recovery: Part II - process integration. -(Peer Reviewed Journal)
Qureshi, N., Saha, B.C., Klasson, K.T., Liu, S. 2018. High solid fed-batch butanol fermentation with simultaneous product recovery: Part II - process integration. Biotechnology Progress. 34(4):967-972. https://doi.org/10.1002/btpr.2643 - Fermentative production of butyric acid from paper mill sludge hydrolysates using Clostridium tyrobutyricum NRRL B-67062/RPT 4213 -(Peer Reviewed Journal)
Liu, S., Duncan, S., Qureshi, N., Rich, J.O. 2018. Fermentative production of butyric acid from paper mill sludge hydrolysates using Clostridium tyrobutyricum NRRL B-67062/RPT 4213. Biocatalysis and Agricultural Biotechnology. 14:48-51. https://doi.org/10.1016/j.bcab.2018.02.002. - Production of acetone butanol ethanol (ABE) from Physaria fendleri press cake using Clostridium beijerinckii
-(Abstract Only)
- Integrated production of second generation ethanol and lactic acid from steam-exploded elephant grass -(Peer Reviewed Journal)
Montipó, S., Ballesteros, I., Fontana, R.C., Liu, S., Martins, A.F., Ballesteros, M., Camassola, M. 2017. Integrated production of second generation ethanol and lactic acid from steam-exploded elephant grass. Bioresource Technology. 249:1017-1024. doi: 10.1016/j.biortech.2017.11.001. - Resolving bacterial contamination of fuel ethanol fermentations with beneficial bacteria – an alternative to antibiotic treatment -(Peer Reviewed Journal)
Rich, J.O., Bischoff, K.M., Leathers, T.D., Anderson, A.M., Liu, S., Skory, C.D. 2017. Resolving bacterial contamination of fuel ethanol fermentations with beneficial bacteria – an alternative to antibiotic treatment. Bioresource Technology. 247:357-362. https://doi.org/10.1016/j.biortech.2017.09.067. - Butanol biorefineries: Use of novel technologies to produce biofuel butanol from sweet sorghum bagasse (SSB)-(Abstract Only)
Qureshi, N., Klasson, K.T., Boddu, V.M., Liu, S., Nichols, N.N., Saha, B.C., Hughes, S.R. 2017. Butanol biorefineries: Use of novel technologies to produce biofuel butanol from sweet sorghum bagasse (SSB) [abstract]. Symposium for Biotechnology for Fuels and Chemicals, May 1-4, 2017, San Francisco, CA. - Progress and perspectives on improving butanol tolerance-(Review Article)
Liu, S., Qureshi, N., Hughes, S.R. 2017. Progress and perspectives on improving butanol tolerance. World Journal of Microbiology and Biotechnology 33(3):51. doi: 10.1007/s11274-017-2220-y. - Novel antimicrobial compounds from microbial sources-(Abstract Only)
Bischoff, K.M., Leathers, T.D., Price, N.P., Skory, C.D., Liu, S., Donovan, D.M., Rich, J.O. 2016. Novel antimicrobial compounds from microbial sources [abstract]. Alternative to Antibiotics. - Butanol biorefineries: simultaneous product removal & process integration for conversion of biomass & food waste to biofuel-(Abstract Only)
Qureshi, N., Huang, H., Singh, V., Liu, S., Saha, B.C., Hughes, S.R. 2016. Butanol biorefineries: simultaneous product removal & process integration for conversion of biomass & food waste to biofuel [abstract]. 14th International Conference of Genetics, Physiology, and Synthetic Biology of Solvent-and-Acid Forming Clostridia. August 28-31, 2016, Hanover, New Hampshire. Page 22. No. 017 - Cellulosic butanol biofuel production from sweet sorghum bagasse (SSB): Impact of hot water pretreatment and solid loadings on fermentation employing Clostridium beijerinckii P260 -(Peer Reviewed Journal)
Qureshi, N., Liu, S., Hughes, S., Palmquist, D., Dien, B., Saha, B. 2016. Cellulosic butanol (ABE) biofuel production from sweet sorghum bagasse (SSB): Impact of hot water pretreatment and solid loadings on fermentation employing Clostridium beijerinckii P260. BioEnergy Research. 9(4):1167-1179. doi: 10.1007/s12155-016-9761-z. - Growth, ethanol production, and inulinase activity on various inulin substrates by mutant kluyveromyces marxianus strains NRRL Y-50798 and NRRL Y-50799-(Peer Reviewed Journal)
Galinda-Leva, L.A., Hughes, S.R., Lopez-Nunez, J.C., Jarodsky, J.M., Erickson, A., Lindquist, M.R., Cox, E.J., Bischoff, K.M., Hoecker, E.C., Liu, S., Qureshi, N., Jones, M.A. 2016. Growth, ethanol production, and inulinase activity on various inulin substrates by mutant Kluyveromyces marxianus strains NRRL Y-50798 and NRRL Y-50799. Journal of Industrial Microbiology and Biotechnology. 43(7):927-939. doi: 10.1007/s10295-016-1771-5. - Phylogenetic classification of Aureobasidium pullulans strains for production of feruloyl esterase-(Peer Reviewed Journal)
Rich, J.O., Manitchotpisit, P., Peterson, S.W., Liu, S., Leathers, T.D., Anderson, A.M. 2016. Phylogenetic classification of Aureobasidium pullulans strains for production of feruloyl esterase. Biotechnology Letters. 38(5):863-870. - Identification of butanol tolerant genes in Lactobacillus mucosae-(Abstract Only)
Liu, S., Qureshi, N. 2016. Identification of butanol tolerant genes in Lactobacillus mucosae [abstract]. American Society for Microbiology. - The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance-(Peer Reviewed Journal)
Liu, S., Skory, C., Qureshi, N., Hughes, S. 2016. The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance. Journal of Industrial Microbiology and Biotechnology. 43(4):441-450. doi: 10.1007/s10295-015-1730-6. - Novel feruloyl esterase from Lactobacillus fermentum NRRL B-1932 and analysis of the recombinant enzyme produced in Escherichia coli.-(Peer Reviewed Journal)
Liu, S., Bischoff, K.M., Anderson, A.M., Rich, J.O. 2016. Novel feruloyl esterase from Lactobacillus fermentum NRRL B-1932 and analysis of the recombinant enzyme produced in Escherichia coli. Applied and Environmental Microbiology. 82(17):5068-5076. doi: 10.1128/AEM.01029-16. - Irradiation of Yarrowia lipolytica NRRL YB-567 creating novel strains with enhanced ammonia and oil production on protein and carbohydrate substrates-(Peer Reviewed Journal)
Lindquist, M.R., Lopez-Nunez, J.C., Jones, M.A., Cox, E.J., Pinkleman, R.J., Bang, S.S., Moser, B.R., Jackson, M.A., Iten, L.B., Kurtzman, C.P., Bischoff, K.M., Liu, S., Qureshi, N., Tasaki, K., Rich, J.O., Cotta, M.A., Saha, B.C., Hughes, S.R. 2015. Irradiation of Yarrowia lipolytica NRRL YB-567 creating novel strains with enhanced ammonia and oil production on protein and carbohydrate substrates. Applied Microbiology and Biotechnology. 99(22):9723–9743. - Novel ferulate esterase from Gram-positive lactic acid bacteria and analyses of the recombinant enzyme produced in E. coli-(Abstract Only)
Liu, S., Bischoff, K.M. 2015. Novel ferulate esterase from Gram-positive lactic acid bacteria and analyses of the recombinant enzyme produced in E. coli. [abstract]. American Society for Microbiology. Poster #305, p. 115. - Genetically engineered Escherichia coli FBR5 to use cellulosic sugars: Production of ethanol from corn fiber hydrolyzate employing commercial nutrient medium -(Peer Reviewed Journal)
Qureshi, N., Dien, B.S., Saha, B.C., Iten, L., Liu, S., Hughes, S.R. 2015. Genetically engineered Escherichia coli FBR5 to use cellulosic sugars: Production of ethanol from corn fiber hydrolyzate employing commercial nutrient medium. European Chemical Bulletin. 4(3):130-134. https://doi.org/10.17628/ecb.2015.4.130-134 - Development of synthetic chromosomes and improved microbial strains to utilize cellulosic feedstocks and express valuable coproducts for sustainable production of biofuels from corn-(Abstract Only)
Hughes, S.R., Bischoff, K.M., Liu, S., Rich, J.O. 2015. Development of synthetic chromosomes and improved microbial strains to utilize cellulosic feedstocks and express valuable coproducts for sustainable production of biofuels from corn [abstract]. Corn Processing Workshop and International Starch Technology Conference. - Process for assembly and transformation into Saccharomyces cerevisiae of a synthetic yeast artificial chromosome containing a multigene cassette to express enzymes that enhance xylose utilization designed for an automated pla -(Peer Reviewed Journal)
Hughes, S.R., Cox, E.J., Bang, S.S., Pinkelman, R.J., Lopez-Nunez, J.C., Saha, B.C., Qureshi, N., Gibbons, W.R., Fry, M.R., Moser, B.R., Bischoff, K.M., Liu, S., Sterner, D.E., Butt, T.R., Reidmuller, S.B., Jones, M.A., Riano-Herrera, N.M. 2015. Process for assembly and transformation into Saccharomyces cerevisiae of a synthetic yeast artificial chromosome containing a multigene cassette to express enzymes that enhance xylose utilization designed for an automated platform. Journal of Laboratory Automation. 20(6):621-635. doi: 10.1177/2211068215573188. - Functional analyses of the cell wall hydrolase from Lactobacillus paracasei NRRL B-50314 expressed in Bacillus megaterium-(Peer Reviewed Journal)
Liu, S., Rich, J.O., Anderson, A.M. 2014. Antibacterial activity of a cell wall hydrolase from Lactobacillus paracasei NRRL B-50314 produced by recombinant Bacillus megaterium. Journal of Industrial Microbiology and Biotechnology. 42:229-235. - Proteomic analyses of ethanol tolerance in Lactobacillus buchneri NRRL B-30929 -(Peer Reviewed Journal)
Liu, S. 2014. Proteomic analyses of ethanol tolerance in Lactobacillus buchneri NRRL B-30929. Proteomics. 14(21-22):2540-2544. - Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium sp. strain RPT-4213-(Abstract Only)
Liu, S., Bischoff, K.M., Leathers, T.D., Qureshi, N., Rich, J.O., Hughes, S.R. 2014. Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium sp. strain RPT-4213 [abstract]. Society for Industrial Microbiology and Biotechnology. p. 24. - Proteomic Analyses of Ethanol Tolerance in Lactobacillus buchneri NRRL B-30929-(Abstract Only)
Liu, S. 2014. Proteomic Analyses of Ethanol Tolerance in Lactobacillus buchneri NRRL B-30929 [abstract]. American Society for Microbiology. - Mixed sugar fermentation by Clostridia and metabolic engineering for butanol production-(Book / Chapter)
Ezeji, T.C., Liu, S., Qureshi, N. 2014. Mixed sugar fermentation by Clostridia and metabolic engineering for butanol production. In: Qureshi, N., Hodge, D., Vertes, A., editors. Biorefineries: Integrated Biochemical Processes for Liquid Biofuels. Amsterdam, The Netherlands: Elsevier. p. 191-204. - Process integration for simultaneous saccharification, fermentation, and recovery (SSFR): Production of butanol from corn stover using Clostridium beijerinckii P260-(Peer Reviewed Journal)
Qureshi, N., Singh, V., Liu, S., Ezeji, T.C., Saha, B.C., Cotta, M.A. 2014. Process integration for simultaneous saccharification, fermentation, and recovery (SSFR): Production of butanol from corn stover using Clostridium beijerinckii P260. Bioresource Technology. 154:222-228. - Production of a yeast artificial chromosome for stable expression of a synthetic xylose isomerase-xylulokinase polyprotein in a fuel ethanol yeast strain-(Abstract Only)
Hughes, S.R., Cox, E.J., Bang, S.S., Pinkelman, R., Qureshi, N., Johnson, T.J., Bischoff, K.M., Liu, S., Rich, J.O., Cotta, M.A. 2013. Production of a yeast artificial chromosome for stable expression of a synthetic xylose isomerase-xylulokinase polyprotein in a fuel ethanol yeast strain [abstract]. The Center for Bioprocessing Research and Development. - Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium tyrobutyricum strain RPT-4213 -(Peer Reviewed Journal)
Liu, S., Bischoff, K.M., Leathers, T.D., Qureshi, N., Rich, J.O., Hughes, S.R. 2013. Butyric acid from anaerobic fermentation of lignocellulosic biomass hydrolysates by Clostridium tyrobutyricum strain RPT-4213. Bioresource Technology. 143:322-329. - Development of mutated Kluyveromyces marxianus strains for ethanol production at elevated temperature from biomass hydrolysate-(Abstract Only)
Hughes, S.R., Cox, E.J., Kurtzman, C.P., Bischoff, K.M., Liu, S., Cote, G.L., Rich, J.O., Bang, S.S., Pinkleman, R., Riano-Herrera, N.M., Rodriguez-Valencia, N., Lopez-Nunez, J.C. 2013. Development of mutated Kluyveromyces marxianus strains for ethanol production at elevated temperature from biomass hydrolysate [abstract]. Center for Process Analysis and Control. - Automated UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 and selection for microaerophilic growth and ethanol production at elevated temperature on biomass sugars-(Peer Reviewed Journal)
Hughes, S.R., Bang, S.S., Cox, E.J., Schoepke, A., Ochwat, K., Pinkelman, R., Nelson, D., Qureshi, N., Gibbons, W.R., Kurtzman, C.P., Bischoff, K.M., Liu, S., Cote, G.L., Rich, J.O., Jones, M.A., Cedeno, D., Doran-Peterson, J., Riano, N.M. 2013. Automated UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 and selection for microaerophilic growth and ethanol production at elevated temperature on biomass sugars. Journal of Laboratory Automation. 18(4):276-290. - Renewable and sustainable transportation fuels-(Other)
Liu, S. 2012. Renewable and sustainable transportation fuels. Journal of Petroleum & Environmental Biotechnology. 3(6). DOI: org/10.4172/2157-7463.1000e115. - Novel antibacterial polypeptide produced by Lactobacillus paracasei strain NRRL B-50314-(Abstract Only)
Liu, S., Wilkinson, B.J., Bischoff, K.M., Hughes, S.R., Rich, J.O., Cotta, M.A. 2012. Novel antibacterial polypeptide produced by Lactobacillus paracasei strain NRRL B-50314 [abstract]. American Society for Microbiology. Paper #2512. p. 202. - Genetically engineered Escherichia coli FBR5: Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose-(Peer Reviewed Journal)
Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Hector, R.E., Cotta, M.A., Hughes, S.R. 2012. Genetically engineered Escherichia coli FBR5: Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose. Biotechnology Progress. 28(5):1167-1178. - Genetically engineered Escherichia coli FBR5: Part II. Ethanol production from xylose and simultaneous product recovery-(Peer Reviewed Journal)
Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Cotta, M.A., Hughes, S.R., Hector, R.E. 2012. Genetically engineered Escherichia coli FBR5: Part II. Ethanol production from xylose and simultaneous product recovery. Biotechnology Progress. 28(5):1179-1185. - Novel antibacterial polypeptide laparaxin produced by Lactobacillus paracasei strain NRRL B-50314 via fermentation-(Peer Reviewed Journal)
Liu, S., Wilkinson, B.J., Bischoff, K.M., Hughes, S.R., Rich, J.O., Cotta, M.A. 2012. Novel antibacterial polypeptide laparaxin produced by Lactobacillus paracasei strain NRRL B-50314 via fermentation. J Pet Environ Biotechnol. 3:121. DOI: 10.4172/2157-7463. - Design and construction of a first-generation high-throughput integrated molecular biology platform for production of optimized synthetic genes and improved industrial strains-(Abstract Only)
Hughes, S.R., Butt, T.R., Bartolett, S., Riedmuller, S., Sterner, D., Farrelly, P., Bischoff, K.M., Liu, S., Rich, J.O. 2012. Design and construction of a first-generation high-throughput integrated molecular biology platform for production of optimized synthetic genes and improved industrial strains [abstract]. CBRD Advisory Board. - Production of biodiesel by transesterification of corn and soybean oils with ethanol or butanol using resin-bound truncated Candida antarctica lipase B-(Peer Reviewed Journal)
Hughes, S.R., Moser, B.R., Robinson, S., Cox, E.J., Harmsen, A.J., Friesen, J.A., Bischoff, K.M., Jones, M.A., Pinkleman, R., Bang, S.S., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson, Jr., J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2012. Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol. Journal of Biotechnology. 159:69-77. DOI: 10.1016/j.jbiotec.2012.01.025. - Cellulosic butanol production from agricultural biomass and residues: Recent advances in technology-(Book / Chapter)
Qureshi, N., Liu, S., Ezeji, T.C. 2012. Cellulosic butanol production from agricultural biomass and residues: Recent advances in technology. In: Lee, J.W., editor. Advanced Biofuels and Bioproducts. New York, NY: Springer Science and Business Media. p. 247-265. - Why engineering lactic acid bacteria for biobutanol-(Abstract Only)
Liu, S. 2011. Why engineering lactic acid bacteria for biobutanol [abstract]? Society for Industrial Microbiology. p. 75. - Adaptation of lactic acid bacteria to butanol-(Peer Reviewed Journal)
Liu, S., Bischoff, K.M., Leathers, T.D., Qureshi, N., Rich, J.O., Hughes, S.R. 2012. Adaptation of lactic acid bacteria to butanol. Biocatalysis and Agricultural Biotechnology. 1(1):57-61. DOI: http://dx.doi.org/10.1016/j.bcab.2011.08.008. - Random UV-C mutagenesis of Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars-(Peer Reviewed Journal)
Hughes, S.R., Bischoff, K.M., Gibbons, W.R., Bang, S.S., Pinkelman, R., Slininger, P.J., Qureshi, N., Liu, S., Saha, B.C., Jackson, J.S., Cotta, M.A., Rich, J.O., Javers, J. 2012. Random UV-C mutagenesis of Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars. Journal of Industrial Microbiology and Biotechnology. 39(1):163–173. - High-throughput automated molecular biology platform for production of fuel ethanol yeast capable of expressing high-value heterologous proteins-(Proceedings)
Hughes, S.R., Moser, B.R., Harmsen, A.J., Bischoff, K.M., Jones, M.A., Pinkleman, R., Bang, S.S., Doll, K.M., Tasaki, K., Qureshi, N., Liu, S., Saha, B.C., Jackson, Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2011. High-throughput automated molecular biology platform for production of fuel ethanol yeast capable of expressing high-value heterologous proteins. Proceedings of the Seventh International Starch Technology Conference. p. 21-29. - Purification and characterization of arabinofuranosidase from the corn endophyte Acremonium zeae-(Peer Reviewed Journal)
Bischoff, K.M., De Rezende, S.T., Larson, T.M., Liu, S., Hughes, S.R., Rich, J.O. 2011. Purification and characterization of arabinofuranosidase from the corn endophyte Acremonium zeae. Biotechnology Letters. 33(10):2013-2018. DOI: 10.1007/s10529-011-0658-9. - Complete genome sequence of Lactobacillus buchneri NRRL B-30929, a novel strain from a commercial ethanol plant-(Peer Reviewed Journal)
Liu, S., Leathers, T.D., Copeland, A., Chertkov, O., Goodwin, L., Mills, D.A. 2011. Complete genome sequence of Lactobacillus buchneri NRRL B-30929, a novel strain from a commercial ethanol plant. Journal of Bacteriology. 193(15):4019-4020. DOI: 10.1128/JB.05180-11. - Exploring Lactobacillus buchneri for Biomass to Biofuels-(Abstract Only)
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2010. Exploring Lactobacillus buchneri for biomass to biofuels [abstract]. Symposium on Biocatalysis and Biotechnology. p. 59. - Novel developments in butanol fermentation: Microbial genetics to agricultural substrates, process technology, and downstream processing-(Abstract Only)
Qureshi, N., Liu, S., Saha, B.C., Cotta, M.A. 2010. Novel developments in butanol fermentation: Microbial genetics to agricultural substrates, process technology, and downstream processing [abstract]. In: Proceedings of the 2010 American Institute of Chemical Engineers Midwest Regional Conference, September 30-October 1, 2010, Chicago, Illinois. p. 40. - Purification and Characterization of Two Arabinofuranosidases from the Maize Endophyte Acremonium zeae-(Abstract Only)
Bischoff, K.M., De Rezenda, S.T., Larson, T.M., Liu, S., Hughes, S.R., Rich, J.O. 2010. Purification and characterization of two Arabinofuranosidases from the maize endophyte Acremonium zeae [abstract]. Society for Industrial Microbiology. Poster No. P-12. p. 108. - Functional Expression of the Thiolase Gene thl from Clostridium beijerinckii P260 in Lactococcus lactis and Lactobacillus buchneri-(Abstract Only)
Liu, S., Bischoff, K.M., Hughes, S.R., Qureshi, N., Rich, J.O. 2010. Functional expression of the Thiolase gene THL from Clostridium beijerinckii P260 in Lactococcus lactis and Lactobacillus buchneri [abstract]. Genetics of Industrial Microorganisms. p. 36. - Production of Truncated Candida antarctica Lipase B Gene Using Automated PCR Gene Assembly Protocol and Expression in Yeast for use in Ethanol and Biodiesel Production.-(Abstract Only)
Hughes, S.R., Moser, B.R., Harmsen, A.J., Robinson, S., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.B., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2010. Production of truncated Candida antarctica Lipase B gene using automated PCR gene assembly protocol and expression in yeast for use in ethanol and biodiesel production [abstract]. Advanced Biofuels Leadership Conference. Talk 3. p. 5. - Production of Candida antaractica Lipase B Gene Open Reading Frame using Automated PCR Gene Assembly Protocol on Robotic Workcell & Expression in Ethanologenic Yeast for use as Resin-Bound Biocatalyst in Biodiesel Production-(Peer Reviewed Journal)
Hughes, S.R., Moser, B.R., Harmsen, A.J., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.S., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2010. Production of Candida antaractica Lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production. Journal of the Association for Laboratory Automation. 16(1):17-37. DOI: 10.1016/j.jala.2010.04.002. - Functional Expression of the Thiolase Gene thl from Clostridium beijerinckii p260 in Lactococcus lactis and Lactobacillus buchneri-(Peer Reviewed Journal)
Liu, S., Bischoff, K.M., Qureshi, N., Hughes, S.R., Rich, J.O. 2010. Functional expression of the thiolase gene THl from Clostridium beijerinckii P260 in Lactococcus lactis and Lactobacillus buchneri. New Biotechnology. 27(4):283-288. - Fermentation of Corn Fiber Hydrolysate to Lactic Acid by the Moderate Thermophile Bacillus coagulans-(Peer Reviewed Journal)
Bischoff, K.M., Liu, S., Hughes, S.R., Rich, J.O. 2010. Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile Bacillus coagulans. Biotechnology Letters. 32:823-828. - Production of Value-added Products by Lactic Acid Bacteria-(Book / Chapter)
Liu, S., Li, Y., Azaizeh, H., Cui, F., Tafesh, A., Bischoff, K.M. 2010. Production of value-added products by lactic acid bacteria. In: Hou, C.T., Shaw, J.-F., editors. Biocatalysis and Molecular Engineering. New York: John Wiley and Sons. p. 421-435. - Production of Butanol (a Biofuel) from Agricultural Residues: Part II - Use of Corn Stover and Switchgrass Hydrolysates-(Peer Reviewed Journal)
Qureshi, N., Saha, B.C., Hector, R.E., Dien, B., Hughes, S., Liu, S., Iten, L., Bowman, M.J., Sarath, G., Cotta, M.A. 2010. Production of butanol (a Biofuel) from agricultural residues: Part II - Use of corn stover and switchgrass hydrolysates. Biomass and Bioenergy. 34(4):566-571. - Lactic Acid Bateria - Friend or Foe? Lactic Acid Bacteria in the Production of Polysaccharides and Fuel Ethanol-(Abstract Only)
Rich, J.O., Liu, S., Leathers, T.D., Bischoff, K.M. 2009. Lactic acid bacteria - Friend or Foe? Lactic acid bacteria in the production of polysaccharides and fuel ethanol [abstract]. Conference on Lactic Acid Bacteria in Thai Food and Feed Industries. Paper L01. p. 69. - Lactic Acid Bacteria – Friend or Foe? Lactic Acid Bacteria in the Production of Polysaccharides and Fuel Ethanol-(Peer Reviewed Journal)
Rich, J.O., Bischoff, K.M., Leathers, T.D., Cote, G.L., Liu, S. 2010. Lactic acid bacteria – Friend or Foe? Lactic acid bacteria in the production of Polysaccharides and fuel ethanol. KKU Research Journal. 15(5):424-435. - How Microbes Tolerate Ethanol and Butanol-(Review Article)
Liu, S., Qureshi, N. 2009. How microbes tolerate ethanol and butanol. New Biotechnology. 26(3/4):117-121. - Industrial Saccharomyces cerevisiae Yeast Strain Engineered to Convert Glucose, Mannose, Arabinose, and Xylose (GMAX) to Ethanol Anaerobically-(Abstract Only)
Hughes, S.R., Qureshi, N., Butt, T., Bang, S., Bischoff, K.M., Saha, B.C., Liu, S., Rich, J.O. 2009. Industrial Saccharomyces cerevisiae yeast strain engineered to convert glucose, mannose, arabinose, and xylose (GMAX) to ethanol anaerobically [abstract]. American Institute of Chemical Engineers. Talk #2. p. 1. - GMAX-L Saccharomyces Cerevisiae Strains for Profitable Sustainable Cellulosic Ethanol and Biodiesel Production Concurrently using Engineered Workcell-(Abstract Only)
Hughes, S.R., Moser, B.R., Doll, K.M., Tasaki, K., Harmsen, A., Jones, M., Bang, S., Gibbons, W., Butt, T. 2009. GMAX-L Saccharomyces cerevisiae strains for profitable sustainable cellulosic ethanol and biodiesel production concurrently using engineered workcell [abstract]. Center for Process Analytical Chemisty. Abstract No. 2. p. 1. - Automated High Throughput GMAX-L Strains of Saccharomyces Cereviciae for Profitable Cellulosic Ethanol Production from Industrial Hydrosylates-(Abstract Only)
Hughes, S.R., Tasaki, K., Doll, K.M., Moser, B.R., Rich, J.O., Qureshi, N., Hector, R.E., Dien, B.S., Cotta, M.A., Bischoff, K.M., Liu, S., Bang, S., Jones, M. 2009. Automated high throughput GMAX-L strains of Saccharomyces cereviciae for profitable cellulosic ethanol production from industrial hydrosylates [abstract]. Society for In-Vitro Biology. p. 122. - Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen-(Peer Reviewed Journal)
Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen. Journal of the Association for Laboratory Automation. 8:200-212. - Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings-(Abstract Only)
Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42. - Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production-(Peer Reviewed Journal)
Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. Journal of the Association for Laboratory Automation. 8:190-199. - Extracellular Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae-(Peer Reviewed Journal)
Bischoff, K.M., Wicklow, D.T., Jordan, D.B., De Rezende, S.T., Liu, S., Hughes, S.R., Rich, J.O. 2009. Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae. Current Microbiology. 58:499-503. - Modeling Bacterial Contamination of Fuel Ethanol Fermentation-(Peer Reviewed Journal)
Bischoff, K.M., Liu, S., Leathers, T.D., Worthington, R.E., Rich, J.O. 2009. Modeling bacterial contamination of fuel ethanol fermentation. Biotechnology and Bioengineering. 103(1):117-122. - Make use of lactic acid bacteria in biomass to biofuel-(Abstract Only)
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Make use of lactic acid bacteria in biomass to biofuel [abstract]. International Symposium on Biocatalysis and Biotechnology. Post No. M-2. p. 60. - Conversion of Biomass Hydrolysates and Other Substrates to Ethanol and Other Chemicals by Lactobacillus buchneri-(Peer Reviewed Journal)
Liu, S., Bischoff, K.M., Hughes, S.R., Leathers, T.D., Price, N.P., Qureshi, N., Rich, J.O. 2009. Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri. Letters of Applied Microbiology. 48(3):337-342. - Conversion of Biomass to Ethanol by Other Organisms-(Book / Chapter)
Liu, S. 2009. Conversion of biomass to ethanol by other organisms. In: Vertes, A.A., Blascheck, H.P., Yukawa, H., Qureshi, N., editors. Biomass to Biofuels. Chapter 14. West Sussex, UK:John Wiley & Sons, Ltd. p. 293-310. - Three-vector System for use on Automated Systems for High-level Functional Expression of Value-added Co-products with Xylose Isomerase and Xylulokinase in an Industrial Saccharomyces cerevisiae Strain-(Abstract Only)
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D. 2008. Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Indiana Council of Engineering Societies. Abstract 13. p. 4. - Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system-(Peer Reviewed Journal)
Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38. - Improving Biocatalysts for the Production of Biofuels from Lignocellulosic Feedstocks-(Abstract Only)
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving biocatalysts for the production of biofuels from Lignocellulosic feedstocks [abstract]. U.S. Japan Natural Resources Meeting. p. 54-55. - Lactobacillus buchneri: A novel biocatalyst for ethanol production-(Abstract Only)
Liu, S. 2008. Lactobacillus buchneri: A Novel Biocatalyst for Ethanol Production [abstract]. MWA Bioenergy Research Forum. Poster No. 14. - Proteomic Approach for Molecular Mechanisms under Ethanol Stress in Lactobacillus buchneri-(Abstract Only)
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri [abstract]. American Society for Microbiology. 0-030. p. 155. - Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain-(Abstract Only)
Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4. - Controlled Antibiotic use during Fuel Ethanol Production-(Abstract Only)
Bischoff, K.M., Scott, J., Leathers, T.D., Liu, S., Worthington, R. 2008. Controlled antibiotic use during fuel ethanol production [abstract]. National Science Foundation, Partnerships for Innovation Workshop. Poster No. 13. - Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain-(Peer Reviewed Journal)
Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART. - Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri-(Abstract Only)
Liu, S., Bischoff, K.M., Price, N.P., Hughes, S.R. 2007. Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri [abstract]. Symposium on Biocatalysis and Biotechnology. p. 49. - Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products-(Peer Reviewed Journal)
Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2008. Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products. Journal of Industrial Microbiology and Biotechnology. 35:75-81. - Gram-positive bacteria as biocatalysts to convert biomass derived sugars into biofuel and chemicals-(Book / Chapter)
Liu, S., Cotta, M.A. 2007. Gram-positive bacteria as biocatalysts to convert biomass derived sugars into biofuel and chemicals. In: Hou, C.T., Shaw, J.-F., editors. Biocatalysis and Bioenergy. Chapter 14. New Jersey:John Wiley & Sons, Ltd. p. 294-264. - Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis-(Peer Reviewed Journal)
Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., Cotta, M.A. 2007. Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis. FEMS Microbiolology Letters. 274(2):291-297. - Monensin inhibits growth of bacterial contaminants from fuel ethanol plants-(Abstract Only)
Bischoff, K.M., Liu, S., Dien, B.S. 2007. Monensin inhibits growth of bacterial contaminants from fuel ethanol plants [abstract]. American Society for Microbiology. Poster #0-010. p. 60. - Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361-(Peer Reviewed Journal)
Bischoff, K.M., Liu, S., Hughes, S.R. 2007. Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361. Process Biochemistry. 42:1150-1154. - A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products-(Abstract Only)
Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2007. A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products [abstract]. Society of Industrial Microbiology Annual Meeting. p. 158. - High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System-(Peer Reviewed Journal)
Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212. - Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces-(Abstract Only)
Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with improved growth on xylose expressing Xylanase A(Xyn A) and Celulase F(Cel F) from anaerobic fungi Orpinomyces [abstract]. World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19. - Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts.-(Abstract Only)
Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with optimized "Trojan Horse" amino acid scanning mutational inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4. - Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide-(Abstract Only)
Hughes, S.R., Li, X., Liu, S., Bischoff, K.M., Mertens, J.A., Qureshi, N., Riedmuller, S., Bartolett, S., Farrelly, P., Panavas, T., Butt, T., Cotta, M.A. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose and engineered to express an insecticidal peptide [abstract]. PEPTALK 2007, Cambridge Healthtech Institute. Talk 83, p. 7. - DEVELOPING GRAM-POSITIVE ETHANOLOGENS-(Abstract Only)
Liu, S. 2006. Developing gram-positive ethanologens [abstract]. International Symposium of Biocatalysis and Bioenergy. p. 33. - CHROMOSOMAL INACTIVATION OF THE ALS GENE IN LACTOBACILLUS PLANTARUM STRAIN TF103 TO ELIMINATE UNDESIRED FERMENTATION PRODUCTS-(Abstract Only)
Liu, S. 2006. Chromosomal inactivation of the als gene in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products [abstract]. Society of Industrial Microbiology. p. 166. - PURIFICATION AND CHARACTERIZATION OF A FAMILY 5 ENDOGLUCANASE FROM A MODERATELY THERMOPHILIC STRAIN OF BACILLUS LICHENIFORMIS-(Peer Reviewed Journal)
Bischoff, K.M., Rooney, A.P., Li, X., Liu, S., Hughes, S.R. 2006. Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters. 28:1761-1765. - GENETICALLY ENGINEERED LACTIC ACID BACTERIA FOR THE PRODUCTION OF FUELS AND CHEMICALS-(Abstract Only)
Liu, S. 2006. Genetically engineered lactic acid bacteria for the production of fuels and chemicals [abstract]. 10th International Symposium on the Genetics of Industrial Microorganisms. Paper #015. - ISOLATION OF XYLOSE-UTILIZING THERMOPHILIC BACTERIA FROM DAIRY MANURE COMPOST-(Abstract Only)
Bischoff, K.M., Liu, S., Edrington, T.S., Hughes, S.R. 2006. Isolation of xylose-utilizing thermophilic bacteria from dairy manure compost [abstract]. Extremophiles 2006. p. 19. Poster P045. - FUNCTIONAL PROTEOMIC PLASMID-BASED INTEGRATED WORKCELL FOR HIGH-THROUGHPUT TRANSFORMATION OF BL21 DE3 E. COLI FOR EXPRESSION IN VIVO WITH PIROMYCES STRAIN XYLOSE ISOMERASE-(Abstract Only)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2. - A COLD-SENSITIVE LISTERIA MONOCYTOGENES MUTANT HAS A TRANSPOSON INSERTION IN A GENE ENCODING A PUTATIVE MEMBRANE PROTEIN AND SHOWS ALTERED (P)PPGPP LEVELS-(Peer Reviewed Journal)
Liu, S., Bayles, D.O., Mason, T.M., Wilkinson, B.J. 2006. A cold-sensitive Listeria monocytogenes mutant has a transposon insertion in a gene encoding a putative membrane protein and shows altered (p)ppGpp levels. Applied and Environmental Microbiology. 72(6):3955-3959. - MASS TRANSFORMATION OF PLASMID LIBRARIES OF CDNA OR MUTAGENIZED CLONE SETS INTO YEAST OR BACTERIA USING A FUNCTIONAL PROTEOMIC ROBOTIC WORKCELL-(Abstract Only)
Hughes, S.R., Riedmuller, S., Li, X., Qureshi, N., Liu, S., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2006. Mass transformation of plasmid libraries of cDNA or mutagenized clone sets into yeast or bacteria using a functional proteomic robotic workcell [abstract]. PepTalk 2006. p. 10. - CONTINUOUS PRODUCTION OF ETHANOL IN HIGH PRODUCTIVITY BIOREACTORS USING ESCHERICHIA COLI FBR5: MEMBRANE AND FIXED CELL REACTORS-(Abstract Only)
Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using Escherichia coli FBR5: membrane and fixed cell reactors [abstract]. American Institute of Chemical Engineers. Paper No. 589g. - CONTINUOUS PRODUCTION OF ETHANOL IN HIGH PRODUCTIVITY BIOREACTORS USING GENETICALLY ENGINEERED ESCHERICHIA COLI FBR5: MEMBRANE AND FIXED CELL REACTORS-(Abstract Only)
Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Hughes, S.R., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using genetically engineered Escherichia coli FBR5: membrane and fixed cell reactors [extended abstract]. American Institute of Chemical Engineers. Paper No. 589g. - A SIMPLE METHOD TO GENERATE CHROMOSOMAL MUTATIONS IN LACTOBACILLUS PLANTARUM STRAIN TF103 TO ELIMINATE UNDESIRED FERMENTATION PRODUCTS-(Peer Reviewed Journal)
Liu, S. 2006. A simple method to generate chromosomal mutations in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products. Applied Biochemistry and Biotechnology. 129-132:854-863. - CHARACTERIZATION OF CARBOXYMETHYLCELLULASE ACTIVITY FROM GEOBACILLUS STEAROTHERMOPHILUS-(Abstract Only)
Bischoff, K.M., Li, X., Rooney, A.P., Liu, S., Hughes, S.R. 2005. Characterization of carboxymethylcellulase activity from Geobacillus stearothermophilus [abstract]. Society for Industrial Microbiology. Paper #P38. - LACTOBACILLUS BREVIS: A POTENTIAL BIOCATALYST FOR LIGNOCELLULOSIC BIOMASS TO ETHANOL-(Abstract Only)
Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05. - METABOLIC ENGINEERING OF A LACTOBACILLUS PLANTARUM DOUBLE LDH KNOCKOUT STRAIN FOR ENHANCED ETHANOL PRODUCTION-(Peer Reviewed Journal)
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2006. Metabolic engineering of a Lactobacillus plantarum double LDH knockout strain for enhanced ethanol production. Journal of Industrial Microbiology and Biotechnology. 33(1):1-7. - ETHANOL FERMENTATION BY OVEREXPRESSING A GRAM-POSITIVE PDC GENE IN LACTOBACILLUS PLANTARUM STRAIN TF103-(Abstract Only)
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation by overexpressing a Gram-positive PDC gene in Lactobacillus plantarum strain TF103 [abstract]. Biotechnology for Fuels and Chemicals Symposium Proceedings, May 1-4, 2005, Denver, Colorado. p. 158. - METABOLIC ENGINEERING OF LACTOBACILLUS BREVIS FOR ETHANOL PRODUCTION-(Abstract Only)
Liu, S., Dien, B.S. 2005. Metabolic engineering of Lactobacillus brevis for ethanol production [abstract]. The World Congress on Industrial Biotechnology and Bioprocessing. p. 23. - FUNCTIONAL EXPRESSION OF BACTERIAL ZYMOBACTER PALMAE PYRUVATE DECARBOXYLASE GENE IN LACTIC ACID BACTERIA-(Peer Reviewed Journal)
Liu, S., Dien, B.S., Cotta, M.A. 2005. Functional expression of bacterial Zymobacter palmae pyruvate decarboxylase gene in lactic acid bacteria. Current Microbiology. 50:1-6. - CLONING, EXPRESSION, PURIFICATION, AND ANALYSIS OF MANNITOL DEHYDROGENASE GENE MTLK FROM LACTOBACILLUS BREVIS-(Peer Reviewed Journal)
Liu, S., Saha, B.C., Cotta, M.A. 2005. Cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlK from Lactobacillus brevis. Applied Biochemistry and Biotechnology. 121-124:391-402. - EXPRESSION ANALYSIS OF PYRUVATE DECARBOXYLASE GENES FROM ZYMOBACTER PALMAE AND SARCINA VENTRICULI IN GRAM-POSITIVE LACTIC ACID BACTERIA-(Abstract Only)
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2004. Expression analysis of pyruvate decarboxylase genes from Zymobacter palmae and Sarcina ventriculi in Gram-positive lactic acid bacteria [abstract]. American Society for Microbiology. Paper No. O-099. - CLONING, EXPRESSION, PURIFICATION, AND ANALYSIS OF MANNITOL DEHYDROGENASE OF MTLD AND MTLK GENES FROM LACTOBACILLUS PLANTARUM AND LACTOBACILLUS BREVIS-(Abstract Only)
Liu, S., Saha, B.C., Cotta, M.A. 2004. Cloning, expression, purification, and analysis of mannitol dehydrogenase of mtlD and mtlK genes from Lactobacillus plantarum and Lactobacillus brevis [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 2-24. - GENETIC ENGINEERING OF LACTIC ACID BACTERIA TOWARD FERMENTATIVE PRODUCTION OF ETHANOL-(Abstract Only)
Liu, S., Cotta, M.A. 2004. Genetic engineering of lactic acid bacteria toward fermentative production of ethanol [abstract]. American Society for Microbiology. p. 29. - INSIGHTS INTO GROWTH OF LISTERIA MONOCYTOGENES AT REFRIGERATION TEMPERATURES THROUGH STUDIES OF TN917-INDUCED COLD-SENSITIVE MUTANTS-(Abstract Only)
Zhu, K., Liu, F., Liu, S., Wilkinson, B.J. 2003. Insights into growth of Listeria monocytogenes at refrigeration temperatures through studies of Tn917-induced cold-sensitive mutants [abstract]. Great Lake Regional Center of Excellence, First Annual Conference, Northwestern University, December 12-14, 2003, Chicago, Illinois. p. 25.
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