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ARS Home » Pacific West Area » Wenatchee, Washington » Physiology and Pathology of Tree Fruits Research » Research » Publications at this Location » Publication #413631

Research Project: Uncovering Rootstock Disease Resistance Mechanisms in Deciduous Tree Fruit Crops and Development of Genetics-Informed Breeding Tools for Resistant Germplasm

Location: Physiology and Pathology of Tree Fruits Research

Title: The expression profiles of MATE and MYB gene families in apple roots during defense activation against infection from Pythium ultimum [abstract]

item Zhu, Yanmin
item Rainbow, Jordan
item Ortiz-Uriarte, Bianca

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/16/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The comprehensive dataset from three consecutive transcriptome analyses, including RNA-seq based comparative transcriptomics, microRNA profiling, and degradome sequencing, have uncovered a multi-phase and multi-layer defense strategy employed by apple roots upon infection from a soilborne necrotrophic oomycete pathogen, Pythium ultimum. One of the primary regulation schemes associated with effective defense activation in apple root tissues involves an upregulated phenylpropanoid biosynthesis pathway, robust transportation of secondary metabolites, and enhanced lignin deposition 48 -72 hpi (hours post inoculation). It is proposed that genes encoding MATE (Multidrug and Toxic Compound Extrusion) transporters and MYB (myeloblastosis related) transcription factors are among the essential components contributing to the observed host resistance traits in young apple roots. The current study examined the expression profiles of members in apple roots for both gene families in apple genome, i.e., 66 MATE genes and 219 MYB genes. Sequence variation within coding regions and composition of cis elements in promoter regions were also examined for selected members among several apple rootstock genotypes. The result from current analysis is critical for selecting target genes for subsequent transgenic study, with the aim of defining their functional roles contributing to apple root resistance traits such as elevated cell wall lignification during defense activation.