Skip to main content
ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Insect Genetics and Biochemistry Research » Research » Publications at this Location » Publication #58519
Title: LOWER INCUBATION TEMPERATURES INCREASE YIELD OF INSECT GENOMIC DNA ISOLATEDBY THE CTAB METHOD

Author
item SHAHJAHAN, REZA - BANGLADESH ATOMIC ENERGY
item Hughes, Keith
item Leopold, Roger
item Devault, James

Submitted to: Biotechniques
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/17/1995
Publication Date: N/A
Citation: N/A

Interpretive Summary: This report deals with a improvement of a technique for extracting DNA from insects for use in molecular biology studies. The improvement involves running the extraction at temperatures lower than the previously accepted procedure. Conducting the extraction at 37 degrees C rather than 65 or 80 degrees, yields nearly three times the amount of high quality DNA recovered. This modification improves the efficiency of the first critical step in experiments using insect DNA.

Technical Abstract: Extraction of insect genomic DNA using the CTAB method is typically conducted at 65 degrees C. In this report, we show that the CTAB method can be rendered more efficient if the temperature is reduced to 37 degrees C during extraction. Extraction at 37 degrees yielded nearly threefold more high quality DNA than conducting the extraction at 19, 65, or 80 degrees. This modification improves the efficiency of the first critical step in experiments using insect DNA.